Hong Zang, Dong Ji, Qing Shao, Guang-de Zhou, Deng Pan, Shao-jie Xin, Jing-min Zhao and Guo-feng Chen
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transformation process, HBx promoted the expression of AFP in hepatocytes. The AFP then stimulated the expression of some protooncogenes in hepatocytes by activating the phosphatidylinositol-3 kinase (PI3K)/protein kinase -A (AKT) signaling pathway. Subsequently, intracellular apoptosis signals were inhibited and growth signals were activated, resulting in the survival of the HBV-infected hepatocytes in the body [ 9 ]. In addition, the HBx-induced overexpression of AFP in hepatocytes can stimulate the body to produce inflammatory cytokines, especially interleukin-6 (IL-6
Yu Chen, Li-ming Fu, Xiu-ying Zhao, Jun Zhao and Zhong-ping Duan
Objective To investigate the effects of plasma from patients with acute on chronic liver failure on the proliferation and biotransformation function of C3A cells in vitro, and provide experimental data for C3A cells to be efficiently used in the bioartificial liver system.
Methods C3A cells were incubated in 100% normal human plasma (NHP) and 100% abnormal plasma (AP) from patients with acute on chronic liver failure. Growth morphology of the two groups were observed under inverted microscope and scanning electron microscope. The method of methyl thiazolyl tetrazolium (MTT) was conducted for the proliferation activities of C3A cells. The cellular apoptosis rates were assessed by the flow cytometer. The biotransformation function of cells was evaluated through diazepam metabolic amount assay. The concentrations of epithelial growth factor (EGF), transforming growth factor-α (TGF-α) and interleukin-1 (IL-1) were detected in plasma of the two groups.
Results A: The proliferation activities of C3A cells incubated in 100% AP for 24, 48, 72, 96 and 120 hours were significantly higher than that in 100% NHP (P < 0.01). B: Observation under the inverted microscope indicated that the cells in 100% AP were growing faster than those in 100% NHP after cells attached to the plastic at 24 and 48 hours. The same phenomena was observed under the scanning electronic microscope. C: The C3A cells cultured in both groups of plasma showed the same apoptosis rate at 48 hours and there was no statistical difference. D: The diazepam metabolic value of C3A cells incubated in 100% AP for 24, 72 and 120 hours were lower than that in 100% NHP and were statistically different (P < 0.01). E: The concentrations of TGF-α, EGF and IL-1 in AP were significantly higher than that in NHP (P < 0.01).
Conclusions Compared with normal human plasma, the plasma from patients with acute on chronic liver failure has more obvious effect to facilitate the proliferation of C3A cells, but also decreases partial biotransformation function of C3A cells.
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Correlation between HBx and hepatocellular carcinoma
HBx often restructured in the host cell genome 19 . In recent years, more and more significant advancement in the molecular mechanisms about the role of in HCC. Continuous replication in the host is an important process of HCC. In the process of chronic liver disease, the regeneration of infected hepatocytes leads to the integration of HBx into the host gene. It is
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