Taking Camellia oleifera seeds as raw materials, this study explored extraction and purification of glycoprotein separated from Camellia seeds as well as its antitumor activity, aiming to provide a theoretical basis for the economic development of Camellia oleifera industry. Key impact factors of Camellia seed glycoprotein were extracted using buffer solution method and water extraction method and a regression model was set up. Methyl thiazolyl tetrazolium was used to evaluate the in vitro antitumor activity of glycoprotein extracted from Camellia seeds and Differential Scanning Calorimetry (DSC) was used to measure its denaturation enthalpy value. Results indicated that protein and sugar yields were 8.96% and 17.05% respectively under optimal conditions when water extraction method was used. Crude glycoprotein extracted from Camellia oleifera had a certain inhibitory effect on human hepatoma cell HepG2, gastric cancer cell MGC-803 and breast cancer cell MCF-7 and crude glycoprotein extracted from Camellia oleifera by water-extraction and alcohol-precipitation method had a strong antitumor effect. Crude glycoprotein obtained in the two different ways was capable of scavenging DPPH, •OH and O2g- free radicals and also showed good reducing capacity. DSC measurement results revealed that specific rotation of
was - 32.5. Antitumor experiment in vitro showed that glycoprotein extracted from Camellia seeds in the two different ways had a certain inhibitory effect on HepG2, MGC-803 and MCF-7, which has important theoretical and realistic significances to promoting utilization value of camellia resources, strengthening Camellia oleifera’s comprehensive development and utilization of high added value as well as enriching types and functions of active glycoprotein.
Yunxia He, He Chen, Zhangteng Lei, Jili Cao and Yuan Tan
7. Choi, Y.H., Kong, K.R., Kim, Y.A., et al. (2003). Induction of Bax and activation of caspases during beta-sitosterol-meadiated apoptosis in human colon cancer cells. International Journal of Oncology , 23, 1657-1662.
8. De, J.A., Plat, J., Mensink, R.P. (2003). Metabolic effects of plant sterols and stanols. Journal of Nutritional Biochemistry , 14, 362-369.
9. Dutta, P.C. (2004). Chemistry, analysis, and occurrence of phytosterol oxidation products in food. In P. C. Dutta (Ed.), Phytosterols as functional food components and
36. Srivastava, A., Akoh, C.C., Fischer, J. & Krewer, G. (2007) Effect of anthocyanin fractions from selected cultivars of Georgia-grown blueberries on apoptosis and phase II enzymes. J Agric Food Chem. 55(8):3180-5.
37. Tango, J.S.T. Carvalho, C.R.L. & Soares, N.B. (2004) Physical and chemical characterization of avocado fruits aiming its potencial for oil extraction. Revista Brasileira de Fruticultura, 26, n. 1.
38. Tango, J.S. & Turatti, J.M. (1992) Óleo de abacate. In: TEIXEIRA, C. G. et al. Abacate: cultura, matéria-prima, processamento
role for vesicles in fungal secondary metabolism. Proceedings of the National Academy of Sciences. 106 (46), 19533-19538
17. Chen, J., K. Chen, S. Yuan, X. Peng, J. Fang, F. Wang, H. Cui, Z. Chen, J. Yuan, and Y. Geng. (2013). Effects of aflatoxin B1 on oxidative stress markers and apoptosis of spleens in broilers. Toxicology and industrial health. 0748233713500819
18. Cleveland, T. E., P. F. Dowd, A. E. Desjardins, D. Bhatnagar, and P. J. Cotty. (2003). United States Department of Agriculture - Agricultural Research Service research
mercury. Journal of Trace Elements in Medicine and Biology , 18. (2005) 227–234.
 J. W. Finley, J. G. Penland, Adequacy or deprivation of dietary selenium in healthy men: clinical and psychological findings. The Journal of Trace Elements in Experimental Medicine , 11. (1998) 11–27.
 S. Florian, S. Krehl, M. Loewinger, A. Kipp, A. Banning, S. Esworthy, C. Fong-Fong, R. Brigelius-Flohé, Loss of GPx2 increases apoptosis, mitosis, and GPx1 expression in the intestine of mice. Free Radical Biology and Medicine , 49. (2011) 1694–1702.
 T. E
nosemosis. Apidologie. 2010; 41:375-92.
46. Higes M, Juarranz A, Dias-Almeida J, Lucena S, Botias C, Meana A, Garcia-Palencia P, Martin-Hernandez R. 2013. Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera). Environ. Microbiol. Rep. 5(4), 530-6.
47. Huang WF, Jiang JH, Chen YW, et al. A Nosema ceranae isolate from the honeybee Apis mellifera. Apidologie 2007; 38: 30-7.
48. Huang WF, Solter LF. Comparative development and tissue tropism of Nosema apis and Nosema ceranae