Katarzyna Wojcicka-Lorenowicz, Krzysztof Kostro, Urszula Lisiecka and Bolesław Gąsiorek
using the commercial Phagotest kit (Orpegen Pharma, Germany), according to the manufacturer’s instructions. Heparinised peripheral blood (100 μL) was incubated with 20 μL of FITC-labelled heat-killed E . coli (10 cfu) for 20 min at 37°C, a negative control remained in the ice bath for 20 min. At the end of the incubation, all the samples were placed in the ice bath in order to stop phagocytosis. A volume of 100 μL of ice-cold Quenching Solution was added to each sample. The samples were mixed (by vortex mixer) for 2 min at 25°C. Then, 3 mL of cold washing solution
1. Al-Sabi, M. N. S., Kapel, Ch. M. O., Johansson, A., Espersen, M. C., Koch, J., Willesen, J. L., 2013: A coprological investigation of gastrointestinal and cardiopulmonary parasites in hunting dogs in Denmark. Vet. Parasitol. , 196 (3—4), 366—372.
2. Arneberg, P., Skorping, A., Grenfell, B., Read, A. F., 1998: Host densities as determinants of abundance in parasite communities. Proceedings of the Royal Society of London B: Biological Sciences , 265 (1043), 1283˙1289. DOI: 10.1098/rspb.1998—0431.
3. Batchelor, D. J., Tzannes, S
. Fatehullah A. Jagan I. Nagaraju M. Bingham V. Campbell F.C. PTEN regulates colorectal epithelial apoptosis through Cdc42 signalling Br J Cancer 2011 105 1313 1321
3 Dorneles E.M.S., Teixeira-Carvalho A., Araujo M.S.S., Sriranganathan N., Lage A.P.: Immune response triggered by Brucella abortus following infection or vaccination. Vaccine 2015, 33, 3659–3666. 10.1016/j.vaccine.2015.05.057 Dorneles E.M.S. Teixeira-Carvalho A. Araujo M.S.S. Sriranganathan N. Lage A.P. Immune response triggered by Brucella abortus following infection or vaccination Vaccine 2015 33 3659
to 4 days after the exposure to different doses of Nerium oleander extract in four experimental groups (Group 1, Group 2, Group 3, and Group 4 which received 10, 12.5, 15, and 20 mg/kg b.w. of the prepared extract of the plant respectively) and the control (group 5) which received only normal saline
177.5 ± 34.7 a Different letters in the same column show significant differences between mice and rats (P < 0.05)
171.7 ± 36.7 c
175.8 ± 31.4 e
Marcin Weiner, Wioletta Żukiewicz-Sobczak, Małgorzata Tokarska-Rodak, Dorota Plewik, Anna Pańczuk, Marta Siłuch, Jerzy Zagórski, Paweł Sobczak, Tomasz Chmielewski, Stanisława Tylewska-Wierzbanowska, Mariia Shkilna, Mykhailo Korda, Ivan Klisch, Mykhailo Andreychyn and Mariana Pavliuk
Moro C., Vaumourin E., Michelet L., Tran F.H., Devillers E., Cosson J.F., Gasqui P., Van V.T., Mavingui P.: Coinfection of ticks: the rule rather than the exception. PLoS Negl Trop Dis 2016, 10 (http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004539 2018. 05.30. Moutailler S. Valiente Moro C. Vaumourin E. Michelet L. Tran F.H. Devillers E. Cosson J.F. Gasqui P. Van V.T. Mavingui P. Coinfection of ticks: the rule rather than the exception PLoS Negl Trop Dis 2016 10 (http
Sylwia Tobólska, Sylwia Terpiłowska, Jerzy Jaroszewski and Andrzej Krzysztof Siwicki
of lyophilised Salmonella typhimurium mutant strains (TA97a, TA98, TA100, TA102, and TA1535) and were incubated in the bottle overnight at 37 ° C.
The final concentrations of inosine pranobex (0.1, 0.5, 1, 5, 10, 50, 100, 500, and 1,000 μg/mL) were obtained by dilution in sterile distilled water. The samples to be tested were sterilised using a 0.22 μm membrane filter. The reaction mixture was prepared according to the manufacturer’s instructions as follows: 43.24 mL (A) + 9.5 mL (B) + 4.76 mL (C) + 2.38 mL (D) + 0.12 mL (E) where A is Davis-Mingioli salts
Tomasz Śniegocki, Marta Giergiel, Bartosz Sell and Andrzej Posyniak
-aminohydantoin (AHD), semicarbazide (SEM), 3-amino-2-oxazolidone (AOZ), and the internal standards 1,2-(15N2, 13C) SEM, AMOZ-d5, (3C13) AHD, and AOZ-d4 were purchased from the Sigma-Aldrich Chemical Company (Germany).
UHPLC-MS/MS The UHPLC-MS/MS system consisted of an AB Sciex ExionLC UHPLC system connected to an AB Sciex API 5500 Qtrap mass spectrometer (AB Sciex, Canada). Analyst 1.6.3 software (AB Sciex) controlled the UHPLC-MS/MS system and Multiquant 3.2 (AB Sciex) was used to process the data. The MS/MS was operated as previously described with some modifications ( 10
Marta Skowron, Jolanta Zalejska-Fiolka, Urszula Błaszczyk, Ewa Chwalińska, Aleksander Owczarek and Ewa Birkner
. Barrier P. Danford D. Ernst N.D. Grundy S.M. Leveille G.A. Van Horn L. Williams C.L. Booth S.L. Dietary fat consumption and health Nutr Rev 1998 56 23 – 28
11 Niemann B., Rohrbach S., Miller M.R., Newby D.E., Fuster V., Kovacic J.C.: Oxidative stress and cardiovascular risk: obesity, diabetes, smoking, and pollution: Part 3 of a 3-Part Series. J Am Coll Cardiol 2017, 70, 230–251. doi: 10.1016/j.jacc.2017.05.043. 10.1016/j.jacc.2017.05.043
Niemann B. Rohrbach S. Miller M.R. Newby D.E. Fuster V. Kovacic J.C. Oxidative stress and
Teresa Kamińska-Gibas, Ilgiz Irnazarow, Joanna Szczygieł and Patrycja Jurecka
to a reaction mix, containing 12.5 μL of GoTaq qPCR Master Mix 2x, 5.5 μL of nuclease-free H 2 O, and 1 μL (concentration 10 μM) of each primer, making a total volume of 25 μL per reaction. Each sample was prepared in duplicate. A non-template control was included for each set of primers. The following cycling profile was applied: 50°C for 2 min, 95°C for 10 min, 40 cycles of 95°C for 15 s, 60°C for 1 min, and 72°C for 30 s, with the final holding stage at 60°C for 1 min. As a control, the 40 S ribosomal protein S11 gene was used. The comparative 2 -ΔΔCt method