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References 1. Annelli J.F., Morrison R.B., Goyal S.M., Bergeland M.E., Mackey W.J., Thawley D.G.: Pig herds having a single reactor to serum antibody tests to Aujeszky’s disease virus. Vet Rec 1991, 128, 49-53. 2. Brown T.M., Osorio F.A., Rock D.L.: Detection of latent pseudorabies virus in swine using in situ hybridization. Vet Microbiol 1990, 24, 273-280. 3. Commission Decision 2008/185/EC of 21 February 2008 on additional guarantees in intra-Community trade of pigs relating to Aujeszky’s disease and criteria to provide information on this disease. OJ L 59, 4

( Vulpes vulpes ): phylogenetic analysis unravels an unexpected epidemiological link. J Wildl Dis 2014, 50, 707–710. 5. Cay A.B., Letellier C.: Isolation of Aujeszky’s disease virus from two hunting dogs in Belgium after hunting wild boars. Vlaams Diergeneeskundig Tijdschrift 2009, 78, 194–195. 6. Commission Decision 2008/185/EC of 21 February on additional guarantees in intra-Community trade of pigs relating to Aujeszky’s disease and criteria to provide information on this disease. OJ L 59, 4.3.2008, 19. 7. Cramer S.D., Campbell G.A., Njaa B.L., Morgan S.E., Smith II S

Dis 2004, 40, 307–310. 8. Cromwijk W.A.: Serological studies in wild boars in the Veluwe area. Tijdschr Diergeneeskd 1995, 120, 364–365. 9. Davison A.J.: Herpesvirus systematics. Vet Microbiol 2010, 143, 52–69. 10. Denzin N., Borgwardt J., Freuling C., Müller T.: Spatio-temporal analysis of the progression of Aujeszky’s disease virus infection in wild boar of Saxony-Anhalt, Germany. Geospatial Health 2013, 8, 203–213. 11. Elbers A.R., Dekkers L.J., van der Giessen J.W.: Sero-surveillance of wild boar in the Netherlands, 1996–1999. Rev Sci Tech 2000, 19, 848–854. 12

Methods 2014, 201:13-19. 6. Grau-Rama L, Segales J: Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine infl uenza virus and Aujeszky’s disease virus in cases of porcine proliferative and necrotizing pneumonia (PNP) in Spain. Vet Microbiol 2007, 119:144-151. 7. Liu JK, Wei CH, Zang XY, Dai AL, Li XH: Multiplex PCR for the simultaneous detection of porcine reproductive and respiratory syndrome virus, classical swine fever virus, and porcine circovirus in pigs. Mol Cell Probes 2013, 27:149-152. 8. Toplak I, Lazić S, Lupulović

. Molecular and Cellular Probes 2013, 27:149-152. 22. Capua RF, Banks M, Tamba M, Calzetta G: Isolation and characterisation of an Aujeszky’s disease virus naturally infecting a wild boar (Sus scrofa). Veterinary Microbiology 1997, 55:141-146. 23. Nišavić J, Milić N: Examination of the activity of glycoprotein HN and F antigens of outer envelope of the parainfluenza virus type 3 by using fusional, hemolytic and hemagglutinating test, in vitro. Acta Veterinaria 2006, 56:431-436. 24. Ros Bascunana C, Bjijrnerot L, Ballagi-Pordhy A, Robertsson JA, Belak S: Detection of

., Patzelt T., Schagemann G., Liess B.: Antibody prevalence of hog cholera, bovine viral diarrhoea and Aujeszky’s disease virus in wild boars in Northern Germany. Dtsch Tierärztl Wochenschr 1993, 100, 330-333. 4. Deng Y., Shan T.L., Tong W., Zheng X.C., Guo Y.Y., Zheng H., Cao S.J., Wen X.T., Tong G.Z.: Genomic characterization of a bovine viral diarrhea virus 1 isolate from swine. Arch Virol 2014, doi 10.1007/s00705-014-2064-9. 5. Deng Y., Sun C.Q., Cao S.J., Lin T., Yuan S.S., Zhang H.B., Zhai S.L., Huang L., Shan T.L., Zheng H., Wen X.T., Tong G.Z.: High prevalence of