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Investigation of Biofilm Formation and Phylogenetic Typing of Escherichia Coli Strains Isolated from Milk of Cows with Mastitis / Ispitivanje Formiranja Biofilma I Filogenetska Tipizacija Sojeva Escherichia Coli Izolovanih Iz Mleka Krava Sa Mastitisom


Escherichia coli is an opportunistic pathogen affecting bovine mammary gland causing mainly transient infections; however, some recent reports indicated that some strains are able to adhere to and internalize into the epithelial cells, which can result in the persistence of the pathogen in the tissue and development of recurrent mastitis. The mechanism of adaptation of E. coli to the mammary gland relies on structures that are distinctive components of its extracellular matrix - curli fimbriae (proteinaceous component) and cellulose (polysaccharide). Expression of these components varies among the isolates. In this study, we investigated the capacity of expression of curli fimbriae and cellulose (via colony morphotype on Congo Red agar) and ability of biofilm formation (microtiter plate test) in 25 strains of E. coli isolated from milk of cows with clinical mastitis. Phylogenetic grouping of the isolates was performed using PCR method based on detection of chuA, yjaA and TspE4-C2 amplicons. Antimicrobial susceptibility was examined using standard disk diffusion test. Production of both extracellular matrix components was established in 56%, and expression of curli fimbriae in 64% E. coli isolates. All isolates that produced curli fimbriae, demonstrated this ability at a temperature of 37°C, indicating the potential role of these adhesive structures in the pathogenesis of mastitis. The results of phylogenetic typing confirmed that E. coli strains isolated from milk of cows with mastitis are typical commensals mainly belonging to phylogenetic groups A and B1. All curli and curli/cellulose producing isolates formed biofilm under in vitro conditions. The biofilm potentially plays an important role in the development of persistent infections as well as recurrent clinical symptoms after antibiotic therapy in spite of quite good in vitro antimicrobial susceptibility of the agent.

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Polyarthritis in Goat-Kids Caused by Streptococcus Equi Subspecies Zooepidemicus

S, Werle R, Militz M, Bührer V, 2010. Human bacterial arthritis caused by Streptococcus zooepidemicus: report of a case. International Journal of Infectious Diseases 14S e233-e235. 18. Kang M, Ko Y, Liang X, Ross CL, Liu Q, Murray BE, Höök M: Collagen-binding Microbial Surface Components Recognizing Adhesive Matrix Molecule (MSCRAMM) of Grampositive Bacteria Inhibit Complement Activation via the Classical Pathway. J Biol Chem 2013, 288:20520-20531. 19. Visai L, Bozzini S, Raucci G, Toniolo A, Speziale P: Isolation and

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Evaluation of long-term antibody response and cross-serotype reaction in ducks immunised with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN

–511. 10.1586/erv.10.174 21506647 Bode C. Zhao G. Steinhagen F. Kinjo T. Klinman D.M. CpG DNA as a vaccine adjuvant Expert Rev Vaccines 2011 10 499 511 2 Calderon-nieva D.: Improving the delivery and immunogenicity of an inhalable CpG-ODN DNA vaccine by bio-adhesive gemini nanoparticles in neonatal chickens. UWSpace 2018. Calderon-nieva D. Improving the delivery and immunogenicity of an inhalable CpG-ODN DNA vaccine by bio-adhesive gemini nanoparticles in neonatal chickens UWSpace 2018 http

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Pathogenicity of FtsK mutant of avian pathogenic Escherichia coli

References 1. Begg K.J., Dewar S.J., Donachie W.D.: A new Escherichia coli cell division gene, ftsK. J Bacteriol 1995, 177, 6211-6222. 2. Bigot S., Sivanathan V., Possoz C., Barre F.X., Cornet F.: FtsK, a literate chromosome segregation machine. Mol Microbiol 2007, 64, 1434-1441. 3. Chen X., Gao S., Wang X.Q., Jiao X.A., Liu X.F.: Identification of APEC genes expressed in vivo by selective capture of transcribed sequences. Acta Microbiol Sin 2007, 47, 407-412. 4. Dho M., Lafont J.P.: Adhesive

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Comparison of clinical, biomechanical, and histopathological effects of various suture techniques on repair of tendon rupture by using autograft

.M.: Comparative evaluation of the tendon-bone interface contact pressure in different single-versus double-row suture anchor repair tecnhiques. Knee Surg Sports Traumatol Arthosc 2009, 17, 1466–1472. 7. Chan A.P.H., Lo C.K., Lam H.Y., Fung K.Y.: Unusual traumatic triceps tendon avulsion rupture:a word of caution. Hong Kong Med J 2009, 15, 294–296. 8. Cihan M., Özaydın İ.: Experimental tenorraphy with fibrin adhesive (Tiseel) in sheep. Kafkas Üniv Vet Fak Derg 1999, 5, 103–112. 9. Deny H.R.: Surgery of the stifle joint. In: A Guide to Canine Orthopaedic

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Original Scientific Article. Assessment of Reagent Effectiveness and Preservation Methods for Equine Faecal Samples

´Goran, E. K. N., Cringoli, G. (2008). FLOTAC: a new sensitive technique for the diagnosis of hookworm infection in humans. Trans. R. Soc. Trop. Med. Hyg. 102, 84-90. PMid:18028969 4. Jeardon, A., Abdykdaueva, G., Usubalieva, J., Ensink, J. H. J., Cox, J., Matthys, B., Rinaldi, L., Cringoli, G., Utzinger, J.(2010). Accuracy of the Kato-Katz, adhesive tape and FLOTAC techniques for helminth diagnosis among children in Kyrgyzstan. Acta. Trop. 116, 185-192. PMid

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Contribution of Pili of S. Pneumoniae in the Onset of Meningitis

adhesive clusters of Rrg A. PLoS Pathogens , 4, e1000026. 11. Hilleringmann, M., Ringler, P., Müller, S. A., De Angelis, G., Rappuoli, R., Ferlenghi, I., et al., 2009: Molecular architecture of Streptococcus pneumoniae TIGR4 pili. The EMBO Journal , 28, 3921—3930. . 12. Honda, E., Yanagawa, R., 1978: Pili-mediated attachment of Corynebacterium renale to mucous membrane of urinary bladder of mice. Am. J. Vet. Res. , 39, 155—158. 13. Iovino, F., Hammarlöf, D. L., Garriss, G., Brovall, S

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The Effect of Combined Feed Additives on Growing Pigs’ Performance and Digestive Tract Parameters

. Gheisar M.M., Kim I.H. (2018). Phytobiotics in poultry and swine nutrition – a review. It. J. Anim. Sci., 17: 92–99. Grajek K., Sip A., Foksowicz-Flaczyk J., Dobrowolska A., Wita A. (2016). Adhesive and hydrophobic properties of the selected LAB isolated from gastrointestinal tract of farming animals. Acta Bioch. Pol., 63: 311–314. Grashorn M.A. (2010). Use of phytobiotics in broiler nutrition – an alternative to infeed antibiotics? J. Anim. Feed Sci., 19: 338–347. Han G.G., Lee J.Y., Jin G.D., Park J., Choi Y.H., Chae B.J. et al. (2016) Evaluating the

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Nematophagous Activity of Duddingtonia Flagrans MUCL 9827 Against Sheep Gastrointestinal Nematodes

emphasis on Duddingtonia flagrans . Veterinary Research, 33(6): 685-696, 2002. COOKE, R. G.: Two nematode-trapping hyphomycetes, Duddingtonia flagrans gen. et comb. nov. and Monagrosporium mutabilis sp. nov. Transactions of the British Mycological Society, 53(2): 315-319, 1969. CVETKOVIĆ, LJ.: Hemioprofilaksa značajnijih pašnih helmintoza preživara. Praxis Veterinaria 24(1):23-31, 1976. (in Serbian) DEN BELDER, E., JANSEN, E.: Capture of plant-parasitic nematodes by an adhesive hyphae forming isolate of Arthrobotrys oligospora and some other

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