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A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators

A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators

In an attempt to identify novel and apoptosis/pathogen-regulated microRNAs (miRNAs) and small interfering RNAs, we performed a robust microarray screening of small RNA population from Vitis vinifera L. cv. Limberger cell suspension exposed to apoptosis activators (e.g. methyl jasmonate) or elicitors (botrycin and cinerein) derived from necrotrophic fungus Botrytis cinerea Pers. et Fries. Using a microarray expression profiling approach, we identified 22 miRNAs. We found that a majority of these miRNAs were predicted to target stress/defense-related genes of plants. Of the 22 V. vinifera miRNAs, 11 have sequence conservation in Arabidopsis thaliana but exhibited species-specific developmental and/or stress/defense-related expression patterns. Ten of the miRNAs are highly conserved in other plant species, suggesting that even conserved miRNAs may have different regulatory roles in various species. Our results show that these grapevine miRNAs can be also induced by various apoptosis inducers. Fifty-one potential targets were predicted to the newly identified miRNAs based on sequence complementarity. In addition to miRNAs, we identified 102 other novel endogenous small RNAs in Vitis, indicating that a large number of miRNAs and other small regulatory RNAs are encoded by the Vitis vinifera genome.

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Post-Transcriptional Gene Silencing Conferred by the Ectopic Expression of the Grapevine miRNA-g1 and Inhibition of the Response by Anti-miRNA-g1 Inhibitor

apoptosis is required for N-linked glycosylation. In Genes Cells , vol. 2 , 1997, no. 2, pp. 129-141, DOI: 10.1046/j.1365-2443.1997.1070303.x MALLORY, A. C. - VAUCHERET, H. 2006. Functions of microRNAs and related small RNAs in plants. In Nature Genetics , vol. 38 , 2006, no. 7, pp. 31-36. NAKASHIMA, T. - SEKIGUCHI, T. - KURAOKA, A. - FUKUSHIMA, K. - SHIBATA, Y. - KOMIYAMA, S. 1993. Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells. In

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Identification of the Herbal Drug Prunellae spica based on Macroscopic and Microscopic Characteristics

-Yeung C, Qing-Feng Z (2008) Enhanced analysis of triterpenes, flavonoids and phenolic compounds in Prunella Vulgaris L. by capillary zone electrophoresis with the addition of running buffer modifiers. J Chromatogr A 1213(2): 231-238. doi: 10.1016/j.chroma.2008.10.033 9. Huynh HT, Teel RW (1999) Selective Induction of Apoptosis in Human Mammary Cancer Cells (MCF-7) by Pycnogenol. Anticancer Res, 1999; 20: 2417-2420. 10. Hwang YJ, Lee EJ, Kim HR, Hwang KA (2013) In vitro antioxidant and anticancer effects of solvent fractions from Prunella vulgaris var

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Therapeutic Aspects of Catechin and Its Derivatives – An Update

(2017) Catechins activate muscle stem cells by Myf5 induction and stimulate muscle regeneration. Biochem Biophys Res Commun. 489(2):142-148. 22. Kimura-Ohba S, Yang Y (2016) Oxidative DNA Damage Mediated by Intranuclear MMP Activity Is Associated with Neuronal Apoptosis in Ischemic Stroke. Oxid. Med. Cell. Longev. http://dx.doi.org/10.1155/2016/6927328 23. Koo SI, Noh SK (2007) Green Tea as Inhibitor of the Intestinal Absorption of Lipids: Potential Mechanism for its Lipid-Lowering Effect. J Nutr Biochem. 18(3):179-183. 24. Li F, Jin H, Xiao J, Yin

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Genetic diversity of Potamogeton pectinatus L. in Iran as revealed by ISSR markers

genetic software for teaching and research. Molecular Ecology Notes 6, 288–295. Ren, D., Peng, G., Huang, H., Wang, H., Zhang, Sh., 2006: Effect of rhodoxanthin from Potamogeton crispus L. on cell apoptosis in Hela cells. Toxicology in Vitro 20, 1411–1418. Sandsten, H., Beklioglu, M., Ince, O., 2005: Effect of waterfowl, large fish and periphyton on the spring growth of Potamogeton pectinatus L. in lake Mogan Turkey. Hydrobiologia 537, 239–5478. Sculthorpe, C. D., 1967: Biology of aquatic vascular plants. Edward Arnold (Publishers) Ltd., London

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Cytochemical and ultrastructural observations of anthers and pollen grains in Lathyrus undulatus Boiss

Cytochemistry 6, 22. Olofsson, M. H., Havelka, A. M., Brnjic, S., Shoshan, M. C., Linder, S., 2008: Charting calcium-regulated apoptosis pathways using chemical biology: role of calmodulin kinase II. BMC Chemical Biology 8, 2. Pacini, E., Franchi, G. G., Hesse, M., 1985: The tapetum: its form, function and possible phylogeny in Embryophyta. Plant Systematics and Evolution 149, 155-185. Pacini, E., 1994: Cell biology of anther and pollen development. In: Williams, E. G., Clarke, A. E., Knox, R. B

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Allium cepa root meristem cells under osmotic (sorbitol) and salt (NaCl) stress in vitro

to drought, salt and freezing, abiotic stresses that affect plant water status. Plant Journal 45, 523–539. West, G., Inze, D., Gerrit, T. S., Beemster, G. T. S., 2004: Cell cycle modulation in the response of the primary root of Arabidopsis to salt stress. Plant Physiology 135, 1050–1058. Yazdani, M., Mahdieh, M., 2012: Salinity induced apoptosis in root meristematic cells of rice. International Journal of Bioscience, Biochemistry and Bioinformatics 2, 40–43. Yi, H., Meng, Z., 2003: Genotoxicity of hydrated sulfur dioxide on root tips of Allium

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Antibacterial, cytotoxic and trypanocidal activities of marine-derived fungi isolated from Philippine macroalgae and seagrasses

., Lee, Y. M., Hong, J., Bae, K. S., Choi, J. S., Jung, J. H., 2011: A new antioxidant from the marine sponge-derived fungus Aspergillus versicolor . Natural Product Sciences 17, 14–18. Li, Y. X., Himaya, S. W. A., Dewapriya, P., Zhang, C., Kim, S. K., 2013: Fumigaclavine C from a marine-derived fungus Aspergillus Fumigatus induces apoptosis in MCF-7 breast cancer cells. Marine Drugs 11, 5063–5086. Lin, A., Lu, X., Fang, Y., Zhu, T., Gu, Q., Zhu, W., 2008: Two new 5-hydroxy-2-pyrone derivatives isolated from a marine-derived fungus Aspergillus flavus

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Sinapinic and protocatechuic acids found in rapeseed: isolation, characterisation and potential benefits for human health as functional food ingredients

genes involved in the cell cycle regulation, cellular differentiation and apoptosis ( Tortorella et al ., 2015 ). Thus, HDAC inhibitors are emerging as potential therapeutic agents. There has been success in developing clinical therapeutics using HDAC inhibitors from both natural and synthetic sources. For example, suberoylanilide hydroxamic acid (SAHA) (vorinostat) and the natural product, romidepsin, were both approved for the treatment of cutaneous T-cell lymphoma ( Mair et al ., 2014 ). SA was previously shown to have HDAC inhibitory activity. Senawong et al

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Garlic Sulfur Compounds Suppress Cancerogenesis and Oxidative Stress: a Review

the proliferation and induces apoptosis of human colon cancer cells through oxidative modification of beta-tubulin. Journal of Biological Chemistry, 280, 41487-41493. doi: 10.1074/jbc. M507127200. Iciek M, Kwiecien I, Wlodek L (2009): Biological properties of garlic and garlic-derived organosulfur compounds. Environmental and Molecular Mutagenesis, 50, 247-265. doi: 10.1002/em.20474. Ide N, Lau BHS (2001): Garlic compounds minimize intracel - lular oxidative stress and inhibit nuclear factor-kappa B activation. Journal of Nutrition

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