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Effects of Cell Seeding Methods on Chondrogenic Differentiation of Rat Mesenchymal Stem Cells in Polyhydroxybutyrate/Chitosan Scaffolds

Abstract

The aim of our study was to examine the effects of passive and active cell seeding techniques on in vitro chondrogenic differentiation of mesenchymal stem cells (MSC) isolated from rat bone marrow and seeded on porous biopolymer scaffolds based on polyhydroxybutyrate/chitosan (PCH) blends. This paper is focused on the distribution of the cells on and in the scaffolds, since it influences the uniformity of the created extracellular matrix (ECM), as well as the homogenity of the distribution of chondrogenic markers in vitro which ultimately affects the quality of the newly created tissue after in vivo implantation. The three types of cell-scaffold constructs were examined by: fluorescence microscopy, SEM, histology and quantitative analysis of the glycosaminoglycans after chondrogenic cultivation. The results demonstrated that the active cells seeded via the centrifugation of the cell suspension onto the scaffold guaranteed an even distribution of cells on the bulk of the scaffold and the uniform secretion of the ECM products by the differentiated cells.

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Extracellular matrix in tumours as a source of additional neoplastic lesions - a review

Abstract

The review describes the role of cells of extracellular matrix (ECM) as a source of neoplastic outgrowths additional to the original tumour. The cells undergo a spontaneous transformation or stimulation by the original tumour through intercellular signals, e.g. through Shh protein (sonic hedgehog). Additionally, cells of an inflammatory infiltrate, which frequently accompany malignant tumours and particularly carcinomas, may regulate tumour cell behaviour. This is either by restricting tumour proliferation or, inversely, by induction and stimulation of the proliferation of another tumour cell type, e.g. mesenchymal cells. The latter type of tumour may involve formation of histologically differentiated stromal tumours (GIST), which probably originate from interstitial cells of Cajal in the alimentary tract. Occasionally, e.g. in gastric carcinoma, proliferation involves lymphoid follicles and lymphocytes of GALT (gut-associated lymphoid tissue), which gives rise to lymphoma. The process is preceded by the earlier stage of intestinal metaplasia, or is induced by gastritis alone. This is an example of primary involvement of inflammatory infiltrate cells in neoplastic progression. Despite the numerous histogenetic classifications of tumours (zygotoma benignum et zygotoma malignum, or mesenchymomata maligna et mesenchymomata benigna), currently in oncological diagnosis the view prevails that the direction of tumour differentiation and its degree of histologic malignancy (grading) are more important factors than the histogenesis of the tumour.

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Current clinical applications of adipose-derived stem cells in humans and animals

morphogenetic protein-2. Eur Cells Mater. 2012;24:308–19; DOI:10.22203/eCM.v024a22. 10.22203/eCM.v024a22 Mehrkens A Saxer F Güven S Hoffmann W Müller AM Jakob M Weber FE Martin I Scherberich A Intraoperative engineering of osteogenic grafts combining freshly harvested, human adipose-derived cells and physiological doses of bone morphogenetic protein-2 Eur Cells Mater 2012 24 308 19 10.22203/eCM.v024a22 27 Thesleff T, Lehtimäki K, Niskakangas T, Mannerström B, Miettinen S, Suuronen R, Öhman J. Cranioplasty with adipose

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The role of uterine microbiome and epithelial-mesenchymal transition in endometrial function

matrix (ECM) and formation of intercellular junctions. The mesenchymal cells have typical elongated spindle shape, vimentin expression and ability to move in the ECM using pseudopodia of the plasma membrane. The specific cellular markers are presented in an overview in table 2 . The process of EMT and MET is defined as ability of cells to change the characteristic features of one cellular type to the other type. It is important to realize that this process is gradual in a certain period of time. During EMT/MET, cells of a ”hybrid phenotype“ expressing features typical

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Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products

aim of this study was to investigate the reduction products of selected azo dyes using the technique of electrochemistry with mass spectrometry (EC–MS). Afterwards, a comparison was made with data available in literature and software for predicting metabolic pathways. Particular attention was paid to the harmful aromatic amine reduction EC products. Based on these results an analytical method using liquid chromatography combined with tandem mass spectrometry (LC–MS/MS) was developed as a tool for the confirmation of the identified products obtained by

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Effects of ketosis in dairy cows on blood biochemical parameters, milk yield and composition, and digestive capacity

(Foss, Denmark). The daily milk yield for each cow was recorded and converted into the energy-corrected milk (ECM) yield based on the fat and protein contents of the milk, as follows: ECM (kg/cow per day) = 0.25 × mass of milk yield in kg + 12.2 × mass of fat yield in kg + 7.7 × mass of protein yield in kg. The ECM formula was given by Bell et al . ( 1 ) and cited by Sjaunja et al . ( 22 ). The TMR refused by each cow was collected and weighed daily before the morning feed. Refused feed was sampled on a daily basis and subjected to immediate DM analysis (105°C for

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The Distribution of Collagen and Elastic Fibres in the Lactating Bovine Mammary Gland

: Mammary gland ECM remodeling, stiffness, and mechanosignaling in normal development and tumor progression. Cold Spring Harbor Perspectives in Biology , 3, 1. DOI: 10.1101/cshperspect.a003228. 18. Schedin, P., O’Brien, J., Rudolph, M., Stein, T., Borges, V., 2007: Microenvironment of the involuting mammary gland mediates mammary cancer progression. J. Mammary Gland Biol. Neoplasia , 12, 71—82. DOI:10.1007/s10911-007-9039-3. 19. Tamburro, A. M., De Stradis, A., D’Alessio, L., 1995: Fractal aspects of elastin supramolecular organization. J. Biomol. Struct

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Myeloperoxidase: New Roles for an Old Molecule

, Castro L, Figueroa M, Chumley P, et al. Endothelial transcytosis of myeloperoxidase confers specificity to vascular ECM proteins as targets of tyrosine nitration. J Clin Invest 2001; 108: 1759-70. Sugiyama S, Kugiyama K, Aikawa M, Nakamura S, Ogawa H, Libby P. Hypochlorous acid, a macrophage product, induces endothelial apoptosis and tissue factor expression: involvement of myeloperoxidase-mediated oxidant in plaque erosion and thrombogenesis. Arterioscler Thromb Vasc Biol 2004; 24: 1309-14. Meuwese MC, Stroes ES

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The Relationship of Myocardial Collagen Metabolism and Reverse Remodeling after Cardiac Resynchronization Therapy

List of abbreviations: CRT, cardiac resynchronization therapy PINP, amino-terminal propeptide type I CITP, carboxy-terminal collagen telopeptide ECM, extracellular matrix NYHA, New York Heart Association 6MWT, six minute walk test LVEF, left ventricular ejection fraction LVEDD, left ventricular end-diastolic diameter LVESD, left ventricular end-systolic diameter LVEDV, left ventricular end-diastolic volume LVESV, left ventricular end-systolic volume NT-proBNP, amino-terminal pro-brain natriuretic peptide DCM, dilated cardiomyopathy

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Nitric oxide, lipid peroxidation products, and antioxidants in primary fibromyalgia and correlation with disease severity

institutional ethical committee: Ref. Code: XL VI ECM/A-P9. Conflict of interest Conflict of interest statement: The authors stated that they have no conflicts of interest regarding the publication of this article. References 1 Wolfe F, Hauser W. Fibromyalgia diagnosis and diagnostic criteria. Ann Med 2011; 43: 495–502. 10.3109/07853890.2011.595734 21770697 Wolfe F Hauser W Fibromyalgia diagnosis and diagnostic criteria Ann Med 2011 43 495 502 2 Harden RN, Revivo G, Song S, Nampiaparampil D, Golden G, Kirincic M, et al. A critical

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