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The role of neuronal apoptosis inhibitory protein (NAIP) in acute myeloid leukemia patients

Introduction Acute myeloid leukemia (AML) is an infrequent (1.3%), highly malignant neoplasm responsible for a large number of cancer-related deaths [ 1 , 2 ]. In the USA and other highly developed countries, the incidence has been near stable over the last years and is about 4 cases per 100,000 citizens per year [ 1 , 2 , 3 ]. The median age at diagnosis is 67 years [ 1 , 2 , 3 ]. AML is a heterogeneous and complex disease in which genomic and proteomic alterations and the interactions between them result in various apoptosis abnormalities [ 1 , 2 , 3

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miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90+ HepG2 cells

caspase-3-mediated cleavage of JIP1 during apoptosis. Exp Cell Res 2011; 317 : 1028-39. Rudner J, Elsaesser SJ, Jendrossek V, Huber SM. Anti-apoptotic Bcl-2 fails to form efficient complexes with pro-apoptotic Bak to protect from Celecoxibinduced apoptosis. Biochemical Pharmacol 2011; 81 : 32-42. Kim R, Emi M, Matsuura K, Tanabe K. Therapeutic potential of antisense Bcl-2 as a chemosensitizer for patients with gastric carcinoma. Gan To Kagaku Ryoho. 2005; 32 : 1540-5. Barrezueta LF

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Staurosporine induces different cell death forms in cultured rat astrocytes

References 1. Jiang YG, Peng Y, Koussougbo KS. Necroptosis: a novel therapeutic target for glioblastoma. Med Hypotheses 2011; 76: 350-2. 2. Taylor RC, Cullen SP, Martin SJ. Apoptosis: controlled demolition at the cellular level. Nat Rev Cell Mol Biol 2008; 9: 231-41. 3. Kroemer G, Galluzzi L, Vandenabeele P, Abrams J, Alnemri ES, Baehrecke EH, et al. Classification of cell death: recommendations of the Nomenclature Committee on Cell Death. Cell Death Differ 2009; 16: 3-11. 4

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Chemotherapy increases caspase-cleaved cytokeratin 18 in the serum of breast cancer patients

-52. Strojan P. Cysteine cathepsins and stefins in head and neck cancer: an update of clinical studies. Radiol Oncol 2008; 42 : 69-81. Hickman JA, Beere HM, Wood AC, Waters CM, Parmar R. Mechanisms of cytotoxicity caused by antitumour drugs. Toxicol Lett 1992; 64 : 553-61. Ohmori T, Podack ER, Nishio K, Takahashi M, Miyahara Y, Takeda Y, et al. Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by bcl-2. Biochem Biophys Res Commun 1993; 192 : 30

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Knockdown of stat3 expression by RNAi inhibits in vitro growth of human ovarian cancer

cancer cells. Cancer Res 2000; 60: 1225-8. Zhang J, Shen B, Li Y, Sun Y. STAT3 exerts two-way regulation in the biological effects of IL-6 in M1 leukemia cells. Leuk Res 2001; 25: 463-72. Lee SO, Lou W, Qureshi KM, Mehraein-Ghomi F, Trump DL, et al. RNA interference targeting STAT3 inhibits growth and induces apoptosis of human prostate cancer cells. Prostate 2004; 60: 303-9. Konnikova L, Kotecki M, Kruger MM, Cochran BH. Knock-down of STAT3 expression by RNAi induces apoptosis in

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Biological evaluation of transdichloridoplatinum( II) complexes with 3- and 4-acetylpyridine in comparison to cisplatin


Background. In our previous study we reported the synthesis and cytotoxicity of two trans-platinum(II) complexes: trans-[PtCl2(3-acetylpyridine)2] (1) and trans-[PtCl2(4-acetylpyridine)2] (2), revealing significant cytotoxic potential of 2. In order to evaluate the mechanism underlying biological activity of both trans-Pt(II) isomers, comparative studies versus cisplatin were performed in HeLa, MRC-5 and MS1 cells.

Materials and methods. The cytotoxic activity of the investigated complexes was determined using SRB assay. The colagenolytic activity was determined using gelatin zymography, while the effect of platinum complexes on matrix metalloproteinases 2 and 9 mRNA expression was evaluated by quantitative real-time PCR. Apoptotic potential and cell cycle alterations were determined by FACS analyses. Western blot analysis was used to evaluate the effect on expression of DNA-repair enzyme ERCC1, and quantitative real-time PCR was used for the ERCC1 mRNA expression analysis. In vitro antiangiogenic potential was determined by tube formation assay. Platinum content in intracellular DNA and proteins was determined by inductively coupled plasma-optical emission spectrometry.

Results. Compound 2 displayed an apparent cytoselective profile, and flow cytometry analysis in HeLa cells indicated that 2 exerted antiproliferative effect through apoptosis induction, while 1 induced both apoptosis and necrosis. Action of 1 and 2, as analyzed by quantitative real-time PCR and Western blot, was associated with down-regulation of ERCC1. Both trans-complexes inhibited MMP-9 mRNA expression in HeLa, while 2 significantly abrogated in vitro tubulogenesis in MS1 cells. Conclusions. The ability of 2 to induce multiple and selective in vitro cytotoxic effects encourages further investigations of trans-platinum(II) complexes with substituted pyridines.

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LncRNA PVT1 promotes proliferation and invasion through enhancing Smad3 expression by sponging miR-140-5p in cervical cancer

mortality rates remain high as well as the poor prognosis. Therefore, there is an urgent need to clarify the underlying molecular mechanisms of cervical cancer, which could improve the development of therapeutic strategies against cervical cancer. Long noncoding RNAs (lncRNAs) are a group of noncoding RNAs longer than 200 nucleotides that participate in numerous biological and physiological processes including cell development, survival, differentiation and apoptosis. 5 , 6 , 7 Accumulating evidence also proved that lncRNAs have pivotal roles in the progression of

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KRAS, NRAS, BRAF, HER2 and microsatellite instability in metastatic colorectal cancer – practical implications for the clinician

control processes like cell proliferation, cell differentiation, cell adhesion, apoptosis and cell migration. When they are mutated, the cell will have an increased potential of invasion and metastasis. The main members of the RAS family are KRAS and NRAS mutations. 5 , 6 , 7 These are point mutations in which a single nucleotide base is changed, inserted or deleted from a DNA sequence. Moreover, these are frequently somatic mutations (acquired during lifetime). 8 In the metastatic setting, KRAS mutations occur in approximately 40% of the cases, especially in exon 2

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Multigene expression signatures in early hormone receptor positive HER 2 negative breast cancer

contains 70 genes correlated with evading apoptosis, self-sufficiency in growth signals, insensitivity to anti-growth signals, limitless replicative potential, tissue invasion and metastasis and sustained angiogenesis. 21 A mathematical model is used to calculate score that stratifies patients into low- and high risk group. 20 , 22 The first retrospective validation of MammaPrint was performed by van de Vijver and colleagues, on a consecutive series of 295 BC tumors (lymph node positive and negative). MammaPrint accurately distinguished a good-prognosis group which

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Quercetin, Menadione, Doxorubicin combination as a potential alternative to Doxorubicin monotherapy of acute lymphoblastic leukemia

References [1] Baran I, Cell proliferation versus apoptosis. Mechanisms and particularities under genotoxic or oxidative stress conditions. “Carol Davila” University Publishing House, Bucharest, 2014 [2] Murakami A,Ashida H, Terao J. Multitargeted cancer prevention by quercetin. Cancer Lett 2008;269:315-25 [2] Baran I, Ganea C, Privitera S, Scordino A, Barresi V, Musumeci F, et. al. Detailed analysis of apoptosis and delayed luminescence of human leukemia Jurkat T cells after proton-irradiation and treatments

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