Agnieszka Pluta, Tadeusz Robak, Kamil Brzozowski, Barbara Cebula-Obrzut, Agata Majchrzak, Piotr Pluta, Anna Szmigielska-Kapłon, Olga Grzybowska-Izydorczyk, Magdalena Czemerska, Piotr Stelmach, Piotr Smolewski and Agnieszka Wierzbowska
Acute myeloid leukemia (AML) is an infrequent (1.3%), highly malignant neoplasm responsible for a large number of cancer-related deaths [ 1 , 2 ]. In the USA and other highly developed countries, the incidence has been near stable over the last years and is about 4 cases per 100,000 citizens per year [ 1 , 2 , 3 ]. The median age at diagnosis is 67 years [ 1 , 2 , 3 ]. AML is a heterogeneous and complex disease in which genomic and proteomic alterations and the interactions between them result in various apoptosis abnormalities [ 1 , 2 , 3
Lin Fang, Hai-Bing Zhang, Hua Li, Yong Fu and Guang-Shun Yang
caspase-3-mediated cleavage of JIP1 during apoptosis. Exp Cell Res 2011; 317 : 1028-39.
Rudner J, Elsaesser SJ, Jendrossek V, Huber SM. Anti-apoptotic Bcl-2 fails to form efficient complexes with pro-apoptotic Bak to protect from Celecoxibinduced apoptosis. Biochemical Pharmacol 2011; 81 : 32-42.
Kim R, Emi M, Matsuura K, Tanabe K. Therapeutic potential of antisense Bcl-2 as a chemosensitizer for patients with gastric carcinoma. Gan To Kagaku Ryoho. 2005; 32 : 1540-5.
Engin Ulukaya, Esra Karaagac, Ferda Ari, Arzu Oral, Saduman Adim, Asuman Tokullugil and Türkkan Evrensel
Strojan P. Cysteine cathepsins and stefins in head and neck cancer: an update of clinical studies. Radiol Oncol 2008; 42 : 69-81.
Hickman JA, Beere HM, Wood AC, Waters CM, Parmar R. Mechanisms of cytotoxicity caused by antitumour drugs. Toxicol Lett 1992; 64 : 553-61.
Ohmori T, Podack ER, Nishio K, Takahashi M, Miyahara Y, Takeda Y, et al. Apoptosis of lung cancer cells caused by some anti-cancer agents (MMC, CPT-11, ADM) is inhibited by bcl-2. Biochem Biophys Res Commun 1993; 192 : 30
Shu-Hua Zhao, Fan Zhao, Jing-Ying Zheng, Li-Fang Gao, Xue-Jian Zhao and Man-Hua Cui
cancer cells. Cancer Res 2000; 60: 1225-8.
Zhang J, Shen B, Li Y, Sun Y. STAT3 exerts two-way regulation in the biological effects of IL-6 in M1 leukemia cells. Leuk Res 2001; 25: 463-72.
Lee SO, Lou W, Qureshi KM, Mehraein-Ghomi F, Trump DL, et al. RNA interference targeting STAT3 inhibits growth and induces apoptosis of human prostate cancer cells. Prostate 2004; 60: 303-9.
Konnikova L, Kotecki M, Kruger MM, Cochran BH. Knock-down of STAT3 expression by RNAi induces apoptosis in
Lana Filipovic, Sandra Arandelovic, Nevenka Gligorijevic, Ana Krivokuca, Radmila Jankovic, Tatjana Srdic-Rajic, Gordana Rakic, Zivoslav Tesic and Sinisa Radulovic
Background. In our previous study we reported the synthesis and cytotoxicity of two trans-platinum(II) complexes: trans-[PtCl2(3-acetylpyridine)2] (1) and trans-[PtCl2(4-acetylpyridine)2] (2), revealing significant cytotoxic potential of 2. In order to evaluate the mechanism underlying biological activity of both trans-Pt(II) isomers, comparative studies versus cisplatin were performed in HeLa, MRC-5 and MS1 cells.
Materials and methods. The cytotoxic activity of the investigated complexes was determined using SRB assay. The colagenolytic activity was determined using gelatin zymography, while the effect of platinum complexes on matrix metalloproteinases 2 and 9 mRNA expression was evaluated by quantitative real-time PCR. Apoptotic potential and cell cycle alterations were determined by FACS analyses. Western blot analysis was used to evaluate the effect on expression of DNA-repair enzyme ERCC1, and quantitative real-time PCR was used for the ERCC1 mRNA expression analysis. In vitro antiangiogenic potential was determined by tube formation assay. Platinum content in intracellular DNA and proteins was determined by inductively coupled plasma-optical emission spectrometry.
Results. Compound 2 displayed an apparent cytoselective profile, and flow cytometry analysis in HeLa cells indicated that 2 exerted antiproliferative effect through apoptosis induction, while 1 induced both apoptosis and necrosis. Action of 1 and 2, as analyzed by quantitative real-time PCR and Western blot, was associated with down-regulation of ERCC1. Both trans-complexes inhibited MMP-9 mRNA expression in HeLa, while 2 significantly abrogated in vitro tubulogenesis in MS1 cells. Conclusions. The ability of 2 to induce multiple and selective in vitro cytotoxic effects encourages further investigations of trans-platinum(II) complexes with substituted pyridines.
Qing-Qing Chang, Chun-Yan Chen, Zhao Chen and Shuai Chang
mortality rates remain high as well as the poor prognosis. Therefore, there is an urgent need to clarify the underlying molecular mechanisms of cervical cancer, which could improve the development of therapeutic strategies against cervical cancer.
Long noncoding RNAs (lncRNAs) are a group of noncoding RNAs longer than 200 nucleotides that participate in numerous biological and physiological processes including cell development, survival, differentiation and apoptosis. 5 , 6 , 7 Accumulating evidence also proved that lncRNAs have pivotal roles in the progression of
Vlad-Adrian Afrăsânie, Mihai Vasile Marinca, Teodora Alexa-Stratulat, Bogdan Gafton, Marius Păduraru, Anca Maria Adavidoaiei, Lucian Miron and Cristina Rusu
control processes like cell proliferation, cell differentiation, cell adhesion, apoptosis and cell migration. When they are mutated, the cell will have an increased potential of invasion and metastasis. The main members of the RAS family are KRAS and NRAS mutations. 5 , 6 , 7 These are point mutations in which a single nucleotide base is changed, inserted or deleted from a DNA sequence. Moreover, these are frequently somatic mutations (acquired during lifetime). 8 In the metastatic setting, KRAS mutations occur in approximately 40% of the cases, especially in exon 2
contains 70 genes correlated with evading apoptosis, self-sufficiency in growth signals, insensitivity to anti-growth signals, limitless replicative potential, tissue invasion and metastasis and sustained angiogenesis. 21 A mathematical model is used to calculate score that stratifies patients into low- and high risk group. 20 , 22
The first retrospective validation of MammaPrint was performed by van de Vijver and colleagues, on a consecutive series of 295 BC tumors (lymph node positive and negative). MammaPrint accurately distinguished a good-prognosis group which
Ruxandra Irimia, Ioana Teodora Tofolean, Roxana Gabriela Sandu, Oana Elena Băran, Maria Cătălina Ceauşescu, Vlad Coşoreanu, Maria Teodora Ilie, Ramona Babeş, Constanţa Ganea and Irina Băran
 Baran I, Cell proliferation versus apoptosis. Mechanisms and particularities under genotoxic or oxidative stress conditions. “Carol Davila” University Publishing House, Bucharest, 2014
 Murakami A,Ashida H, Terao J. Multitargeted cancer prevention by quercetin. Cancer Lett 2008;269:315-25
 Baran I, Ganea C, Privitera S, Scordino A, Barresi V, Musumeci F, et. al. Detailed analysis of apoptosis and delayed luminescence of human leukemia Jurkat T cells after proton-irradiation and treatments