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The integration of the molecular methods in the diagnosis algorithm for the poliovirus detection in the sewage water: comparing concentration and detection methods. A Pilot Study

Abstract

Introduction. Two cases of circulating vaccine-derived poliovirus type 1(cVDPV1), from southwestern Ukraine, bordering Romania, were confirmed in 2015 and the environmental enterovirus surveillance was enhanced in our country. The molecular detection of human enteroviruses as a screening test followed by isolation on cell culture lines or sequencing could be proposed as a new diagnosis algorithm.

Material and Methods. The sensitivity of two molecular methods for the detection of enterovirus strains in 10 mL of sewage water (15 samples) was studied with Film Array ME panel BioFire (Biomerieux, France) and Xpert EV assay (Cepheid, USA). These are standardized methods for the detection of microorganisms in the cerebrospinal fluid (CSF).

Results. Of the 15 samples, six enterovirus strains were detected using Film Array ME, four enterovirus strains were detected using Xpert EV assay, while only two nonpolio enterovirus strains were isolated on RD cell line, using the standard WHO algorithm. However, only one of the strains detected by the standard WHO algorithm was detected by one of the molecular methods.

Conclusions. The molecular methods for enterovirus detection are more sensitive than the virus isolation on cell culture lines, but in one case the virus isolated on RD cell line was not detected by the molecular methods. The results could be influenced by the small number of the samples investigated, by the volume and the concentration method used for samples tested, and by the limits of detection (LoD) of the enterovirus species in the samples, depending on the method used.

Open access
Psychological factors and psychosocial interventions for cancer related pain

., VISSERS K., ENGELS Y. Patient empowerment in cancer pain management: an integrative literature review. Psycho-Oncol. 2014; 23 (11):1203–11.

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Practical Aspects Regarding the Histopathological Diagnosis of Early Mycosis Fungoides

Mycosis fungoides is the most common primary T-cell lymphoma of skin. The disease has a protean clinical and histological presentation in its early patch and plaque stages, when distinction from mimicking inflammatory dermatoses is difficult. Since no single criterion is specific enough, a reliable diagnosis in early stages requires integration of clinical, histopathological and molecular findings. In skin biopsies, the most helpful histologic features are the detection of atypical lymphocytes in the epidermis with minimal epidermal changes, basal alignment of lymphocytes along dermal-epidermal junction and formation of Pautrier microabscesses. An aberrant immunophenotype of T cells and molecular detection of a clonal T-cell population are factors that could allow a more specific diagnosis. This work recapitulates and discusses these features from a practical perspective.

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Cardiac sarcoidosis: Case presentation and Review of the literature

Abstract

Cardiac sarcoidosis usually occurs in the context of systemic disease; however, isolated cardiac involvement can occur in up to 25% of cases and tends to be clinically silent. When symptoms are present, they are often nonspecific and occasionally fatal, representing a diagnostic challenge. A high index of clinical suspicion and the integration of appropriate imaging, laboratory, and pathologic findings is always required. Treatment aims to control the systemic inflammatory condition while preventing further cardiac damage. However, even with adequate diagnosis and treatment strategies, prognosis remains poor. We describe the case of a patient who presented with cardiac symptoms, whose initial examination was unrevealing. Diagnosis was made retrospectively based on later systemic manifestations that revealed characteristic sarcoidosis findings.

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Congenital Pseudohypoparathyroidism – A Late Diagnosis

Abstract

The purpose of this paper is to present a case of congenital pseudohypoparathyroidism, late diagnosed in a 22-year-old patient.

The patient’s history revealed hypocalcaemia, diagnosed at birth and persistent despite the treatment with calcium. At 8 years old, the patient is diagnosed with epilepsy and receives treatment with Levetiracetam and Oxcarbazepine; at 12 years old she is diagnosed with dilatative cardiomyopathy and receives treatment with Spironolactone and Glycosides. At 22 years old, she visits our Internal Medicine Department with the suspicion of polymyositis and psoriasis. Clinical examination shows armonic short stature, fourth finger hypoplasia, laboratory findings show severe hypocalcaemia, the hand X-ray - third and fourth metacarpal hypoplasia, immunological tests were negative. All data leads to the diagnosis of congenital disease, and given the history of the patient and the evolution of the clinical manifestations we presume hypoparathyroidism or pseudohypoparathyroidism, therefore PTH is dosed – with normal values, and the diagnosis of congenital pseudohypoparathyroidism is established. The patient was referred to endocrinology, where genetic tests were performed to confirm the diagnosis.

In conclusion, in the absence of multiple pathology integration into a single disease, the diagnosis of the genetic disease is delayed. Therefore, it is important to have a comprehensive approach and collaboration between different specialties to establish the correct diagnosis.

Open access
Family Quality of Life Among Families with a Member Diagnosed with CLD – What to Expect?

epidemiological data. Journal of Hepatology 2013, vol. 58, issue 3, 593-608. 5. Fitzpatrick R, Davey C, Buxton MJ, Jones DR. Evaluating patient based outcome measures for use in clinical trials. Health Technol Assess. 1998;2:i–iv. 1–74. 6. Costanza R, Fisher B, Ali A, et al. An integrative approach to quality of life measurement, research, and policy, S.A.P.I.EN.S, 1.1 | 2008, (Online), Online since 19 décembre 2008. URL: http://sapiens.revues.org/index169.html . Connection on 03 août 2010. 7. Werner, S., Edwards, M., Baum, N., Brown, I., Brown, R. I. and

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Macrophage activation syndrome at the onset of glucocorticoid-resistant systemic lupus erythematosus: a case report

American journal of medicine. 2014; 127 (11):1118-25. 9. TAKAHASHI H, TSUBOI H, KURATA I, TAKAHASHI H, INOUE S, EBE H, et al. Predictors of the response to treatment in acute lupus hemophagocytic syndrome. Lupus. 2015; 24 (7):659-68. 10. INGAWALE DK, MANDLIK SK, PATEL SS. An emphasis on molecular mechanisms of anti-inflammatory effects and glucocorticoid resistance. Journal of complementary & integrative medicine. 2015; 12 (1):1-13. 11. ZHANG B, SHI Y, LEI TC. Detection of active P-glycoprotein in systemic lupus erythematosus patients with poor

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Profiles of peptide YY and ghrelin, levels of hunger and satiety, and ad libitum intake in obese and non-obese Indonesian women

loss maintenance. In: Kopelman PG, Caterson ID, Dietz WH, editors. Clinical obesity in Adult and Children . 2 ed. Massachusetts: Blackwell Publishing; 2005:407-20. 4. MURPHY KG., BLOOM SR. Gut hormones in the control of appetite . Exp Physiol. 2004; 89 (5):507-16. 5. RAYBOULD HE., MEYER JH., TABRIZI Y., RODGER A., TSO L., TSO P. Inhibition of gastric emptying in response to intestinal lipid is dependent on chylomicron formation . Am J Physiol Regulatory Integrative Comp Physiol. 1998; 274 :1834-8. 6. DULLOO AG. Energy balance and body weight

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Metabolic and bone profile in postmenopausal women with and without type 2 diabetes: a cross-sectional study

al. Insulin signaling in osteoblasts integrates bone remodeling and energy metabolism . Cell. 2010; 142 (2): 296-308. 5. MATTHEW P. GILBERT, RICHARD E. PRATLEY. The impact of diabetes and diabetes medications on bone health . Endocrine Reviews, (2015); 36 (2):194–213. 6. ZOCH ML, CLEMENS TL, RIDDLE RC. New insights into the biology of osteocalcin . Bone. 2016; 82 :42-49. 7. FAIENZA MF, LUCE V, VENTURA A, et al. Skeleton and glucose metabolism: a bone-pancreas loop . International Journal of Endocrinology (2015); 2015 :758148. 8. SHAO J

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