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D. O. Olayemi, M. M. Onakpa and O. C. Jegede


The development of host resistance to anthelmintics and the increasing cost of commercial anthelmintics have encouraged the need for the in vitro anthelmintic evaluation of crude extract and fractions of Hymenodictyon pachyanta plant as alternative drugs against Haemonchus contortus. H. contortus is one of the most prevalent and highly pathogenic parasitic nematodes in small ruminant farming globally. H. pachyanta stem bark is a prospective plant used by the local and indigenous farmers of Nsukka, Enugu state, Nigeria. The stem bark of H. pachyanta were collected, dried, pulverized and extracted with 80 % methanol. The purpose of this study was to investigate the in vitro anthelmintic effects of these crude extract and fractions against H. contortus in sheep and goats. The two extracts (crude and fractions) of H. pachyanta were tested by the egg hatch assay (EHA) and the larval development inhibition assays (LDIA) and to compared the results with albendazole (as the positive control). The concentrations for the crude extract and albendazole used for this study were 0.78, 1.56, 3.125, 6.25 and 12.5−1. The results demonstrated that the crude extracts, fractions and albendazole all at the concentration doses of 12.5−1 produced 100 % inhibition of egg hatching and larval development. Statistically, there was no significant difference (P > 0.05) in the mean percentage inhibition of egg hatching and larval development inhibition of the crude extracts and fractions when compared with albendazole. However, a significant difference (P < 0.05) was observed with n-butanol fraction which inhibited 96.17 % of egg hatchability. All of the extracts and albendazole showed ovicidal and larvicidal effects and were able to induce over 50 % of the egg hatching and mortality of larvae at the concentration ranges of 0.78—12.5−1. The results obtained from our study suggest that H. pachyanta had ovicidal and larvicidal activity against H. contortus and that the bioactive plants compounds responsible for this effect could be attributed to the presence of tannins, alkaloids and the saponins contained in the crude extracts.

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Magdalena Kulus, Małgorzata Józkowiak, Jakub Kulus, Małgorzata Popis, Blanka Borowiec, Katarzyna Stefańska, Piotr Celichowski, Mariusz J. Nawrocki, Dorota Bukowska, Klaus P. Brüssow, Bartosz Kempisty, Michal Jeseta and Paweł Antosik

Hematopoietic Stem Cell Self-Renewal and Stress Hematopoiesis via Repression of Cdkn2a. Stem Cells. 2017;35(8):1948–57; DOI: 10.1002/stem.2648. 19. Migueles RP, Shaw L, Rodrigues NP, May G, Henseleit K, Anderson KGV, et al. Transcriptional regulation of Hhex in hematopoiesis and hematopoietic stem cell ontogeny. Dev Biol. 2017;424(2):236–45; DOI: 10.1016/j.ydbio.2016.12.021. 20. Holets LM, Hunt JS, Petroff MG. Trophoblast CD274 (B7-H1) Is Differentially Expressed Across Gestation: Influence of Oxygen Concentration1. Biol Reprod. 2006;74(2):352–8; DOI: 10

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Wiesława Kranc, Maurycy Jankowski, Joanna Budna, Piotr Celichowski, Ronza Khozmi, Artur Bryja, Sylwia Borys, Marta Dyszkiewicz-Konwińska, Michal Jeseta, Magdalena Magas, Dorota Bukowska, Paweł Antosik, Klaus P. Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel and Bartosz Kempisty

References 1. Kranc, W. et al. , “The origin, in vitro differentiation, and stemness specificity of progenitor cells.,” J. Biol. Regul. Homeost. Agents , vol. 31, no. 2, pp. 365-369, 2017. 2. Groth, C. G., “The potential advantages of transplanting organs from pig to man: A transplant Surgeon’s view.,” Indian J. Urol. , vol. 23, no. 3, pp. 305-9, Jul. 2007. 3. Denner, J., “Xenotransplantation-Progress and Problems: A Review,” J. Transplant. Technol. Res. , vol. 4, no. 2, Jul. 2014. 4. Kranc, W. et al. , “Molecular basis of growth

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Ying Liu, Qi-Zhong Fu, Lin Pu, Qing-Guo Meng, Xian-Feng Liu, Sheng-Fang Dong, Jian-Xun Yang and Guang-Yao Lv

-11. 13. Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity. Cancer Res 2004; 64: 2024-9. 14. Fehr A, Meyer A, Heldorn K, Röser K, Löning T, Buller - diek J. A link between the expression of the stem cell marker HMGh2, grading, and the fusion CRTCl-MAML2 in mucoepidermoid carcinoma. Genes Chrom Cancer 2009; 48: 777-85. 15. De Martino I, Visone R, Wierinckx A, Palmieri D, Ferraro A, Cappabianca P, et al. HMGA proteins up-regulate CCNB2 gene in

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Agnieszka Knopik-Skrocka, Patrycja Kręplewska and Donata Jarmołowska-Jurczyszyn

References [1] Ailles LE, Weissman IL. Cancer stem cells in solid tumors. Curr Opinion Biotech 2007; 18: 460-466. [2] Alameddine RS, Hamich L, Shamseddine A. From sprouting angiogenesis to erythrocytes generation by cancer stem cells: evolving concepts in tumor microcirculation. BioMed Res Int 2014; 2014: doi 10.1155/2014/986768. [3] Asahara T, Masuda H, Takahashi T, Kalka C, Pastore C, Silver M. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and

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Marius Harpa, Ionela Movileanu, Leslie Sierad, Ovidiu Cotoi, Horațiu Suciu, Terezia Preda, Dan Nistor, Carmen Sircuța, Klara Brânzaniuc, Radu Deac, Simona Gurzu, Lucian Harceaga, Peter Olah, Dan Simionescu, Michael Dandel and Agneta Simionescu

Valve Scaffolds Obtained by Complete Decellularization of Porcine Aortic Roots in a Novel Differential Pressure Gradient Perfusion System. Tissue Eng Part C Methods. 2015 Dec;21(12):1284-96. DOI: 10.1089/ten.tec.2015.0170. 6. Gimble J, Guilak F. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003;5(5):362-9. DOI: 10.1080/14653240310003026. 7. Schoen FJ. Heart valve tissue engineering: quo vadis? Curr Opin Biotechnol. 2011 Oct;22(5):698-705. DOI: 10.1016/j.copbio.2011

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Katarzyna Stefańska, Agata Chamier-Gliszczyńska, Maurycy Jankowski, Piotr Celichowski, Magdalena Kulus, Magdalena Rojewska, Paweł Antosik, Dorota Bukowska, Małgorzata Bruska, Michał Nowicki, Bartosz Kempisty, Michal Jeseta and Jana Zakova

Domest Anim. 2008;43(1):66-73; DOI:10.1111/j.1439-0531.2007.00856.x. 11. White KL, Hehnke K, Rickords LF, Southern LL, Thompson DL Jr, Wood TC. Early embryonic development in vitro by coculture with oviductal epithelial cells in pigs. Biol Reprod. 1989;41(3):425-30. 12. Kessler M, Hoffmann K, Brinkmann V, Thieck O, Jackisch S, Toelle B, Berger H, Mollenkopf HJ, Mangler M, Sehouli J, Fotopoulou C, Meyer TF. The Notch and Wnt pathways regulate stemness and differentiation in human fallopian tube organoids. Nat Commun. 2015;6:8989; DOI:10.1038/ncomms9989

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Marijana Dajak

for multiple myeloma. J Clin Oncol 2005; 23 : 3412-20. Singhal S, Mehta J. Multiple myeloma. Clin J Am Soc Nephrol 2006; 1 : 1322-30. Bjorkstrand BB, Ljungman P, Svensson H, Hermans J, Alegre A, Apperley J, et al. Allogeneic bone marrow transplantation versus autologous stem cell transplatation in multiple myeloma: a retrospective case-matched study from the European Group for Blood and Marrow Transplantation. Blood 1996; 88 : 4711-8. Child JA, Morgan GJ, Davies FE, Owen RG, Bell

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Swaminathan Ganesh, Mala Dharmalingam and Sara Marcus

. Most free-radical injury is iron-related: it is promoted by iron, hemin, holoferritin and Vitamin C, and inhibited by desferoxamine and apoferritin. Stem Cells 1994; 12: 289-303. Mojiminiyi OA, Marouf R, Abdella NA. Body iron stores in relation to the metabolic syndrome, glycemic control and complications in female patients with type 2 diabetes. Nutr Metab Cardiovasc Dis 2008; 18: 559-66. Torti FM, Torti SV. Regulation of ferritin genes and protein. Blood 2002; 99: 3505-16. Baccin AC

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Ludwik K. Malendowicz and Andrzej Łukaszyk

CE. The origin of the epicardium and the embryonic myocardial circulation in the mouse. Anatomical Rec. 1981;201:157-168. DOI: 10.1002/ar.1092010117 15. Beltrami AP, Barlucchi L, Torella D, Baker M, Limana F, Chimenti S, Kasahara H, Rota M, Musso E, Urbanek K, Leri A, et al. Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell 2003;114:763–776. 16. Shiozaki M. Shimada Y. Intercellular junctions in embryonic chick cardiac muscle revealed by rapid freezing and freeze-substitution. Microscopy Res Tech. 1992