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Impact of g-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland

Introduction Cellular proliferation and apoptosis are evolutionary conserved mechanisms vital for coordinating growth at both organ and tissue levels. Critically, the coordinated regulation of cell proliferation and apoptosis is pivotal to preserving organismal homeostasis ( Liebermann et al., 1995 ; Streichan et al., 2014 ). Therefore, any dysregulation in cellular turnover kinetics impacts organ differentiation and function and sequentially predisposes these organs/organ systems to pathology or failure ( Davis and Ryan, 1998 ; Edelblum et al., 2006

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Interaction of plant alkaloid, berberine, with zwitterionic and negatively charged phospholipid bilayers

, 201-205. Jain M. K. & Wu N. M. (1977). Effect of small molecules on the dipalmitoyl lecithin liposomal bilayer: III. Phase transition in lipid bilayer, J. Membr. Biol.   34 , 157-201. Jantova S., Cipak L. & Letasiova S. (2007). Berberine induces apoptosis through a mitochondrial/caspase pathway in human promonocytic U937 cells. Toxicol. In Vitro , 21 , 25-31. Krasnowska E. K., Gratton E. & Parasassi T. (1998). Prodan as a Membrane Surface Fluorescence Probe: Partitioning between Water

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Peptides conformational changes of the erythrocyte membrane induced by organometallic tin compounds

., Müller M., Orrenius S. & Gigliotti D. (2001) Organotin-induced caspase activation and apoptosis in human peripheral blood lymphocytes. Chem. Res. Toxicol. , 14 , 791-798. Surewicz W. K. & Mantsch H. H. (1988) New insight into protein secondary structure from resolution-enhanced infrared spectra. Biochim. Biophys. Acta , 952 , 115-130. Watson J. D., Laskowski R. A., Thornton J. M. (2005) Predicting protein function from sequence and structural data. Curr. Opin. Struct. Biol.   15 , 275

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Challenges of ERAU’s First Suborbital Flight Aboard Blue Origin’s New Shepard M7 for the Cell Research Experiment In Microgravity (CRExIM)

launch to December 8, 2017, with a launch window from December 8 to December 11, 2017. On November 26, 2017, the CRExIM team was notified of a change in the launch date to December 11, 2017. This final launch date was given the day of the scrubbed flight, which the team came to know about 3 min before liftoff. Finally, our payload needed to be rearranged to maximize the science collected on December 12, 2017. Had this launch been scrubbed again, it would have left our team with no science data since the in vitro experiment would have reached an apoptosis stage. Not

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Investigation of Murine T-Cells and Cancer Cells under Thermal Stressors and 2D Slow Rotating System Effects as a Testbed for Suborbital Flights

various animal models ( Choi et al., 2018 ), genetically engineered (humanized mice), as a new alternative for testing the effects of cancer immunotherapy and gaining insights about the behaviors of diverse cancers. Besides the simple tests conducted by our team, there are multiple other assays to assess cells viability, such as apoptosis assays, MTT (colorimetric assay for assessing cell metabolic activity), and many others. Failure to do so may result in unfortunate consequences, such as the lost opportunity to gain valuable scientific knowledge. Relevancy to

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Screening of metabolites from endophytic fungi of some Nigerian medicinal plants for antimicrobial activities

of fungal melanins and their importance for human pathogenic fungi. Fungal Genet. Biol. 2003; 38: 143–158. 81. Wardman P. Indole-3-acetic acids and horseradish peroxidase: a new prodrug/enzyme combination for targeted cancer therapy. Curr Pharm Des 2002; 8(15):1363-1374. 82. Jeong YM, Oh MH, Kim SY, Li H, Yun HY, Baek KJ, Kwon NS, Kim WY, Kim DS. Indole-3 acetic acid/horseradish peroxidase induces apoptosis in TCCSUP human urinary bladder carcinoma cells. Pharmazie 2010; 65(2):122-126. 83. Jones LH, Abdalla DSP, Freitas JC. Effects of indole-3

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Culture cells in a model of microgravity

Abstract

The main objective of the work was to clarify the question - how will cell cultures functional state change after microgravity simulation when the shift in full strength direction takes place? Proliferation processes and apoptosis intensity in cell lines of rat glioma and human fibroblasts were compared in changing the position of flasks with cell culture in relation to the horizon. The detection of apoptosis and necrosis processes was carried out using flow cytofluorimetry. It was found that the change in full strength direction provides an inhibitory effect on tumor glial cells and fibroblasts’ proliferative activity enhances along with inhibition of apoptotic processes. Intensification of apoptotic processes in glioma cells and attenuation of cell death processes in normal cells - fibroblasts - are the result of cell cooperation disturbance.

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The rapid interphase chromosome assay (RICA) implementation: comparison with other PCC methods

Abstract

A report is presented on the advantages of the rapid interphase chromosome assay (RICA) and the difficulties that may be met while implementing this method for application in biological dosimetry. The RICA test can be applied on unstimulated human lymphocytes; this is an advantage in comparison with the dicentric chromosomes or micronucleus tests. In the former two tests, stimulated lymphocytes are examined and hence, 48 h more are needed to obtain cells traversing the cell cycle. Due to the use of unstimulated nondividing cells, higher numbers of cells are available for RICA analysis than for dicentric chromosomes or micronuclei tests. Moreover, the method can be applied after exposure to ionizing radiation doses in excess of 5 Gy. Such doses cause a significant cell cycle delay or result in the loss of G2 phase and mitotic cells because of apoptosis. Therefore, the traditional biodosimetry based on the evaluation of the incidence of damage to chromosomes is very difficult to carry out. This is due to the lack of an adequate number of mitotic cells for analysis. RICA is free of this disadvantage. An automatic microscope can be used to retrieve cell images; automatic image analysis can also be used.

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A potential therapeutic role in multiple sclerosis for stigmast-5,22-dien-3β-ol myristate isolated from Capparis ovata

Abstract

Multiple sclerosis (MS) is an autoimmune disease of the human central nervous system. It is one of the most common neurological disorders around the world and there is still no complete cure for MS. Purification of a terpenoid from Capparis ovata was carried out and its structure was elucidated as stigmast-5,22-dien-3β-ol, myristate (3β,22E-stigmasteryl myristate; SDM) by NMR and mass spectral analyses. No information regarding its any health effect is available in the literature. In the present study, we have described its effects on inflammatory factors such as the expression levels of cytokines, chemokines and adhesion molecules as well as apoptosis/infiltration and myelination in SH-SY5Y cells. The expression levels of proinflammatory or inflammatory cytokines and chemokines such as NF-κB1, CCL5, CXCL9, CXCL10 and HIF1A along with T-cell activating cytokines such as IL-6 and TGFB1 were significantly downregulated with SDM treatment. Moreover, the expression levels of the main myelin proteins such as MBP, MAG and PLP that are essential for healthy myelin architecture were significantly up-regulated. The results presented in this study strongly suggest that the SDM offers a unique possibility to be used with autoimmune diseases, including MS due to its activity on the manipulation of cytokines and the promotion of myelin formation.

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Epilobium angustifolium L.: A medicinal plant with therapeutic properties

-360. 8. Vitalone A, Bordi F, Baldazzi C, Mazzanti G, Saso L, Tita B. Antiproliferative effect on a prostatic epithelial cell line (PZ-HPV-7) by Epilobium angustifolium L. Farmaco 2001; 56 (5-7): 483-489. 9. Vitalone A, Guizzetti M, Costa LG, Tita B. Extracts of various species of Epilobium inhibit proliferation of human prostate cells. J Pharm Pharmacol 2003; 55(5): 683-690. 10. Stolarczyk M, Naruszewicz M, Kiss AK. Extracts from Epilobium sp. herbs induce apoptosis in human hormone-dependent prostate cancer cells by activating the

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