Stefan Dačić, Aleksandar Mitić, Jelena Popović, Dragica Simonović Dačić and Marko Igić
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Stefan Dačić, Aleksandar Mitić, Marija Nikolić, Milica Cenić, Nenad Stošić and Dragica Dačić-Simonović
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Gordana Stanic, Valentina Opancina, Nemanja Rancic, Jelena Jovic and Dragana Ignjatovic-Ristic
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Maja Milosevic, Nikola Mijailovic, Dalibor Nikolic, Nenad Filipovic, Aleksandar Peulic, Mirko Rosic and Suzana Pantovic
DMF - N,N – dimethylformamide
ECs – endothelial cells
ECM – extracellular matrix
PCL – polycaprolactone
PEG – polyethyleneglycol
SEM – scanning electron microscopy
SMCs – smooth muscle cells
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Tea Šket, Andreja Kukec, Rok Kosem and Barbara Artnik
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Miha Koprivnikar Kranjc, Metka Novak, Richard G. Pestell and Tamara T. Lah
types in the GB stroma are affected: the tumour is able to stimulate angiogenesis and co-opt existing vasculature, suppress immune cell functions, disarm microglia and macrophages that should recognize and fight these “foreign elements” in the brain and coerce astrocytes into supporting tumour modification extracellular matrix (ECM) to facilitate invasion. GB cells recruit innate immune cells and change their phenotype to support tumour growth and suppress adaptive immune responses. 14 The increasing understanding of how T cells access the brain and how the tumour
). Follicular-fl uid contents of hyaluronic-acid, follicle-stimulating-hormone and steroids relative to the success of in-vitro fertilization of human oocytes. Fertil Steril 62 : 347-352.
Surendrakumar K, Martyn GP, Hodgers ECM. (2003). Sustained release of insulin from sodium hyaluronate based dry powder formulations after pulmonary delivery to beagle dogs. J Control Release 91 : 385-394.
Surini S, Akiyama H, Morishita M. (2003). Polyion complex of chitosan and sodium hyaluronate as an implant device for insulin delivery. STP Pharm Sci
Mai Yossef, Hoda Megahed, Sawsan Tawfik, Hanaa El Sherif, Ola Eldin, Manal Mohsen, Nagham El-Beblawy and Amira Adly
Matrix Metalloproteinase-2 as a Marker of Microvascular Complications in Children and Adolescents with Type 1 Diabetes Mellitus
Background. Patients with type 1 diabetes develop microangiopathic complications, which are responsible for morbidity in adulthood. It had been widely known that diabetes is associated with alteration in extracellular matrix (ECM) synthesis. Matrix metalloproteinases (MMPs) can potentially contribute to many microvascular and macrovascular complications of diabetes. MMP-2 is responsible for ECM breakdown and their abnormal circulating levels may pre-date clinical evidence of diabetic angiopathy.
Aim of the work. Is to detect the plasma level and activity of matrix metalloproteinase-2, as a serum marker for diabetic vascular diseases, in type 1 diabetic children and adolescents and to identify its relation to the parameters of metabolic control and microvascular complications.
Design. Cross section study including sixty children and adolescents with type 1 diabetes mellitus with age range from 7 to 18 years. They were divided into two groups according to diabetic duration. Group I: Thirty patients with diabetes duration less than 5 years ranging from 8 months to 3 years with a mean duration of 1.66 ± 0.75 years. Group II: Thirty patients with diabetes duration more than 5 years ranging from 5 to 14 years with a mean duration of 7.7 ± 2.84 years. Thirty healthy children and adolescents of comparable age, served as controls. Diabetic patients were also divided according to microvascular complications into complicated (n=27) and non complicated (n=33). All patients and controls were subjected to history taking, thorough clinical examination and laboratory investigations included; random blood sugar, glycosylated hemoglobin (HbA1c), quantitative determination of urinary microalbumin, fundus examination and measuring of plasma matrix metalloproteinase-2 levels (using ELISA) and activity.
Results. MMP-2 levels were significantly higher in diabetic patients with disease duration more than 5 years compared to diabetic patients with disease duration less than 5 years and controls (848.96 ± 96.81, 559.56 ± 41.02 and 224.6 ± 28.4 ng/ml; P< 0.001 respectively). Highly significant increase in MMP-2 levels in complicated compared to non-complicated diabetic patients (p<0.001). Positive significant correlation was found between MMP-2 levels and age, disease duration, random blood glucose and HbA1c in diabetic patients with disease duration > 5 years (r=0.37, 0.43, 0.3, 0.49 respectively, p<0.05).
Conclusion. MMP-2 concentrations are increased in a limited number of young diabetic subjects with complications, elevated MMP-2 levels and activity could be useful as a screening marker for early detection of diabetic microvascular complications and were correlated with parameters of metabolic control and disease duration and their levels may pre-date clinical evidence of diabetic angiopathy.
Stem cells from human exfoliated deciduous teeth (SHED) provide a new attractive source for stem cells; in this study we further characterize SHED. SHED were isolated, differentiated using osteogenic/ odontogenic differentiation media, characterized using light microscope, SEM and immunocytochemistry using CD44. Also, Immunohistochemistry using CD44 was performed on extirpated pulp tissues. We found that a naturally exfoliated human tooth contains a population of stem cells that attain morphological homogeneity after the first passage, on adding the osteogenic/odontogenic medium, sporadic noduleshaped structures were observed after two weeks that were positively stained with alizarin red and von Kossa stains. SHED stained with H& E showed a basophilic, eccentric nucleus with an eosinophilic cytoplasm in which two differently stained areas were clearly distinguishable. Also we found using SEM that SHED spread on the UBM scaffolds surfaces showing multiple filopodia and formed collagen-like structures by the seventh day. After three weeks, seeded scaffolds incubated in osteogenic/odontogenic media showed many mineralized nodules in the ECM. Cultured SHED revealed positive immunoreactivity when treated with CD44. Also, sections of pulp tissue treated with CD44 depict positively stained cells situated mainly in the perivascular areas reinforcing the hypothesis that pericytes may be the origin of SHED.