Marcin Gołyński, Piotr Dębiak, Magdalena Gołyńska, Ewa Myśliwiec, Piotr Szkodziak, Grzegorz Kalisz, Anna Śmiech, Krzysztof Lutnicki and Marcin Szczepanik
), USA) and the Mann–Whitney U test. The differences between mean values were considered as statistically significant at P ≤ 0.05. Correlations were calculated with the Spearman rank method.
The results of elastography of testes, spermatogenesis, seminiferous tubules diameter, and measurement of body weight and testicular weight are presented in Table 1 .
Results of elastographic findings, spermatogenesis, seminiferous tubules diameter, body weight, and testicular weight in groups E and C
vaccine strains from domesticated poultry to wild birds. Vaccine 2017, 35, 3523–3527. 10.1016/j.vaccine.2017.05.033 Rohaim M.A. El Naggar R.F. Helal A.M. Hussein H.A. Munir M. Reverse spillover of avian viral vaccine strains from domesticated poultry to wild birds Vaccine 2017 35 3523 3527
37 Stephensen C.B., Casebolt D.B., Gangopadhyay N.N.: Phylogenetic analysis of a highly conserved region of the polymerase gene from 11 coronaviruses and development of a consensus polymerase chain reaction assay. Virus Res 1999, 60, 181–189. 10.1016/S
M. Šuleková, M. Smrčová, A. Hudák, M. Heželová and M. Fedorová
: Determination of Carmoisine, Allura red and Ponceau 4R in sweets and soft drinks by differential pulse polarography. J. Food Compos. Anal. , 18, 503—515.
9. Chen, Q. Ch., Mou, S. F., Hou, X. P., Riviello, J. M., Ni, Z. M., 1998: Determination of eight synthetic food dyes in drinks by high-performance ion chromatography. J. Chromatogr. , A, 827, 73—81.
10. Choi, C. K., Dong, M. W., 2005: Chapter 5 — Sample preparation for HPLC analysis of drug products. In Ahuja, S., Dong, M. W.: Handbook of Pharmaceutical Analysis by HPLC . Elsevier, United Kingdom, 123
Barbara Woźniak, Sebastian Witek, Iwona Matraszek-Żuchowska, Alicja Kłopot and Andrzej Posyniak
OJ L 070, 16.03.88, p. 16-18.
4. Council Directive 96/23/EC. 1996 OJ L 125, 23.05.1996, p. 10-32.
5. CRL Guidance Paper, CRLs view on state of the art analytical methods for national residue control plans. December 2007.
6. Doyle E.: Human safety of hormone implants used to promote growth in cattle. A review of the scientific literature. Food Research Institute, University of Wisconsin, July 2000, http://fri.wisc.edu/docs/pdf/hormone.pdf.
7. EURL Reflection paper: natural growth promoting substances in
D. Babangida, A. A. Ibrahim, F. O. Oladayo, A. A. Magaji, B. R. Alkali and A. H. Jibril
1. Abegunde, A. A., Ezeokoli, C. D., Umoh, J. U., Addo, P. B., 1988: Epidemiology of foot and mouth disease in Nigerian cattle viral diseases of animals in Africa. OAU/STRC Scientific publication Lagos Nigeria , 203—212.
2. De-Lahunta, A., Harbel, R. E., 1986: Applied Veterinary Anatomy. W. B. Sounders Company, 4—16.
3. Domingo, E., Baranowski, E., Escarmís, C., Sobrino, F., 2002: Foot-and-mouth disease virus. Comp. Immunol. Microbiol. Infect. Dis. , 25, 297—308.
4. FAO 1997: Prevention and Control of Transboundary
A. Marcin, M. Levkut, V. Revajová, B. Šoltysová and P. Naď
., Rozkot, M., 2005: Influence of lecithin emulsifier on the utilisation of nutrients and growth of piglets after weaning. Czech. J. Anim. Sci., 50, 459-465.
9. Durling, N. E., Catchpole, O. J., Grey, J. B., Webby, R. F., Mitchell, K. A., Yeap Foo, L., Perry, N. B., 2007: Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol-water mixtures. Food Chemistry, 101, 1417-1424.
10. Emmert, J., Sartor, G., Sporer, F., Gummersbach, J., 2004: Determination of α-/β-thujone and related terpemens in absinthe using
Anna Madejska, Mirosław Michalski, Marzena Pawul-Gruba and Jacek Osek
18 column of 150 × 4.6 mm, particle size 3 μm, connected to a C18 precolumn of 10 × 3 mm (Agela Technologies, USA). The mobile phase, consisting of 15% methanol in 0.1 M potassium dihydrogen phosphate (150/850, v/v) with 1.6 mM 1-octanesulphonic acid, was applied under isocratic conditions. The column oven temperature was maintained at 25°C, the flow rate at 0.5 mL/min, and the injection volume was 20 μL. The UV detection was monitored at 215 nm.
The range of the method for hard cheeses was 4.25–420 mg/kg and the limits of detection and quantification were 3
each dNTP, 10 pmol of each primer, 1 U of Taq polymerase, and 2 μL (16–50 ng/mL) of DNA were mixed for each 25 μL of PCR reaction. PCR amplification was performed with an initial denaturation step of 5 min at 94°C, followed by 35 cycles of 3 s at 94°C, 4 s at 50°C, 35 s at 72°C, and a final extension step of 10 min at 72°C. Next, a 1.5% agarose gel was prepared and the PCR products were run on the gel at 90 volts for 1 h. Subsequently, imaging was performed using a UV transilluminator (EC3 ChemiHR 410 Imaging System, UVP, USA).
.I., Son R., Maimunah M., Lee H.Y., Wong W.C., Elexson N.: MPN-PCR detection and antimicrobial resistance of Listeria monocytogenes isolated from raw and ready-to-eat foods in Malaysia. Food Control 2012, 28, 309–314. 10.1016/j.foodcont.2012.05.030 Marian M.N. Sharifah Aminah S.M. Zuraini M.I. Son R. Maimunah M. Lee H.Y. Wong W.C. Elexson N. MPN-PCR detection and antimicrobial resistance of Listeria monocytogenes isolated from raw and ready-to-eat foods in Malaysia Food Control 2012 28 309 314
30 Meldrum R.J., Ellis P.W., Mannion P.T., Halstead D., Garsiede J
Turhan Turan, Hakan Işıdan, Mustafa Ozan Atasoy and Bünyamin Irehan
Extraction Kit (Vivantis Technologies, Malaysia) according to the manufacturer’s instructions. Eluted nucleic acids were stored at −80ºC until use.
Reverse transcription polymerase chain reaction (RT-PCR) The cDNA synthesis was carried out in a 25 μL final volume containing 4 μL of RNA extract, 10 mM of deoxynucleoside triphosphate (dNTP), 2.5 μL of 10× RT buffer (50 mM Tris-HCl (pH 8.3 at 25°C), 75 mM of KCl, 3 mM of MgCl 2 , and 10 mM of DTT), 50 ng of the random hexamer, 40 U of RNasin, and 200 U of M-MuLV Reverse Transcriptase RNase H - (Vivantis, Germany). The