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Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products

drying gas (air) flow, each 10 L/min, interface temperature at 300°C, desolvation line temperature at 250°C, heat block temperature at 400°C, and capillary voltage at −3 and 4 kV for negative and positive ionisation, respectively. The mass analyser was configured in selected reaction monitoring mode; each analyte was analysed using two transitions. The compounds were separated using a Kinetex F5 (100 mm, 2.1 mm, 2.6 μm) analytical column (Phenomenex, USA) and acetonitrile (A) and 10 mM ammonium acetate pH 7.0 (B) as a mobile phase. The following gradient elution was

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Plasma metabolic characterisation of dairy cows with inactive ovaries and oestrus during the peak of lactation

-nuclear magnetic resonance spectroscopy in postpartum dairy cows with ovarian inactivity. Theriogenology 2016, 86, 1475–1481. 27291083 10.1016/j.theriogenology.2016.05.005 Xu C. Xia C. Sun Y. Xiao X. Wang G. Fan Z. Shu S. Zhang H. Xu C. Yang W. Metabolic profiles using (1)H-nuclear magnetic resonance spectroscopy in postpartum dairy cows with ovarian inactivity Theriogenology 2016 86 1475 1481 29 Yudkoff M., Daikhin Y., Nissim I., Horyn O., Luhovyy B., Lazarow A., Nissim I.: Brain amino acid requirements and toxicity: the

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Phenotypic diversity and potential virulence factors of the Shewanella putrefaciens group isolated from freshwater fish

for 48 h at 27 ± 2°C on tryptic soy agar (TSA) supplemented with 0.0%, 0.5%, 2.0%, 3.0%, 4.0%, 5.0%, or 6.0% of NaCl. Molecular identification of the isolates . DNA extraction and 16S rRNA gene sequence analysis were performed as described previously ( 21 ). Sequences were deposited in the GenBank database under accession numbers KC607503, KC607506–KC607522, KC607524, KC607525, KC607527–KC607530, KY630559, KY817247, KY817248–KY817252, KY817255, KY817257, KY817258, MF958276, MF958964, MF959701, MF962592, MF962594– MF962596, MF962598, MG930082, and MG930083 and

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ARTs in wild felid conservation programmes in Poland and in the world

. Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration Theriogenology 2008 69 485 490 55 Zambelli D., Bini C., Kuster D.G., Molari V., Cunto M.: First deliveries after estrus induction using deslorelin and endoscopic transcervical insemination in the queen. Theriogenology 2015, 84, 773–778. 10.1016/j.theriogenology.2015.05.010 26092701 Zambelli D. Bini C. Kuster D.G. Molari V. Cunto M. First deliveries after estrus induction using deslorelin and

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The use of the water treadmill for the rehabilitation of musculoskeletal injuries in the sport horse

submerged in water. J Animal Physiol Anim Nutr (Berl) 2012, 96, 563–569. 10.1111/j.1439-0396.2011.01179.x Lindner A. Wäschle S. Sasse H.H.L. Physiological and blood biochemical variables in horses exercised on a treadmill submerged in water J Animal Physiol Anim Nutr (Berl) 2012 96 563 569 19 MacDermid P.W., Wharton J., Schill C., Fink P.W.: Water depth effects on impact loading, kinematic and physiological variables during water treadmill running. Gait Posture 2017, 56, 108–111. 10.1016/j.gaitpost.2017.05.013 28535432 MacDermid P

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Mouse (Mus musculus) embryonic cerebral cortex cell death caused by carbofuran insecticide exposure

washed using PBS or 150 mM KCl to remove erythrocytes. The washed tissue was homogenised with 0.1 M Tris/HCl (pH 7.4) containing 5 mM β-ME of 0.5% Triton X-100 and 0.1 mg/mL of phenylmethylsulphonyl fluoride (PMSF). The compound was centrifuged at 14,000 g at 4°C for 5 min. The debris of supernatant was removed to obtain cytolytic and mitochondrial SOD. The examination was conducted by putting 20 μL of sample into a sample cuvette and cuvette blank 2 and putting the same with addition of 20 μL H 2 O into cuvette blank 1 and cuvette blank 3. WST working solution in a

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Hyponatraemia and syndrome of inappropriate antidiuretic hormone secretion in non-azotaemic dogs with babesiosis associated with decreased arterial blood pressure

J Vet Res 2013 74 1542 1545 4 Baneth G., Florin-Christensen M., Cardoso L., Schnittger L.: Reclassification of Theileria annae as Babesia vulpes sp. nov. Parasit Vectors 2015, 8, 207. doi: 10.1186/s13071-015-0830-5. 10.1186/s13071-015-0830-5 Baneth G. Florin-Christensen M. Cardoso L. Schnittger L. Reclassification of Theileria annae as Babesia vulpes sp nov. Parasit Vectors 2015 8 207 10.1186/s13071-015-0830-5 5 Barrot A.C., Bédard A., Dunn M.: Syndrome of inappropriate antidiuretic hormone secretion in a dog with a

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Clinical chemistry investigations in recumbent and healthy German Holstein cows after the fifth day in milk

phospholipase C plays a key role in second messenger cascades after PTH has bound to its receptor and is crucial for their full activity. Maintenance of physiological blood Mg concentration is almost exclusively dependent on a constant influx from nutrition. Thus, partial or total anorexia in the cow is frequently accompanied by lower blood Mg values. Clinical signs of hypomagnesaemia such as recumbency combined with convulsions are only seen if blood Mg falls below 0.5 mmol/L ( 13 , 20 , 22 ). The hypocalcaemia observed in the cows of this study most likely arose

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Screening of early diagnostic markers of gentamicin-induced acute kidney injury in canines

.96 ± 5.46*** 148.56 ± 37.39 82.00 ± 4.41* 84 32.88 ± 3.63*** 67.49 ± 8.58*** 161.701 ± 9.77 87.00 ± 4.63* 90 33.36 ± 7.26*** 69.81 ± 8.73*** 151.85 ± 18.24 84.92 ± 8.84* 96 33.56 ± 7.80*** 85.95 ± 9.20*** 203.78 ± 43.31* 93.75 ± 8.31*** 102 37.89 ± 10.99*** 90.51 ± 10.84*** 204.86 ± 44.98* 115.08 ± 16.75*** 108 36.74 ± 3.39*** 113.89 ± 28.50*** 197.70 ± 55.87* 129.00 ± 19.42*** * P < 0.05, ** P < 0.01, and *** P < 0.001, compared with the levels before injection Histopathological

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Bivagina pagrosomi Murray (1931) (Monogenea: Polyopisthocotylea), a microcotylid infecting the gills of the gilt-head sea bream Sparus aurata (Sparidae) from the Red Sea: morphology and phylogeny

aquaticus (Taq) polymerase, 0.1 μg of extracted parasite genomic DNA, and nuclease-free sterile double-distilled water up to 50.0 μL. The mixture was then subjected to a precise thermal profile through 60 cycles in a programmable thermocycler (Biometra GmbH, Germany) as follows: at 94°C for 30 s, at 50°C for 1 min, and at 72°C for 2 min ( 4 ). The amplified product (10–15 μL) was confirmed by using agarose gel electrophoresis (1.5%) and the DNA bands were stained with ethidium bromide (0.5 μg/ml) against the GeneRuler 100 bp Plus ready-to-use DNA ladder as a molecular

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