retentate) and continuous magnetic stirring at 90ºC for 24 hours. After cooling and monitoring completeness of hydrolysis, the pH-value of the hydrolysis cocktail was adjusted to 7.0 by adding an adequate quantity of aqueous NaOH solution.
Microbial growth of H. pseudoflava was monitored during the experiments by measuring the optical density at a wavelength of 420 nm using a Spectronic genesys 2 PC UV/VIS spectrophotometer after each sampling; for each measurement, filtrated supernatant was used as zero-reference.
. This pattern of synchronized accumulation of proline and H 2 O 2 due to Zn stress has also been reported in Citrus reticulate ( 36 ). To fully understand the physiology of osmoregulation mechanisms in A. orientalis , changes not only in proline but also in soluble sugars, sugar alcohols, and glycine betaine should also be considered.
MDA is frequently used as a biomarker of ROS-mediated cellular damage after oxidative stress ( 33 , 37 ). An increase in MDA content indicates increased peroxidation of cell membrane lipids, and MDA measurement provides a means of
fractions in biomass reached 0.20 and 0.27 g/g, when comparing two parallel bioreactor cultivations. The produced mcl -PHA mainly consisted of 3HO and 3HD building blocks in addition to minor amounts of the monomers 3HDD, 3-hydroxynonanoate (3HN), 3-hydroxyhexanoate (3HHx), and 3-hydroxyheptanoate (3HHp). Using NMR, the presence of traces of unsaturated and, surprisingly, 3HV monomers in these polyesters was also confirmed. Thermoanalysis measurement reported values typical for mcl -PHA, with T m of 48.6 °C and 53.6 °C, T g of -46.9 °C and -43.5 °C, X c of 12
Erdem Budak, Faruk Beytar, Mertcan Özdemir, Beyza Nur Susam, Meriç Göker, Aytekin Ünlü and Osman Eroğul
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Niyazi Acer, Nazan Dolu, Gokmen Zararsiz, Mehmet Sait Dogan, Kazim Gumus, Sevgi Ozmen, Ali Yucel Kara, Handan Soysal, Huseyin Per and Mehmet Bilgen
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