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Xylazine regulates the release of glycine and aspartic acid in rat brain

/sugar mixture for 10 min and placed in 1 mL of 0.1% (v/v) Triton X-100 for 10 min at room temperature. After two washes with phosphate-buffered saline (PBS), the cells were incubated in 10% foetal bovine serum (Invitrogen, USA) for 1 h. Then the cells were incubated overnight at 4°C with a mouse anti-MAP2 antibody (Invitrogen, USA), followed by goat anti-mouse IgG (1:100; Santa Cruz Biotechnology, USA) for 1 h and washed three times with PBS. Treatment management Neurons harvested from foetal rats were treated with one of the following xylazine concentrations: 15 μg

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Ecological Factors of Transmission, Persistence and Circulation of Pathogens In Bat Populations

REFERENCES 1. Adesiyun, A. A., Stewart-Johnson, A., Thompson, N. N., 2009: Isolation of enteric pathogens from bats in Trinidad. J. Wildl. Dis. , 45 (4), 952—961. DOI: 10.7589/0090-3558-45.4.952. 2. Allocati, N., Petrucci, A. G., Di Giovanni, P., Masulli, M., Di Illio, C., De Laurenzi, V., 2016: Bat-man disease transmission: zoonotic pathogens from wildlife reservoirs to human populations. In Cell Death Discovery 2 , Article No. 16048. . 3. Bartlett, M., 1957: Measles periodicity and

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Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs

pig was pre-medicated subcutaneously with 0.05 mg/kg of atropine (Beijing Shuanghe Pharmaceutical, China) and 15 min later intramuscular injections of xylazine (1 mg/kg) and ketamine hydrochloride (10 mg/kg) were given and intravenous administration of propofol (3.5 mg/kg) was used to induce anaesthesia. The animal was intubated with a 6.0–7.0 mm tracheal tube and maintained with 1.5%–3% isoflurane in oxygen after intubation. Laparoscopic hepatic ischaemia-reperfusion and partial resection were performed after establishing a 4-portal approach. After laparoscopic

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In vitro cultivation and immunostaining of Lawsonia intracellularis strains

. Vet Microbiol 2003, 91, 135-145. 6. Huerta B., Arenas A., Carrasco L., Maldonado A., Tarradas C., Carbonero A., Perea A.: Comparison of diagnostic techniques for porcine proliferative enteropathy ( Lawsonia intracellularis infection). J Comp Pathol 2003, 129, 179-185. 7. Hwang J.M., Lee J.H., Yeh J.Y.: A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures. BMC Res Notes 2012, 5, 78, doi: 10.1186/1756-0500-5-78. 8. Jacobson M., Fellström C., Jensen-Warn M.: Porcine

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Clostridium perfringens spores in Polish honey samples

agar (FAA) medium plates. The first of these media comprised peptic digest of animal tissue 10 g/L, meat extract 10 g/L, sodium chloride 5 g/L, lactose 12 g/L, neutral red 0.032 g/L, and agar 10 g/L, with final pH 7.0 ± 0.2 at 25°C and the second peptone 23 g/L, sodium chloride 5 g/L, soluble starch 1 g/L, sodium bicarbonate 0.4 g/L, glucose 1 g/L, sodium pyruvate 1 g/L, L-cysteine HCl×H 2 O 0.5 g/L, sodium pyrophosphate 0.25 g/L, L-arginine 1 g/L, sodium succinate 0.5 g/L, Hemin 0.01 g/L, vitamin K 0.001 g/L, and agar 12 g/L, with final pH 7.2 ±0.2 at 25°C. The

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GLP-1 localisation and proglucagon gene expression in healthy and diabetic mouse ileum

measured, and mRNA was isolated from the total RNA by using oligo (dT) primers. The obtained mRNA was reverse transcribed to complementary DNA (cDNA) by using dNTPs, Moloney murine leukaemia virus reverse transcriptase enzyme, buffer, RNasin, and nuclease-free water. To obtain cDNAs, the prepared master mix was added to tubes containing the mRNA and the tubes were placed in a thermocycler at 37°C for 1 h, 95°C for 5 min, and 4°C for 10 min. To amplify the target gene in the thermocycler, the obtained cDNAs were added to gene-specific primers ( Table 1 ) and a mixture

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Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves

dimer (KLP-602, Lydium-KLP) on cellular and humoral defence mechanisms and cytokine levels in piglets). Materiały II Krajowego Sympozjum Immunologów Wet. (Proceedings of the II National Symposium on Veterinary Immunology) Świnoujście 1997 175 23 Soehnlen M.K., Aydin A., Lengerich E.J., Houser B.A., Fenton G.D., Lysczek H.R., Burns C.M., Byler L.I., Hattel A.L., Wolfgang D.R., Jayarao B.M.: Blinded, controlled field trial of two commercially available Mycoplasma bovis bacterin vaccines in veal calves. Vaccine 2011, 29, 5347–5354. 10.1016/j.vaccine.2011.05

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Experimental infection with T. canis and T. leonina in farm mink (Neovison vison)

. Dubrovskis V., Plume I., Straume I.: Investigation of biogas production from mink and cow manure. Engineering Rural Develop, Jeglava 28-29.05.2009, doi: 5. Fillaux J., Magnaval J.F.: Laboratory diagnosis of human toxocariasis. Vet Parasitol 2013, 193, 327–336. 6. Foster G.W., Cunningham M.W., Kinsella J.M., Owen M.: Parasitic helminths of free-ranging mink ( Neovison vison ) from Southern Florida. J Parasitol 2007, 93, 945–946. 7. Gawor J., Borecka A., Marczyńska M., Dobosz S., Żarnowska-Prymek H.: Risk of human toxocarosis in Poland due to

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Lack of association between Epstein–Barr virus and mammary tumours in dogs

: recent progress and applications. Vet Immunol Immunopathol 2014, 159, 192–201. 10.1016/j.vetimm.2014.02.016 Ito D. Frantz A.M. Modiano J.F. Canine lymphoma as a comparative model for human non-Hodgkin lymphoma: recent progress and applications Vet Immunol Immunopathol 2014 159 192 – 201 16 Karayannopoulou M., Kaldrymidou E., Constantinidis T.C., Dessiris A.: Histological grading and prognosis in dogs with mammary carcinomas: application of a human grading method. J Comp Pathol 2005, 133, 246–252. 10.1016/j.jcpa.2005.05.003 Karayannopoulou M

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Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat

application of the developed method for routine practice. Material and Methods Sample preparation Samples were prepared from meat of pigs ( Sus scrofa ferus domestica ), cattle ( Bos taurus ), and poultry ( Gallus gallus domesticus ). Meat samples of a single species had muscle fat and fascia removed and were then ground. Meat mixtures of different animal species were made by blending 50%, 25%, 10%, 5%, 4%, 3%, 2%, 0.5%, or 0.2% meat from other species. Laboratory sample precursors of 50 g were weighed out of standardised samples of meat and meat mixtures, and

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