The paper deals with the research of structure and dynamic development of stands in National Nature Reservation Trčkov, located in Protected Landscape Area Orlické Mts. (Czech Republic). The research was carried out in spruce-beech stand with admixed fir and sycamore maple. Two permanent research plots (PRP 1 and PRP 2), for each with area of 0.25 hectare were chosen as an object of our research. FieldMap technology was used for field measurement. Compared PRP were characterized by the same typological, soil, phytocenology and stand parameters, but different method of protection against game damage. Results showed that deer is considered to be limiting factor for successful development of natural regeneration of autochthonous tree species. The tree species proportion of natural regeneration occurred in PRP 1 with fencing was sufficient, contrary to PRP 2 (outside fencing) owing to site and stand conditions. The results of biometric measurements and predicted simulation by Sibyla growth simulator showed multi-layer stand structure and productive spruce-beech stands with interspersed fir and sycamore maple. We also found depleted phase of regeneration according to frequency and tree species proportion, as well as beginning of grow up stadium, especially related to fir and sycamore proportion and/or beech and spruce. It is concluded that growth visualization and simulation confirmed the hypothesis about long-term effect of game to species, spatial and age structure and development of concerned autochthonous population.