. Plattel WJ, Van den Berg A, Alsada Z et al. Comparison of serial plasma galectin-1 sCD163 and sCD30 with plasma TARC levels as circulating biomarkers for response evaluation in classical Hodgkin lymphoma. Klin Pediatr 2014; 226: 0–07. https://doi.org/10.1055/s-0034-1371117 24. Reichel J, Chadburn A, Rubinstein PG et al. Flow sorting and exome sequencing reveal the onco genome of primary Hodgkin and Reed-Sternberg cells. Blood 2015; 125: 1061-72. https://doi.org/10.1182/blood-2014-11-610436 25. Roemer MG, Advani RH, Redd RA et al. Classical Hodgkin Lymphoma
Goran Marjanović and Olivera Simonović
Wichit Thaveekarn, Sunchai Payungporn, Narumol Pakmanee, Sunutcha Suntrarachun, Suchitra Khunsap and Suthidee Petsong
genomic extraction kit (QIAamp DNA Mini Kit, Qiagen). Construction of the library and sequencing were performed by the Biochemistry Department, Medicine Faculty, Chulalongkorn University, Thailand. The library was prepared using a NEBNext Ultra DNA Library Prep Kit for Illumina. Whole genome sequencing and assembly were analyzed using a HiSeq 2000 Illumina system (New England Biolabs). PCR and DNA sequencing of the RD16 region (Rv3405c) Genomic DNA from the working seed BCG (lot No. FB03012) was extracted using a genomic extraction kit (QIAamp DNA Mini Kit, Qiagen
Methee Sriprapun, Chalinee Laosakul, Sunisa Krajiw, Kesinee Arunyingmongkol, Padet Siriyasatien and Wanla Kulwichit
ranging from asymptomatic infection, dengue fever and, dengue hemorrhagic fever (DHF) to dengue shock syndrome [ 2 ]. Previous studies by our group and by others have shown that, during acute infection, DENV genome is detectable in blood, saliva, and urine samples [ 3 - 6 ]. In addition, concurrent infections with multiple serotypes can occur in both infected mosquitoes and humans [ 7 - 10 ]. Here, we present a case of dual DENV infections with DENV2 and DENV4 serotypes in the urine at a single time point. The ‘minor’ serotype disappeared in subsequent specimens
References 1. Bhaijee F., Bell D.: Entamoeba gingivalis in Acute Osteomyelitis of the Mandible. Case Reports in Medicine, Article ID 357301, 2011. 2. Brown T.A. (2002). Genomes, 2nd edition. Department of Biomolecular Sciences, UMIST, Manchester, UK. Oxford: Wiley- Liss. Chapter in a book: 3,11. 3. Derda M. et al.: Incidence of Entamoeba gingivalis in the oral cavity of students. Journal of stomatology, 64, 10: 784-795, 2011. 4. Dominguez R., Holmes K.C.: Actin structure and function. Annual
Adrian Jarzynski, Katarzyna Papiernik and Malgorzata Polz-Dacewicz
guardian of genome, Nature , 358: 15-16, 1992. 6. Lis P., Niczyj-Raucy M., Lis M.: The molecular basis of cancer and genetic methods of its diagnosis, Nat. J. (Opole) , 44: 92-119, 2011. 7. Nylander D.M, Dabelsteen E., Hall P.A.: The p53 molecule and its prognostic role in squamous cel carcinomas of the head and neck, J. Oral. Pathol. Med. , 29 (9): 413-425, 2000. 8. Rusin P., Markiewicz L., Majsterek I.: Genetic predeterminations of head and neck cancer, Post. Hig. Dos. , 62: 490-501, 2008. 9. Sznarkowska A., Olszewski R., Zawacka-Pankau J
Wasin Laohavinij and Apiwat Mutirangura
, including Student t , Pearson χ 2 , and Benjamini false discovery rate (FDR) tests, to identify the genes and the molecular pathways associated with the aging process and various types of exercise. The official names of significant genes and their molecular functions, and their molecular and cellular phenotypes, were evaluated by GeneCards, a human gene database maintained by the Crown Human Genome Center at the Weizmann Institute of Science ( http://www.genecards.org/ ). Methods Overall methodical framework is shown in Figure 1 . Figure 1 The overall
Agnieszka Łaba-Stefanek, Ewelina Dziwota and Marcin Olajossy
badania asocjacyjne całego genomu (GWAS) wniosły do psychiatrii?, 2013. 8. Bergen S.E., Petryshen T.L. Genome-wide association studiem (GWAS) of schizophrenia: does bigger lead to better results?, 2013. 9. Hosak L., Silhan P., Hosakowa J. Genomic copy number variations: A breakthrough in our knowledge on schizophrenia etiology?, 2012. 10. Kotlar A.V., Mercer K.B., Zwick M.E., Mulle J.G. New discoveries in schizophrenia genetics reveal neurobiological pathways: A review of recent findings, 2015 11. Gumerov V., Hegyi H. MicroRNA-derived network
Haifeng Wang, Delin Yang, Jiansong Wang and Hongyi Xu
Background: Metastasis of tumor implantation includes a series of processes from detachment from the primary tumor to formation of the implanted metastase. Tumor cells survival in urine is a necessary condition for metastasis. Adaptation to urine is essential for this.
Objective: Establish a urine-resistant cell sub-strain of human bladder cancer cell line (ET cell lines), and study different characteristics compared to parent cells.
Methods: EJ cell lines were cultured in nutrient medium. Urine-resistance cell sub-strain (EJ-U) was harvested after prolonged culture by gradually increasing the concentration of urine. Gen chip was used to detect the genome series of EJ and EJ-U and to analyze the difference of gene expression.
Results: EJ-U in urine had a higher survival rate after 24 hours in urine compared with EJ. The EJ-U had almost the same growth velocity with EJ, and they had the analogous growth curves. The time-duration for EJ-U to survive was longer than EJ in urine. In gene ontology analysis, 272 significant different genes were found.
Conclusion: EJ-U cell sub-strain was more adaptable than its parent cell lines EJ. The different genes may explain the reason why bladder cancer cells could survive for a long time in urine.
Mohammad A. Farraj, Gabi M. Abusada, Abed Alraoof M. Saleem, Ayyub Y. Joaidi, Raba M. Radad, Hiba N. Atrash, Israr N. Sabri and Tamer A. Essawi
Background: Neisseria gonorrohoeae is an exclusive human pathogen that primarily infects the urogenital epithelia. Infections caused by N. gonorrhoeae are considered the second major cause of sexually transmitted disease after Chlamydiae worldwide. Although the urethra and the uterine cervix serve as the initial sites for gonococcal infections in men and women, infection of the conjunctiva, pharynx, tendons, joints, as well as rectal mucosa are also reported. Objectives: The objectives of this study were to introduce molecular techniques such as polymerase chain reaction (PCR) to detect N. gonorrhoeae directly from endocervical swabs. In addition, it provides a picture of Neisseria gonorrohea infection among a sample of Palestinian women in West Bank. Methods: Two hundred and thirteen endocervical swabs were collected from sexually active married women with endocervical abnormalities attending healthcare clinics. DNA was extracted directly from the swabs and PCR was performed using specific primers targeting the orf1 region of the genome. Results: The results obtained indicated that the percentage of positive cases of N. gonorrhoeae among the women tested was 1.40%. Conclusion: Implementing guidelines for comprehensive screening of men and women with more sensitive tests may improve detection and management of sexually transmitted infections.
M. Krasteva, Sv Angelova and Zl Gospodinova
Breast cancer is the most frequent malignancy among women. Advances in breast cancer knowledge have deciphered the involvement of a number of tumor suppressor genes and proto-oncogenes in disease pathogenesis. These genes are part of the complex biochemical pathways, which enable cell cycle control and maintenance of genome integrity. Their function may be disrupted as a result of alterations in gene sequence or misregulation of gene expression including alterations in DNA methylation pattern. The present review summarizes the main findings on major breast cancer related genes BRCA1/2, p53, ATM, CHEK2, HER2, PIK3CA and their tumorigenic inactivation/activation. The potential clinical importance of these genes with respect to patients’ prognosis and therapy are also discussed. The possible implication of other putative breast cancer related genes is also outlined. The first elaborate data on the genetic and epigenetic status of the above mentioned genes concerning Bulgarian patients with the sporadic form of the disease are presented. The studies indicate for a characteristic mutational spectrum in some of the genes for the Bulgarian patients and specific correlation between the status of different genes and clinicopathological characteristics.