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Amitraz marker residues in honey from honeybee colonies treated with Apiwarol

acetamiprid-D3 as an internal standard. Next, 10 mL of water and glass beads were added, and the sample was shaken. The sample was extracted with 10 mL of 1% acetic acid in acetonitrile and shaken again. Sodium acetate in a 1 g mass and magnesium sulphate at 4 g were added, the sample was shaken a further time and centrifuged. Then, a 7 mL aliquot of the acetonitrile phase was subjected to clean-up by dispersive solid phase extraction (D-SPE), using 350 mg of PSA, 350 mg of Z-Sep +, and 1,050 mg of magnesium sulphate. After shaking and centrifugation, 0.5 mL of supernatant

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Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing

-gel method. First, certain quantities of phosphoric pentoxide (P 2 O 5 ) and calcium nitrate tetrohydrate Ca(NO 3 ) 2 .4H 2 O were dissolved in absolute ethyl alcohol with a pH of 10.5 to form 0.5 mol/L and 1.67 mol/L solutions, respectively. After this, these solutions were mixed together to obtain Ca/P molar ratios of 1.5 (TCP) and 1.67 (HA). The resultant mixture was stirred for 30 min and then was heated in a water bath at 60°C for 1 h. The obtained gel was subsequently dried at 80°C for 24 h in an air oven and calcined at 700°C for 3 h in a furnace. After that, the

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Plasma concentration of norepinephrine, β-endorphin, and substance P in lame dairy cows

groups had mean values of 0.25 ± 0.09 ng/mL, 0.32 ± 0.13 ng/mL, and 0.42 ± 0.12 ng/mL ( Fig. 1c ). In contrast, cows in the MS 3 group showed a significant mean increase of 0.61 ± 0.12 ng/mL compared to MS 0 cows (P = 0.000043) ( Table 2 ). Fig. 1 Mean (+SD) plasma concentration of norepinephrine (a), β-endorphin (b), and substance P (c) of chronically lame and control dairy cows (N = 25 per MS). * – significant differences compared to MS 0, P < 0.05 Table 2 Linear mixed effect model of mobility scoring (MS) on norepinephrine, β-endorphin, and substance

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DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

Zusammensetzung. Agrarforschung 2003, 10, I-VIII. 17. Fumiere O., Dubois M., Baeten V., von Holst C., Berben G.: Effective PCR detection of animal species in highly processed animal byproducts and compound feeds. Anal Bioanal Chem 2006, 385, 1045-1054. 18. Fumiere O., Veys P., Boix A., von Holst C., Baeten V., Berben G.: Methods of detection, species identification and quantification of processed animal proteins in feedingstuffs. Biotechnol Agron Soc Environ 2009, 13, 59-70. 19. Gizzi G., von Holst Ch.: Determination of processed

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Effects of newly developed synbiotic and commercial probiotic products on the haematological indices, serum cytokines, acute phase proteins concentration, and serum immunoglobulins amount in sows and growing pigs – a pilot study

Lactobacillus plantarum ŁOCK 0860 Lactobacillus pentosus ŁOCK 1094 B B Saccharomyces cerevisiae ŁOCK 0118 Lactobacillus rhamnosus ŁOCK 1087 Inulin Lactobacillus reuteri ŁOCK 1092 Lactobacillus plantarum ŁOCK 0860 0.5 kg/ton of basal diet 10 days before farrowing until from 2 weeks of age until slaughter Lactobacillus pentosus ŁOCK 1094 weaning C C Saccharomyces cerevisiae ŁOCK 0118 Lactobacillus rhamnosus ŁOCK 1087

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Microbiological quality of farmed grass carp, bighead carp, Siberian sturgeon, and wels catfish from Eastern Poland

-positive staphylococci and E. coli (below 1 log cfu/g) were found in samples of the examined fish species. Fig. 1 Microbial analysis of aerobic counts (A), psychrophilic counts (B), Enterobacteriaceae counts (C), and Staphylococcus counts (D) (mean ±SD, log cfu/ g) No statistically significant correlations (P > 0.05) between the levels of particular bacteria were found. Discussion The aerobic plate count (APC) microbiological limits recommended by the ICMSF for fresh fish are specified as the threshold value for the number of bacteria m = 5 × 10 5 ( i.e log 5

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Screening for circulating miR-208a and -b in different cardiac arrhythmias of dogs

-208b, cel-miR-39-5p, and miR-16), 0.3 μL of 100 mM dNTPs, 3 μL of multiscribe reverse transcriptase (50U/μL), 1.5 of 10 × RT buffer, 0.19 μL of RNase inhibitor (20U/μL), 2.01 μL of nuclease-free water, and 5 μL of the RNA sample. The complete reaction mix was incubated on ice for 5 min and then underwent thermocycling (16°C for 30 min, 42°C for 30 min, 85°C for 5 min, and 4°C for ∞). The cDNA was stored at −20°C until use. Quantitative PCR was performed to approximate the miR-208, miR-208b, cel-miR-39-5p, and miR-16 concentrations in the samples before the droplet

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Characteristics, immunological events, and diagnostics of Babesia spp. infection, with emphasis on Babesia canis

L., Baneth G., Gorenflot A., Schetters T.P.: Classification of Babesia canis strains in Europe based on polymorphism of the Bc28.1-gene from the Babesia canis Bc28 multigene family. Vet Parasitol 2015, doi:10.1016/j.vetpar.2015.05.028 11. Carret C., Walas F., Carcy B., Grande N., Précigout E., Moubri K., Schetters T.P., Gorenflot A.: Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes. J Eukaryot

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Effects of packaging methods on shelf life of ratite meats

proteins. J Agr Food Chem 2008, 56, 1488-1494. 44. Sakowska A., Guzek D., Sun D.W. Wierzbicka A.: Effects of 0.5% carbon monoxide in modified atmosphere packaging on selected quality attributes of M. Longissimus dorsi beef steaks. J Food Process Eng 2016, doi: 10.1111/jfpe.12517. 45. Seydim A.C., Acton J.C., Hall M.A., Dawson P.L.: Effects of packaging atmospheres on shelf-life quality of ground ostrich meat. Meat Sci 2006, 73, 503-510. 46. Vázquez B.I., Carriera L., Franco C., Fente C., Cepeda A., Barros- Velázquez J

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Use of a new LC-MS method for the determination of pyrrolizidine alkaloids in feeds

seven parameters which were slightly changed. The effects of change of percentage of sulphuric acid (0.05 M, 0.1 M), volume of elution mixture (12 mL, 10 mL), evaporation temperature (40ºC, 45ºC), percentage of formic acid in the mobile phase (0.2%, 0.18%), injection volume (5 μL, 4.5 μL), thermostat temperature (30°C, 27°C), and flow rate (0.6 mL min -1 , 0.63 mL min -1 ) were evaluated. Student’s t- test was used to determine the impact of changes in individual parameters on the results of the analysis. A stability test was performed on SPE purified extracts

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