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Myelodysplastic syndrome with myelofibrosis in a 12-year-old patient – A case report

Abstract

Myelodysplastic syndromes are a heterogeneous group of clonal disorders characterized by peripheral blood cytopenia and normal or hypercellular bone marrow with dysplasia in more than one blood cell lineage, unfavorable prognosis, and lack of response to treatment. We present the case of a 12-year-old male patient who was referred to the Hematology and Oncology Department of Pediatric Clinic I Târgu- Mures in May 2016, with splenomegaly and pancytopenia. The osteomedullary biopsy revealed myelofibrosis, discrete dysplasia of the myeloid series and megakaryocytes, blasts CD34+ approximately 10%, which led to the diagnosis of myelodysplastic syndrome with myelofibrosis. The myeloid precursors indicated a high risk of transformation into acute myeloid leukemia, so chemotherapy associated with corticosteroids was started, leading to slight improvements. Although myelodysplastic syndrome associated with myelofibrosis is rare at this age, despite the treatment and favorable progression in the case presented, in the absence of hematopoietic stem cell transplantation the prognosis remains unfavorable.

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Implantable orthotopic bladder cancer model in Wistar rats: A pilot and feasibility study

Abstract

Purpose: The implantable bladder cancer (BC) models allow the researchers to perform rapid and useful experiments for BC. We investigated the implantation success of BC cells obtained from Wistar rats (grown in vitro), into bladders of syngeneic Wistar rats, which are commonly used in the laboratories.

Methods: The Nara Bladder Tumor No.2 (NBT-II) BC cells induced with 4-hydroxybutylnitrosamine were grown with passages in Kocaeli University Center for Stem-Cell and Gene-Therapies. After urothelial denudation, 2x106 NBT-II cells were then implanted into bladders of 24 female Wistar rats (aged 7-8 weeks). The rats were randomly divided into four experimental groups; three instillation groups (8 per group) and one sham-operated control group consisting of 6 rats. First, second and third instillation groups were sacrificed at days 7, 14, and 21, respectively, and, bladders were histopathologically evaluated for BC according to WHO / International Society of Urological Pathology.

Results: All tumors were pT1 (including 1 rat that prematurely died at 5th day), except one rat that died prematurely at 8th day had pT2 tumor. Implantation rates were 28.58% (2/7) in the first group, and 42.85% (3/7) in the second, for a cumulative rate of 35.71% (5/14) in these two-groups (until 14th day). Interestingly, there was no tumor in the third group, but there was an inflammatory granulation tissue.

Conclusion: Seeding NBT-II cells into bladders of Wistar rats was described, successfully tested and demonstrated in this study. This implantable BC model of Wistar rats may be improved to increase the success rate of BC cell implantation in new studies with higher number of animals.

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CX3CR1 expression and megakaryocytic series assessment on bone marrow biopsies in acquired aplastic anemia. Correlations with hematological parameters.

quiescence and promote post-injury regeneration of hematopoietic stem cells. Nat Med. 2014;20(11):1321-6. DOI: 10.1038/nm.3706 11. Bruns I, Lucas D, Pinho S, Ahmed J, Lambert MP, Kunisaki Y, et al. Megakaryocytes regulate hematopoietic stem cell quiescence through CXCL4 secretion. Nat Med. 2014;20(11):1315-20. DOI: 10.1038/nm.3707 12. Nakamura-Ishizu A, Takubo K, Fujioka M, Suda T. Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin. Biochem Biophys Res Commun. 2014;454(2):353-7. DOI: 10.1016/j.bbrc.2014.10.095 13

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Cynomorium Songaricum may protect against spermatogenic damage caused by cyclophosphamide in SD rats

, Cattoretti G, Manova K, Sukhwani M, et al. Essential role of Plzf in maintenance of spermatogonial stem cells. Nat Genet. 2004;36(6):653-9. DOI: 10.1038/ng1367 17. Takashima S, Kanatsu-Shinohara M, Tanaka T, Morimoto H, Inoue K, Ogonuki N, et al. Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal. Stem cell reports. 2015;4(3):489-502. DOI: 10.1016/j.stem-cr.2015.01.010 18. Morimoto H, Kanatsu-Shinohara M, Takashima S, Chuma S, Nakatsuji N, Takehashi M, et al. Phenotypic plasticity of mouse spermatogonial

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Effects of miR-99a on the migration and proliferation of glioma cells

.3171/2013.1.JNS121 13. Chen YS, Chen ZP. Vasculogenic mimicry: a novel target for glioma therapy. Chin J Cancer. 2014;33(2):74-9. DOI: 10.5732/cjc.012.10292 14. Wang XJ, Xiang BY, Ding YH, Chen L, Zou H, Mou XZ, et al. Human menstrual blood-derived mesenchymal stem cells as a cellular vehicle for malignant glioma gene therapy. Oncotarget. 2017;8(35):58309-21. DOI: 10.18632/oncotarget.17621 15. Portnow J, Synold TW, Badie B, Tirughana R, Lacey SF, D’Apuzzo M, et al. Neural Stem Cell-Based Anticancer Gene Therapy: A First

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The Value of FLT3, NPM1 and DNMT3A Gene Mutation Analysis in Acute Myeloid Leukemia Diagnosis

leukemia: case report and literature review. Rev Romana Med Lab. 2017;25(2):165-79. DOI: 10.1515/rrlm-2017-0013 6. Kumar D, Mehta A, Panigrahi MK, Nath S, Saikia KK. DNMT3A (R882) mutation features and prognostic effect in acute myeloid leukemia in Coexistent with NPM1 and FLT3 mutations. Hematol Oncol Stem Cell Ther. 2018 Jun;11(2):82-9. DOI: 10.1016/j.hemonc.2017.09.004 7. Tang S, Shen H, Mao X, Dai H, Zhu X, Xue S, et al. FLT3-ITD with DNMT3A R882 double mutation is a poor prognostic factor in Chinese patients with acute myeloid leukemia after chemotherapy

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The impact of immunological and biomolecular investigations on the outcome of children with acute lymphoblastic leukemia - experience of IIIrd Paediatric Clinic Timisoara

; 37(4):223-9. DOI: 10.1016/j.bjhh.2015.03.009 20. Pui C-H, Campana D, Pei D, Bowman WP, Sandlund JT, Kaste SC, et al. Treating childhood acute lymphoblastic leukemia without cranial irradiation. N Engl J Med. 2009 Jun 25; 360(26):2730-41. DOI: 10.1056/NEJMoa0900386 21. Gökbuget N, Kneba M, Raff T, Trautmann H, Bartram C-R, Arnold R, et al. Adult patients with acute lymphoblastic leukemia and molecular failure display a poor prognosis and are candidates for stem cell transplantation and targeted therapies. Blood. 2012 Aug 30

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Association of Human Leukocyte Antigen and Cytomegalovirus disease after Kidney Transplantation

, Naumova E. HLA-A11 Increases the Risk for Post-Transplant Active Cytomegalovirus Infection in Kidney Graft Recipients. BANTAO Journal. 2010;8(1):44-9. 16. Kekik C, Besisik BK, Seyhun Y, Oguz FS, Sargin D, Carin MN. Relationship Between HLA Tissue Type, CMV Infection, and Acute Graft-vs-Host Disease After Allogeneic Hematopoietic Stem Cell Transplantation: Single-Center Experience. Transplant Proc. 2009; 41(9):3515-970. DOI: 10.1016/j.transproceed. 2009.04.017 17. Bal Z, Uyar ME, Tutal E, Erdogan E, Colak T, Sezer S, et al

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N-3 polyunsaturated fatty acids induce granulopoiesis and early monocyte polarization in the bone marrow of a tMCAO rat model

stem cell niche: how hematopoietic stem cells generate inflammatory monocytes after stroke. Circ Res. 2015 Jan;116(3):389-92. DOI: 10.1161/CIRCRESAHA.114.305678 18. Orădan A, Huțanu A, Horváth E, Chiriac L, Dobreanu M. Improved rat stroke model by intraluminal middle cerebral artery occlusion: a special emphasis on surgical technique. Health Problems of Civilization 2017;11:202-10. DOI: 10.5114/hpc.2017.70008 19. Mezei T, Horváth E, Turcu M, Gurzu S, Raica M, Jung I. Microvascular density in non-Hodgkin B-cell lymphomas measured using digital morphometry

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Blastic plasmacytoid dendritic cell neoplasia - a rare type of acute leukemia

allogeneic stem cell transplantation with moderately reduced conditioning intensity. Biol Blood Marrow Transplant. 2011;17(8):1250-4. DOI: 10.1016/j.bbmt.2010.12.706 17. Garnache-Ottou F, Chaperot L, Biichle S, Ferrand C, Remy-Martin JP, Deconinck E, et al. Expression of the myeloid-associated marker CD33 is not an exclusive factor for leukemic plasmacytoid dendritic cells. Blood. 2005;105(3):1256-64. DOI: 10.1182/ blood-2004-06-2416 18. Bueno C, Almeida J, Lucio P, Marco J, Garcia R, de Pablos JM, et al. Incidence and characteristics of CD4

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