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An LC-MS Assay with Isocratic Separation and On-Line Solid Phase Extraction to Improve the Routine Therapeutic Drug Monitoring of Busulfan in Plasma

References 1. Lucarelli G, Isgro A, Sodani P, Gaziev J. Hematopoietic stem cell transplantation in thalassemia and sickle cell anemia. Cold Spring Harb Perspect Med 2012; 2: a011825. 2. Russell JA, Kangarloo SB. Therapeutic drug monitoring of busulfan in transplantation. Curr Pharm Des 2008; 14: 1936-49. 3. Iwamoto T, Hiraku Y, Oikawa S, Mizutani H, Kojima M, Kawanishi S. DNA intrastrand cross-link at the 5’-GA-3’ sequence formed by busulfan and its role in the cytotoxic effect. Cancer Sci 2004; 95: 454

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Science at the Crossroads: Fact or Fiction?

-8. Ghosh P. Journal stem cell work ‘blocked’. BBC 2010; February 2. ( Garfield E. Demand citation vigilance. Scientist 2002; 16: 6-7. Goldberg DM. Is scientific publishing a criminal activity? Clin Biochem 2006; 39: 473-81. Roll-Hansen N. The Lysenko effect: undermining the autonomy of science. Endeavour 2005; 29: 143-7. Monbiot G. Climate change email scandal

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Vesicular Arbuscular Mycorrhiza Influences the Histo-Anatomic Characteristics of Vegetative Organs in Artemisia annua


Recent studies have shown that vesicular-arbuscular mycorrhizae stimulate plant growth in case of Artemisia annua plants. According to these studies mycorrhization can enhance plant height and biomasses, shoot branching and inter-nodal length, foliar glandular hair density, and nutrient status of shoots and leafs. Contradictory data were obtained in case of leaf chlorophyll content and photosynthetic rate. The effects of vesicular-arbuscular mycorrhizae on roots, shoots and leafs anatomy of A. annua have not been studied yet. The aim of this paper was to compare the microscopic characteristics of the vegetative organs from the Artemisia annua plants treated with vesicular-arbuscular mycorrhizae, with those from the control plants. Rhizophagus irregularis influenced the development of vascular tissues in root and stem of Artemisia plants by increasing their surface in the organs. Mycorrhization also reduced the percentage of lignification in the cortex of the root, increased the percentage of palisade parenchyma in leaf and had a positive effect on foliar glandular hair density. Further investigations are necessary to find out the role of these histo-anatomic alterations in the growth and development of Artemisia plants.

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The differentiation and transdifferentiation of epithelial cells in vitro – is it a new strategy in regenerative biomedicine?

References 1. Gargett, C. E. and Masuda, H., “Adult stem cells in the endometrium,” Mol. Hum. Reprod. , vol. 16, no. 11, pp. 818-834, Nov. 2010. 2. Kranc, W. et al. , “The origin, in vitro differentiation, and stemness specificity of progenitor cells.,” J. Biol. Regul. Homeost. Agents , vol. 31, no. 2, pp. 365-369, 2017. 3. Kossowska-Tomaszczuk, K. et al. , “The Multipotency of Luteinizing Granulosa Cells Collected from Mature Ovarian Follicles,” Stem Cells , vol. 27, no. 1, pp. 210-219, Jan. 2009. 4. Marx, V., “Stem cells: a dish of

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Does migrative and proliferative capability of epithelial cells reflect cellular developmental competence?

.,” Am. J. Obstet. Gynecol. , vol. 133, no. 8, pp. 859-67, Apr. 1979. 21. Chan, R. W. S., Schwab, K. E., and Gargett, C. E., “Clonogenicity of Human Endometrial Epithelial and Stromal Cells,” Biol. Reprod. , vol. 70, no. 6, pp. 1738-1750, Jun. 2004. 22. Padykula, H. A., “Regeneration in the primate uterus: the role of stem cells.,” Ann. N. Y. Acad. Sci. , vol. 622, pp. 47-56, 1991. 23. Uduwela, A. S., Perera, M. A., Aiqing, L., and Fraser, I. S., “Endometrial-myometrial interface: relationship to adenomyosis and changes in pregnancy.,” Obstet

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Tyreoliberin (Trh) – The Regulatory Neuropeptide of Cns Homeostasis


The physiological role of thyreoliberin (TRH) is the preservation of homeostasis within four systems (i) the hypothalamic-hypophsysiotropic neuroendocrine system, (ii) the brain stem/midbrain/spinal cord system, (iii) the limbic/cortical system, and (iv) the chronobiological system. Thus TRH, via various cellular mechanisms, regulates a wide range of biological processes (arousal, sleep, learning, locomotive activity, mood) and possesses the potential for unique and widespread applications for treatment of human illnesses. Since the therapeutic potential of TRH is limited by its pharmacological profile (enzymatic instability, short half-life, undesirable effects), several synthetic analogues of TRH were constructed and studied in mono- or adjunct therapy of central nervous system (CNS) disturbances. The present article summarizes the current state of understanding of the physiological role of TRH and describes its putative role in clinical indications in CNS maladies with a focus on the action of TRH analogues.

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“Cell cycle process”, “cell division” and “cell proliferation” belong to ontology groups highly regulated during long–term culture of porcine oviductal epithelial cells

Hematopoietic Stem Cell Self-Renewal and Stress Hematopoiesis via Repression of Cdkn2a. Stem Cells. 2017;35(8):1948–57; DOI: 10.1002/stem.2648. 19. Migueles RP, Shaw L, Rodrigues NP, May G, Henseleit K, Anderson KGV, et al. Transcriptional regulation of Hhex in hematopoiesis and hematopoietic stem cell ontogeny. Dev Biol. 2017;424(2):236–45; DOI: 10.1016/j.ydbio.2016.12.021. 20. Holets LM, Hunt JS, Petroff MG. Trophoblast CD274 (B7-H1) Is Differentially Expressed Across Gestation: Influence of Oxygen Concentration1. Biol Reprod. 2006;74(2):352–8; DOI: 10

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Amino acids metabolism and degradation is regulated during porcine oviductal epithelial cells (OECs) primary culture in vitro – a signaling pathways activation approach

References 1. Kranc, W. et al. , “The origin, in vitro differentiation, and stemness specificity of progenitor cells.,” J. Biol. Regul. Homeost. Agents , vol. 31, no. 2, pp. 365-369, 2017. 2. Groth, C. G., “The potential advantages of transplanting organs from pig to man: A transplant Surgeon’s view.,” Indian J. Urol. , vol. 23, no. 3, pp. 305-9, Jul. 2007. 3. Denner, J., “Xenotransplantation-Progress and Problems: A Review,” J. Transplant. Technol. Res. , vol. 4, no. 2, Jul. 2014. 4. Kranc, W. et al. , “Molecular basis of growth

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Hmga2 Expression in Renal Carcinoma and its Clinical Significance / Ekspresija Hmga2 U Karcinomu Bubrega I Njen Klinički Značaj

-11. 13. Miyazawa J, Mitoro A, Kawashiri S, Chada KK, Imai K. Expression of mesenchyme-specific gene HMGA2 in squamous cell carcinomas of the oral cavity. Cancer Res 2004; 64: 2024-9. 14. Fehr A, Meyer A, Heldorn K, Röser K, Löning T, Buller - diek J. A link between the expression of the stem cell marker HMGh2, grading, and the fusion CRTCl-MAML2 in mucoepidermoid carcinoma. Genes Chrom Cancer 2009; 48: 777-85. 15. De Martino I, Visone R, Wierinckx A, Palmieri D, Ferraro A, Cappabianca P, et al. HMGA proteins up-regulate CCNB2 gene in

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Tumor Blood Vessels and Vasculogenic Mimicry – Current Knowledge and Searching for New Cellular/Molecular Targets of Anti-Angiogenic Therapy

References [1] Ailles LE, Weissman IL. Cancer stem cells in solid tumors. Curr Opinion Biotech 2007; 18: 460-466. [2] Alameddine RS, Hamich L, Shamseddine A. From sprouting angiogenesis to erythrocytes generation by cancer stem cells: evolving concepts in tumor microcirculation. BioMed Res Int 2014; 2014: doi 10.1155/2014/986768. [3] Asahara T, Masuda H, Takahashi T, Kalka C, Pastore C, Silver M. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and

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