Marta Masternak, Joanna Knap and Krzysztof Giannopoulos
melanoma and glioblastoma [ 2 , 3 , 4 , 5 , 6 , 7 ]
Therefore, other platelet indices, including MPV, were considered as potential prognosis-affecting factors. MPV is a machine-calculated measurement of the average size of platelets found in the blood. It reflects the platelet production in bone marrow or platelet destruction problems [ 8 ]. Recent studies revealed a significant impact of MPV on the course and prediction in diferent types of neoplasms. This is particularly interesting in the context of the potential use of a relatively cheap, high reproducible
Agnieszka Szymczyk, Aleksandra Nowaczyńska, Maciej Korpysz, Helena Donica, Agnieszka Bojarska-Junak, Monika Długosz-Danecka, Waldemar Tomczak, Ewa Wąsik-Szczepanek and Iwona Hus
. Clin Chem Lab Med 2016;54:1073–83.
Barnidge DR Dispenzieri A Merlini G et al Monitoring free light chains in serum using mass spectrometry Clin Chem Lab Med 2016 54 1073 – 83
 Hansen CT. Performance goals for immunoglobulins and serum free light chain measurements in plasma cell dyscrasias can be based on biological variation. Clin Chem Lab Med 2016;54:1031–3.
Hansen CT Performance goals for immunoglobulins and serum free light chain measurements in plasma cell dyscrasias can be based on biological variation Clin Chem Lab Med 2016 54
Monika Adamska, Anna Komosa, Tatiana Mularek, Joanna Rupa-Matysek and Lidia Gil
tissue biopsies are not available. This is a gold standard diagnostic tool directly proving the presence of amyloid infiltration; however, it should be performed only in specialized centers by experienced operators [ 19 ]. EMB is an invasive procedure, with the risk of serious complications. During the procedure, heart catheterization is performed, enriching the operator information from hemodynamic measurements [ 4 ]. Right ventricular EMB is routinely performed; however, recently, LV EMB appears diagnostically more contributive [ 20 , 21 ]. The sensitivity of
Bembnista Ewa, Kubiak Agnieszka, Matuszak Paula and Kozłowska-Skrzypczak Maria
Transfusion 2002 42 10 17
 Arlet G, Gluckman E, Gerber F, Perol Y, Hirsch A. Measurement of bacterial and fungal air counts in two bone marrow transplant units. J Hosp Infect, 1989; 13: 63-69 10.1016/0195-6701(89)90096-0 Arlet G Gluckman E Gerber F Perol Y Hirsch A. Measurement of bacterial and fungal air counts in two bone marrow transplant units J Hosp Infect 1989 13 63 69
 Kaiser K, Wolski A. Kontrola czystości mikrobiologicznej powietrza. Technika chłodnicza i klimatyzacyjna, 2007; 4: 158-162 Kaiser K Wolski A. Kontrola czystości mikrobiologicznej powietrza
Agnieszka Pluta, Tadeusz Robak, Kamil Brzozowski, Barbara Cebula-Obrzut, Agata Majchrzak, Piotr Pluta, Anna Szmigielska-Kapłon, Olga Grzybowska-Izydorczyk, Magdalena Czemerska, Piotr Stelmach, Piotr Smolewski and Agnieszka Wierzbowska
described in detail [ 16 , 30 ].
Flow cytometry analysis
All fluorescence measurements were performed by the use of flow cytometry (FACScan; Becton-Dickinson, San Jose, CA, USA). An acquisition gate was established based on forward scatter (FSC) and side scatter (SSC) that included mononuclear cells according to the previous immunophenotype. Cell fluorescence was measured using standard emission filters: FL1 (green, λ 515–545 nm) and FL2 (orange, λ 564–606 nm). For each analysis, 10,000 events were acquired and analyzed using CellQuestPro software (Becton
-inflammatory treatment, used systemic corticosteroids, and had systemic inflammatory disease were excluded from the study.
The severity of pain was assessed with the visual analog scale (VAS). For using VAS, patients are asked to rate the severity of their headaches on a 10 cm scale varying from zero (no pain) to 10 (the worst pain imaginable).
For the study, blood samples were taken from arm veins in tubes containing ethylenediamine tetraacetic acid (EDTA) and stored at room temperature. Samples were studied within 2 hours. The Plt number was observed using a
The two major autoimmune diseases demonstrating activated partial thromboplastin time (APTT) prolongation and normal prothrombin time (PT) on coagulation screening are antiphospholipid (aPL) antibody syndrome (APS) and acquired hemophilia. APTT cross-mixing test demonstrates an inhibitory pattern in both diseases, and the diagnoses are made according to clinical findings and the measurement of aPL antibodies or coagulation factor activities. Several cases of coexistent APS and acquired hemophilia have been reported; however, the mechanisms
Edyta Klimczak-Jajor, Joanna Skulimowska, Anna Ejduk, Katarzyna Guz, Małgorzata Uhrynowska and Ewa Brojer
blood cell microcytosis with no iron deficiency in the outpatient clinic of Institute of Hematology and Transfusion Medicine in Warsaw. Complete blood counts on Cell Dyn 4000 automated analyzer was performed within 24 hours of sample collection.
Biochemical analysis included microcolumn chromatography for quantization of HbA 2 (Beta-Thal HbA 2 Quick Column (Helena Biosciences)) and alkaline denaturation procedure for measurement of HbF and alkaline and acid hemoglobin electrophoresis (Sebia) to detect pathological hemoglobin fraction.
Genomic DNA was extracted
Samia Abd El-Moneim Ebied, Nadia Aly Sadek, Nadia El-Sayed Zaki, Samir Ali Abd El- Kaream and Heba Khafagui Ahmed El Kashif
Basic haematological techniques In: Practical haematology 13th (ed) Edingburgh, London, Melbourne, New York. Churchill Livingstone 2009 3 19 – 46
 Leng S, McElhaney J, Walston J, Xie D, Fedarko N, Kuchel G. Elisa and multiplex technologies for cytokine measurement in inflammation and aging research. J Gerontol A Biol Sci Med Sci 2008;63:879–84. 10.1093/gerona/63.8.879
Leng S McElhaney J Walston J Xie D Fedarko N Kuchel G Elisa and multiplex technologies for cytokine measurement in inflammation and aging research J Gerontol A Biol
lines HL60, ML-1, RAji, and Jurkat. The authors found through measurements of the half-maximal inhibitory concentration (IC 50 ) that the combination of DAC and Ara-C showed an additive or synergistic induction of cell death in all cell lines. In addition, a sequential schedule with DAC followed by Ara-C was valid.
Granulocyte colony-stimulating factor (G-CSF) priming induced G0/ G1 phase leukemia cells into an S phase, enhancing cell response to DAC. Then, DAC (15 mg/m 2 D1–5) plus CAG (termed D-CAG, comprising Ara-C [10 mg/m 2 , q12h, D3–9], aclarubicin [10 mg