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Composition of microbial PLFAs and correlations with topsoil characteristics in the rare active travertine spring-fed fen

Abstract

We studied soil PLFAs composition and specific soil properties among transect of small-scale fen in Stankovany, Slovakia. The aim of this study was to determine potential differences in the microbial community structure of the fen transect and reveal correlations among PLFAs and specific soil characteristics. PCA analyses of 43 PLFAs showed a separation of the samples along the axis largely influenced by i14:0, 16:1ω5, br17:0, 10Me16:0, cy17:0, cy17:1, br18:0 and 10Me17:0. We measured a high correlation of sample scores and distance from fen edge (Kendall’s test τ = 0.857, P < 0.01). Kendall’s test showed a negative correlation of PLFAs content (mol%) and distance from the fen border for Gram (+) bacteria, Actinomycetes, mid-chain branched saturated PLFAs and total PLFAs. The redundancy analysis of the PLFA data set for the eight samples using PLFAs as species and 21 environmental variables identified soil properties significantly associated with the PLFA variables, as tested by Monte Carlo permutation showing most significant environmental variables including dichlormethan extractables, water extractables, Klason lignin, acid-soluble lignin, holocellulose, total extractables, organic matter content, total PLFA amount, bacterial PLFA and total nitrogen negatively correlated to axis 1 and dry weight and carbonate carbon positively correlated to axis 1. The amounts of Klason lignin, acid-soluble lignin, holocellulose total extractables, total PLFA, bacterial PLFA and total nitrogen were significantly correlated positively to the distance from fen border while moisture and total carbonate carbon were correlated negatively.

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Landscape Capacity for Ecosystem Services Provision Based on Expert Knowledge and Public Perception (Case Study from the Northwest Slovakia)

Abstract

Landscape represents appropriate spatial dimension for a study of ecosystems, especially due to ability to translate scientific knowledge into proper guidance for land use practice and enhancing the inclusion of local stakeholders in decision-making procedures. We tested social preferences method to reach initial and raw overview of the ecosystem services (ES) distribution and their values in the study areas. Perception of experts and local residents about capacities of relevant CORINE land cover (CLC) types to provide various ES was linked with Geographic Information System databases. We quantified the results on the basis of the mean values for each CLC type and the ES groups and these were interpreted also in spatial context. The expectation about perceptible capacities of forest to provide goods and services was fulfilled by responses of the experts, as was the expected difficulty to assess capacities of transitional woodland shrub or complex cultivation patterns. However, additional land cover types in question are meadows and pastures or discon-tinuous urban fabric. Mostly middle ranking values prevail in responses of local residents and uncertainty in the background is much greater comparing to the experts. On the other hand, rural people may better recognise diversified fow of services due to their everyday close connection to more ES. Large variation in the scores of some valued CLC classes in responses of the local residents and also experts seems resulting from lack of knowledge in the background and differences in viewpoint and appreciation. We understand the gaps in evaluating ES by the experts and resident population as good experience and key challenge for the further steps and fine-tuning of the research methods.

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A Simultaneous Analytical Method to Profile Non-Volatile Components with Low Polarity Elucidating Differences Between Tobacco Leaves Using Atmospheric Pressure Chemical Ionization Mass Spectrometry Detection

Summary

A comprehensive analytical method using liquid chromatography atmospheric pressure chemical ionization mass spectrometry detector (LC/APCI-MSD) was developed to determine key non-volatile components with low polarity elucidating holistic difference among tobacco leaves. Nonaqueous reversed-phase chromatography (NARPC) using organic solvent ensured simultaneous separation of various components with low polarity in tobacco resin. Application of full-scan mode to APCI-MSD hyphenated with NARPC enabled simultaneous detection of numerous intense product ions given by APCI interface. Parameters for data processing to filter, feature and align peaks were adjusted in order to strike a balance between comprehensiveness and reproducibility in analysis. 63 types of components such as solanesols, chlorophylls, phytosterols, triacylglycerols, solanachromene and others were determined on total ion chromatograms according to authentic components, wavelength spectrum and mass spectrum. The whole area of identified entities among the ones detected on total ion chromatogram reached to over 60% and major entities among those identified showed favorable linearity of determination coefficient of over 0.99. The developed method and data processing procedure were therefore considered feasible for subsequent multivariate analysis. Data matrix consisting of a number of entities was then subjected to principal component analysis (PCA) and hierarchical clustering analysis. Cultivars of tobacco leaves were distributed far from each cultivar on PCA score plot and each cluster seemed to be characterized by identified non-volatile components with low polarity. While fluecured Virginia (FCV) was loaded by solanachromene, phytosterol esters and triacylglycerols, free phytosterols and chlorophylls loaded Burley (BLY) and Oriental (ORI) respectively. Consequently the whole methodology consisting of comprehensive method and data processing procedure proved useful to determine key-components among cultivars of tobacco leaves, and was expected to additionally expand coverage that metabolomics study has ensured. [Beitr. Tabakforsch. Int. 27 (2016) 60-73]

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Effect of Environmental Disturbances on Odonata Assemblages along a Tropical Polluted River

Abstract

Odonata larvae have been intensively used as bioindicators for freshwater pollution as their community structure closely follow changes in the environment and habitat settings. In this study, 28 taxa of Odonata larvae were collected from three stations (upper, middle and lower) of a polluted river in Malaysia. The upper river basin receives effluents from an oil palm plantation. However, the middle station is presumably contaminated with anthropogenic wastes. The lower station is found to receive polluted discharges from aquaculture outlet. Several environmental parameters of water and sediment were continuously measured during the study. The water parameters showed no significant differences amongst the three stations. The species richness of Odonata was 22, 24 and 20 in the upper, middle and lower stations, respectively. The abundance of Odonata was significantly different among the studied sites. The tolerant damselfly, Pseudagrion sp. (41.22%), and facultative dragonflies, Onychothemis sp. (17.12%), were the most dominant taxa along the river stations. Onychothemis sp. and Paragomphus capricornis were equally important at the upper station [Important Species Index (ISI) 25.3 and 24.2%, respectively]. Pseudagrion sp. only scored an ISI value of 9.7%. Pseudagrion sp., P. capricornis and Onychothemis sp. were dominant in the middle station (ISI: 41.2%, 25.9% and 10.9% respectively), and Pseudagrion sp., Onychothemis sp. and Prodasineura sp. dominated the areas with dense growth of submerged aquatic weeds Hydrilla sp. in the lower station (ISI: 47.9, 24.5 and 13.8%, respectively). On the basis of the variations in larval abundance and ISI values, microhabitats differences partly in response to different types of pollutions entering the water structured the Odonata communities in this river basin.

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Histomorphometric Evaluation of Organic Enamel Elements in Erupted Bovine Teeth. Part I. Enamel Tufts

-308. 4. Braga M.M., Martignon S., Ekstrand K.R., Ricketts D.N., Imparato J.C., Mendes F.M.: Parameters associated with active caries lesions assessed by two different visual scoring systems on occlusal surfaces of primary molars - a multilevel approach. Community Dent Oral Epidemiol 2010, 38 , 549-558. 5. Emily P., Orsini P.G., Lobprise H.B., Wiggs R.B.: Oral and dental disease in large animals. In: Veterinary Dentistry Principles & Practice edited by Wiggs R.B., Lobprise H.B., Lippincott-Raven Publishers, Philadelphia, 1997, pp. 576

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The fine structures of Cystocaulus ocreatus (Nematoda: Protostrongylidae) and the related lung pathology

(Protostronglylidae, Nematoda) using macroscopic lesion scores. Vet. Res., 28: 143–148 [4] Guttekova, A., Zmoray, I. (1974): Comparative study of the ultrastructure of muscle cells in pneumohelminths. Helminthologia, 15: 585–602 [5] Hernandez de Lujan, S., Muela De la, N., Ferre, I. (2001): Helminth parasites in goats in Valencia region. Med. Vet., 18: 476–482 [6] Levine, N. D. (1968): Nematode Parasites of Domestic Animals and of Man. Burgess Publishing Company, USA. [7

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Evaluation of the suitability of Festuca rubra L. and F. nigrescens Lam. ecotypes as a material for lawn grass breeding

Abstract

In the years 2004-2006, 37 F. rubra L. ecotypes and 35 F. nigrescens Lam. ecotypes were evaluated for their main lawn traits: the general aspect of the plant, slow re-growth, overwintering, winter greenness, leaf fineness and disease resistance. The lawn properties of the ecotypes were assessed with the use of the IHAR 9-grade scale of the visual quality classification system. The study individuals were compared with the model varieties: F. rubra ‘Areta’ and F. nigrescens ‘Nimba’. The ecotypes originated from natural localities in the Lublin region. The experiment was conducted using the method of randomly chosen blocks in three repetitions. One repetition contained six plants of one ecotype grown at a distance of 75 × 30 cm. The aim of the study was to analyze the variability of lawn traits in the examined F. rubra and F. nigrescens ecotypes and to estimate the suitability of the selected material for the breeding of new lawn varieties. Analyses indicated that most of the ecotypes that grow in natural localities in the Lublin region display high-grade lawn traits. This confirms the great suitability of the wild plants for further breeding. Ecotypes of both species obtained high scores comparable with model varieties for their disease resistance, leaf fineness and winter greenness, and also for general aspect and slow re-growth.

Open access
Study on the quality of wines produced from ‘Syrah’ and ‘Tempranillo’ cultivars planted in two microregions in Southern Bulgaria

Study on the quality of wines produced from ‘Syrah’ and ‘Tempranillo’ cultivars planted in two microregions in Southern Bulgaria

In the period 2006-2008 a comparative study was carried out on the ‘Tempranillo’, ‘Syrah 99’ and ‘Syrah 100’ cultivars, grown in two microregions in Southern Bulgaria - Brestnik and Pesnopoy. The soils in the region of Pesnopoy are of a better mechanical and chemical composition, which preconditions the formation of relatively high yields of good quality grapes. The ‘Tempranillo’ cultivar was highly productive in both microregions, however the quality of the wines was lower, compared to the ‘Syrah’ cultivar. They were poorer both in the content of extract and in fruit aroma. ‘Syrah 99’ from Brestnik and ‘Syrah 100’ clones from Pesnopoy do not differ significantly in productivity, but the wines of ‘Syrah 100’ from the Pesnopoy region were more intense in colour and their aroma was strong, full-bodied and lasting with dominating floral (violet) and forest fruit nuances. Tasting results showed high scores, which is evidence that the young wines have potential and contain the ingredients and components necessary to develop further into top quality red wines.

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Identification of new superwarfarin-type rodenticides by structural similarity. The docking of ligands on the vitamin K epoxide reductase enzyme’s active site

: automated docking with selective receptor flexibility. Journal of Computational Chemistry 16, 2785–2791. [5] Trott, O., Olson, A. J. (2010), AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading. Journal of Computational Chemistry 31, 455–461. [6] O’Boyle Noel, M., Banck, M., James Craig, A., Morley, C., Vandermeersch, T., Hutchison Geoffrey, R. (2011), Open Babel: An open chemical toolbox. J. Cheminf . 3, 33–47. [7] National Center for Biotechnology Information. PubChem

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Effectiveness of obstetric procedures in miniature dogs

1994, 35 , 402-407. 4. Evans K.M., Adams V.J.: Proportion of litters of purebred dogs born by caesarean section. J Small Anim Pract 2010, 51 , 113-118. 5. Funkquist P.M., Nyman G.C., Löfgren A.J., Fahlbrink E.M.: Use of propofol-isoflurane as an anesthetic regimen for cesarean section in dogs. J Am Vet Med Assoc 1997, 211 , 313-317. 6. Groppetti D., Pecile A., Del Carro A.P., Copley K., Minero M., Cremonesi F.: Evaluation of newborn canine viability by means of umbilical vein lactate measurement, apgar score and

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