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Ultrastructure of adhesive bond of composite to dentin

quality of bonded resin composite restorations. Dent Mater 2005; 21:397-412. http://dx.doi.org/10.1016/j.dental.2004.07.005 10. Drobac Milan. Bond strength evaluation and micromorphological characterization of caries affected dentin/ resin interfaces. Dissertation. Novi Sad, 2011. 11. LuoY, Lo ECM,Wei SHY, Tay FR. Comparison of pulse activation vs conventional light-curing on marginal adaptation of a compomer conditioned using a total-etch or a self- etch technique. Dental Materials 2002; 18:36-48. http://dx.doi.org/10.1016/S0109

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The Effect of Polymerization Technique on Marginal Index of Composite Fillings in Dentin

Dent 2003;31(3):189-96. http://dx.doi.org/10.1016/S0300-5712(03)00015-0 15. Watts DC, Al-Hindi A. Intrinsic soft start” polymerization shrinkage-kinetics in an acrylate-based resin-composite. Dent Mater 1999;15(1):39-45. http://dx.doi.org/10.1016/S0109-5641(99)00012-3 16. Margvelashvili M, Goracci C, Beloica M, Papacchini F, Ferrari M. In vitro evaluation of bondingeffectiveness to dentin of all-in-one adhesives. J Dent 2010;38(2):106-12. http://dx.doi.org/10.1016/j.jdent.2009.09.008 17. Luo Y, Tay FR, Lo ECM, Wei SHY. Marginal adaptation of a

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Relationship between Lipid Indices, Type IV Collagen Turnover and the Development of Microvascular Complications in Diabetic Patients with Arterial Hypertension

IMAB 2012; 18(3): 315-22. 16. Nicoloff G, Deliiyski T, Nikolov A. Detection of serum collagen type IV and elastin derived peptides in patients with breast cancer. Diabetologia Croatica 2010; 39(3): 83-93. 17. Kanie K, Narita Y, Zhao Y, et al. Collagen type IV-specific tripeptides for selective adhesion of endothelial and smooth muscle cells. Biotechnol Bioeng 2012; 109(7): 1808-16. 18. Menon M, Patel D, Taite LJ. Self-assembly of elastin-based peptides into the ECM: the importance of integrins and the elastin binding protein in elastic fiber

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Attitudes of Students from the High Medical College of Professional Studies and Nurses Towards People Suffering from Dementia

ECM. 14. Prince, M., Ali, G.C., Guerchet, M., Prina, A.M., Albanese, E. & Wu, Y.T. (2016). Recent global trends in the prevalence and incidence of dementia, and survival with dementia. Alzheimers Res Ther. 8:23.DOI:10.1186/s13195-016-0188-8. 15. Norbergh, K.G., Helin, Y., Dahl, A., Hellzén, O. & Asplund, K. (2006). Nurses’ attitudes towards people with dementia: the semantic differential technique. Nurs Ethics. 13:264-74. DOI:10.1191/0969733006ne863oa. 16. Yong, M.H., Yoo, C.U. & Yang, Y.A. (2015). Comparison of knowledge of and attitudes toward

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Manufacturing of Biodegradable Scaffolds to Engineer Artificial Blood Vessel

ABBREVIATIONS DMF - N,N – dimethylformamide ECs – endothelial cells ECM – extracellular matrix PCL – polycaprolactone PEG – polyethyleneglycol SEM – scanning electron microscopy SMCs – smooth muscle cells REFERENCES 1. Tu JV, Pashos CL, Naylor CD, et al. (1997). Use of cardiac procedures and outcomes in elderly patients with myocardial infarction in the United States and Canada. N. Engl. J. Med . 336(21), 1500–1505. DOI:10.1056/NEJM199705223362106 2. Wang X, Lin P, Yao Q, Chen C. (2007) Development of Small

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The history of public health use of fluorides in caries prevention

Pediatrics 2014 13 1102 11 42 Wong MCM, Clarkson J, Glenny A-M, Lo ECM, Marinho VCC, Tsang BWK et al. Cochrane reviews on the benefits/risks of fluoride toothpastes J Dent Res 2011; 90(Suppl 5): 573-9. Wong MCM Clarkson J Glenny A-M Lo ECM Marinho VCC Tsang BWK et al. Cochrane reviews on the benefits/risks of fluoride toothpastes J Dent Res 2011 90(Suppl 5) 573 9 43 Walsh T, Worthington HV, Glenny A-M, Appelbe P, Marinho VCC, Shi X. Fluoride toothpastes of different concentrations for preventing dental caries in children and adolescents

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Cytokine CCL5 and receptor CCR5 axis in glioblastoma multiforme

types in the GB stroma are affected: the tumour is able to stimulate angiogenesis and co-opt existing vasculature, suppress immune cell functions, disarm microglia and macrophages that should recognize and fight these “foreign elements” in the brain and coerce astrocytes into supporting tumour modification extracellular matrix (ECM) to facilitate invasion. GB cells recruit innate immune cells and change their phenotype to support tumour growth and suppress adaptive immune responses. 14 The increasing understanding of how T cells access the brain and how the tumour

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Hyaluronan and synovial joint: function, distribution and healing

). Follicular-fl uid contents of hyaluronic-acid, follicle-stimulating-hormone and steroids relative to the success of in-vitro fertilization of human oocytes. Fertil Steril 62 : 347-352. Surendrakumar K, Martyn GP, Hodgers ECM. (2003). Sustained release of insulin from sodium hyaluronate based dry powder formulations after pulmonary delivery to beagle dogs. J Control Release 91 : 385-394. Surini S, Akiyama H, Morishita M. (2003). Polyion complex of chitosan and sodium hyaluronate as an implant device for insulin delivery. STP Pharm Sci

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Matrix Metalloproteinase-2 as a Marker of Microvascular Complications in Children and Adolescents with Type 1 Diabetes Mellitus

Matrix Metalloproteinase-2 as a Marker of Microvascular Complications in Children and Adolescents with Type 1 Diabetes Mellitus

Background. Patients with type 1 diabetes develop microangiopathic complications, which are responsible for morbidity in adulthood. It had been widely known that diabetes is associated with alteration in extracellular matrix (ECM) synthesis. Matrix metalloproteinases (MMPs) can potentially contribute to many microvascular and macrovascular complications of diabetes. MMP-2 is responsible for ECM breakdown and their abnormal circulating levels may pre-date clinical evidence of diabetic angiopathy.

Aim of the work. Is to detect the plasma level and activity of matrix metalloproteinase-2, as a serum marker for diabetic vascular diseases, in type 1 diabetic children and adolescents and to identify its relation to the parameters of metabolic control and microvascular complications.

Design. Cross section study including sixty children and adolescents with type 1 diabetes mellitus with age range from 7 to 18 years. They were divided into two groups according to diabetic duration. Group I: Thirty patients with diabetes duration less than 5 years ranging from 8 months to 3 years with a mean duration of 1.66 ± 0.75 years. Group II: Thirty patients with diabetes duration more than 5 years ranging from 5 to 14 years with a mean duration of 7.7 ± 2.84 years. Thirty healthy children and adolescents of comparable age, served as controls. Diabetic patients were also divided according to microvascular complications into complicated (n=27) and non complicated (n=33). All patients and controls were subjected to history taking, thorough clinical examination and laboratory investigations included; random blood sugar, glycosylated hemoglobin (HbA1c), quantitative determination of urinary microalbumin, fundus examination and measuring of plasma matrix metalloproteinase-2 levels (using ELISA) and activity.

Results. MMP-2 levels were significantly higher in diabetic patients with disease duration more than 5 years compared to diabetic patients with disease duration less than 5 years and controls (848.96 ± 96.81, 559.56 ± 41.02 and 224.6 ± 28.4 ng/ml; P< 0.001 respectively). Highly significant increase in MMP-2 levels in complicated compared to non-complicated diabetic patients (p<0.001). Positive significant correlation was found between MMP-2 levels and age, disease duration, random blood glucose and HbA1c in diabetic patients with disease duration > 5 years (r=0.37, 0.43, 0.3, 0.49 respectively, p<0.05).

Conclusion. MMP-2 concentrations are increased in a limited number of young diabetic subjects with complications, elevated MMP-2 levels and activity could be useful as a screening marker for early detection of diabetic microvascular complications and were correlated with parameters of metabolic control and disease duration and their levels may pre-date clinical evidence of diabetic angiopathy.

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In Vitro Characterization of Stem Cells from Human Exfoliated Deciduous Teeth (SHED)

Abstract

Stem cells from human exfoliated deciduous teeth (SHED) provide a new attractive source for stem cells; in this study we further characterize SHED. SHED were isolated, differentiated using osteogenic/ odontogenic differentiation media, characterized using light microscope, SEM and immunocytochemistry using CD44. Also, Immunohistochemistry using CD44 was performed on extirpated pulp tissues. We found that a naturally exfoliated human tooth contains a population of stem cells that attain morphological homogeneity after the first passage, on adding the osteogenic/odontogenic medium, sporadic noduleshaped structures were observed after two weeks that were positively stained with alizarin red and von Kossa stains. SHED stained with H& E showed a basophilic, eccentric nucleus with an eosinophilic cytoplasm in which two differently stained areas were clearly distinguishable. Also we found using SEM that SHED spread on the UBM scaffolds surfaces showing multiple filopodia and formed collagen-like structures by the seventh day. After three weeks, seeded scaffolds incubated in osteogenic/odontogenic media showed many mineralized nodules in the ECM. Cultured SHED revealed positive immunoreactivity when treated with CD44. Also, sections of pulp tissue treated with CD44 depict positively stained cells situated mainly in the perivascular areas reinforcing the hypothesis that pericytes may be the origin of SHED.

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