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Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation

described previously. In order to examine the cellular viability post encapsulation, sections of 1 mm thickness were taken from the centre of alginate discs containing 3T3 cells using a sterilized blade. The sections were immersed in 0.2 μL calceinacetoxymethylester (calcein-AM) for 15 min and 2.5 μL propidiumiodide (PI) for 5 min in S-DMEM at 37 o C. The calcein-AM was cleaved to form calcein in the presence of esterases in live cells resulting in photon emission at a wavelength around 515 nm. In the case of dead-cells, the PI penetrated the cell and nuclear membrane

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Testing miniaturized electrodes for impedance measurements within the β-dispersion – a practical approach

plasma) surrounded by an insulating membrane (plasma membrane). The extracellular space is filled with electrolyte as well, but may have a very distinct conductivity with respect to the conductivity of the cytoplasm. Although the cytoplasm contains membrane systems (e.g. endoplasmatic reticulum, Golgi apparatus, nuclear membrane or other membrane enclosed organelles) a pure resistive model for this part is often sufficient for a practical approach [ 29 ; 30 ]. Since intact cell membranes show a low conductance, the low frequency current (f < 10 kHz) will

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Structure and Management of the National Automated System for Permanent Control of the Radiation Gamma Background in Bulgaria

References Bulgarian Nuclear Regulatory Agency. (2016). Annual report of 2015, Sofia: Bulgarian nuclear regulatory agency, available at: The Council of Ministers. (2011). National programme on disaster the 2014 -2018, Sofia: the Council of Ministers. The Council of Ministers. (2013). National report on the status and protection of the environment in Bulgaria in 2012 of the EEA, Sofia: The Council of Ministers. The Council of Ministers

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The Search for Classified Information – The Real Fight in the Cold War

-storyarchive/intelligence-human-intelligence.html [10] Nuclear spy ring consists of Julius and Ethel Rosenberg, Harry Gold, Klaus Fuchs, David and Ruth Greenglass [11] Kim Plilby [12] [13] [14] Dangle - A person sent by the intelligence agency of his or her own country who approaches an intelligence agency in the hope of being recruited as a spy so as to allow a double agent operation

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The Components of Non-Punitive Environment in Nursing

.ssci.2014.06.007 Flin, R. (2007). Measuring safety culture in healthcare: A case for accurate diagnosis. Safety Science, 45(6), 653-667. Garg, A. (1999). Long-term effectiveness of “zero-lift program” in seven nursing homes and one hospital, Cincinnati, OH: National Institute for Occupational Safety and Health, Center for Disease Control and Prevention, U.S. Department of Health & Human services; 106. Gershon, R. M., Karkashian C. D., & Grosch, J. W. (2000). Hospital safety climate

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