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European oak decline phenomenon in relation to climatic changes

Bulletin , 24(1), 221–232. Ceisla W.M., Donauber E. 1994. Decline and dieback of forest trees and forests. A global overview. FAO Forestry Papers , 120, 90 pp. Cleary M.R., van der Kamp B.J., Morrison D.J. 2012. Pathogenicity and virulence of Armillaria sinapina and host response to infection in Douglas-fir, western hemlock, and western red cedar in the southern interior of British Columbia. Forest Pathology , 42, 481–491. Cleary M, Holmes T. 2011. Traumatic resin duct formation in the phloem of western red cedar ( Thuja plicata ) following abiotic

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Typing of Listeria Monocytogenes Isolated from Slaughtered Cattle and Beef Meat

Abstract

A total of 135 L. monocytogenes strains isolated from slaughtered cattle and beef meat were tested by the pulsed field gel electrophoresis (PFGE). The AscI restriction analysis revealed a genetic heterogeneity among investigated isolates since 31, 9, and 35 profiles were distinguished among hide, carcass, and meat strains, respectively. The PFGE profiles of the isolates were also analysed in relation to serotypes, virulence genes, and antimicrobial resistance. It was shown that strains displaying the same PFGE type were of the same serotype while correlation between pulsotype and antimicrobial resistance was poor. The obtained results suggest that a cross-contamination between bovine hides and carcasses may occur during the slaughter process. Moreover, identification of identical PFGE types among L. monocytogenes found during a study period may suggest a common source of contamination or presence of persistent strains able to survive for a long time. These results emphasise the importance of molecular subtyping methods, including PFGE, in monitoring and tracking pathogen contamination along food chain.

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Characteristics of Staphylococcus Xylosus Isolated from Subclinical Mastitis in Cows

Abstract

The aim of the present study was to determine virulence factors and antibiotic susceptibility of Staphylococcus xylosus isolated from subclinical mastitis in cows. The material consisted of 42 isolates of S. xylosus obtained from 276 samples of milk collected from cows with subclinical mastitis. The isolates were obtained from the udder secretions of 33 cows from farms in the Lublin region (Poland). S. xylosus was found in 15.2% of tested milk samples. The study did not reveal any macroscopic changes in the milk or symptoms in the cow’s body. The number of somatic cells in milk samples ranged from 245,000 to 416,000/ml of milk (on average 268,000/ml of milk). The ability to produce slime was observed in 42.9% of S. xylosus isolates. None of the tested isolates demonstrated the ability to produce protease or cause haemolysis. Five isolates of S. xylosus (11.9%) were classified to the methicillin-resistant group. The mecA gene was not found in any of these isolates. The enzyme β-lactamase was detected in 28.6% of S. xylosus isolates. The highest efficacy against S. xylosus was demonstrated for cephalosporin antibiotics: cefacetrile and cefoperazone (80.1% and 76.2% of susceptible isolates of S. xylosus, respectively). A significant quantity of isolates was resistant to streptomycin, linkomycin, penicillin and neomycin (approximately 10% of susceptible isolates of S. xylosus).

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Genotypic virulence markers of Yersinia enterocolitica O:9 isolated from pigs and wild boars serologically positive and negative for brucellosis

M., Rastawicki W.: Evaluation of usefulness for selected virulence markers for identifying pathogenic Yersinia enterocolitica strains. IV. Genes myfA and myfC and ureC. Med Dosw Mikrobiol 2002, 54, 347-355. 4. Gyuranecz M., Erdelyi K., Makrai L., Fodor L., Szepe B., Raczne Meszaros A., Dan A., Dencso L., Fassang E., Szeredi L.: Brucellosis of the European brown hare (Lepus europaeus). J Comp Path 2011, 145, 1-5. 5. Harnett N., Lin Y.P., Krishahn C.: Detection of pathogenic Yersinia enterocolitica using the multiplex polymerase chain

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Pathogenicity of FtsK mutant of avian pathogenic Escherichia coli

References 1. Begg K.J., Dewar S.J., Donachie W.D.: A new Escherichia coli cell division gene, ftsK. J Bacteriol 1995, 177, 6211-6222. 2. Bigot S., Sivanathan V., Possoz C., Barre F.X., Cornet F.: FtsK, a literate chromosome segregation machine. Mol Microbiol 2007, 64, 1434-1441. 3. Chen X., Gao S., Wang X.Q., Jiao X.A., Liu X.F.: Identification of APEC genes expressed in vivo by selective capture of transcribed sequences. Acta Microbiol Sin 2007, 47, 407-412. 4. Dho M., Lafont J.P.: Adhesive

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Monitoring of Selected Genes in Campylobacter Jejuni and Campylobacter Coli Isolates from Domestic Animals

References 1. Andrzejewska M., Klawe J.J., Szczepańska B., Śpica D.: Occurrence of virulence genes among Campylobacter jejuni and Campylobacter coli isolates from domestic animals and children. Pol J Vet Sci 2011, 2 , 207-211. 2. Bang D.D., Scheutz F., Ahrens P., Pedersen K., Blom J., Madsen M.: Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers. J Med Microbiol 2001, 50 , 1087-1094. 3. Carvalho A.C., Ruiz-Palacios G

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Histopathological and cytopathological findings in minipigs infected with Streptococcus suis serotype 2

to increase susceptibility of piglets to challenge by Streptococcus suis type II. J Virol 2001, 75, 4889-4895. 6. Fittipaldi N., Sekizaki T., Takamatsu D., de la Cruz Domínguez- Punaro M., Harel J., Bui N.K., Vollmer W., Gottschalk M.: Significant contribution of the pgdA gene to the virulence of Streptococcus suis. Mol Microbiol 2008, 70, 1120-1135. 7. Glass-Kaastra S.K., Pearl D.L., Reid-Smith R., McEwen B., Slavic D., Fairles J., McEwen S.A.: Multiple-class antimicrobial resistance surveillance in swine Escherichia coli F4

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Genetic changes in pigeon paramyxovirus type-1 induced by serial passages in chickens and microscopic lesions caused by the virus in various avian hosts

Haas N., Braber M., Römer-Oberdörfer A., van den Elzen P., van der Marel P.: Newcastle disease virus (NDV) marker vaccine: an immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope. J Virol 2002, 76, 10138–10146. 23. Meng C., Qiu X., Yu S., Li C., Sun Y., Chen Z., Liu K., Zhang X., Tan L., Song C., Liu G., Ding C.: Evolution of Newcastle disease virus quasispecies diversity and enhanced virulence after passage through chicken air sacs. J Virol 2015, 90, 2052–2063. 24. Meulemans G., van den Berg T

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Genotypic Markers of Yersinia Enterocolitica O:9 Isolated from Cows Positive in Serological Examination for Bovine Brucellosis

by immunomagnetic separation, nested polymerase chain reactions and colorimetric detection of amplified DNA. Appl Environ Microbiol 1993, 59 , 2938-2944. 9. Kot B., Piechota M., Jakubczak A.: Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes. Pol J Vet Sci 2010, 13 , 13-19. 10. Kot B.: Chromosomally and plasmid-encoded virulence determinants of Y. enterocolitica. Medycyna Wet 2010, 66, 294-298. 11. Lubeck P.S., Skurnik M., Akrens

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Phenotypic and Genotypic Antimicrobial Resistance of Lactococcus Sp. Strains Isolated from Rainbow Trout (Oncorhynchus Mykiss)

PFGE analysis. Lett Appl Microbiol 2011, 53, 614-619. 27. Turker H, Yildirim A.B, Karakas F.P.: Sensitivity of bacteria isolated from fish to some medicinal plants. Turk J Fish Aquat Sc 2009, 9, 181-186. 28. Van T.T.H, Chin J, Chapman T, Tran L.T, Coloe P.J.: Safety of raw meat and shellfish in Vietnam: an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes. Int J Food Microbiol 2008, 124, 217-223. 29. Vendrell D, Balcázar J.L, Ruiz-Zarzuela I, Gironés O, Múzquiz J.L.: Lactococcus garvieae in fish: A review. Comp Immunol

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