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Influence of the genetic makeup of common carp on the expression of iron-related genes during Trypanoplasma borreli infection

to a reaction mix, containing 12.5 μL of GoTaq qPCR Master Mix 2x, 5.5 μL of nuclease-free H 2 O, and 1 μL (concentration 10 μM) of each primer, making a total volume of 25 μL per reaction. Each sample was prepared in duplicate. A non-template control was included for each set of primers. The following cycling profile was applied: 50°C for 2 min, 95°C for 10 min, 40 cycles of 95°C for 15 s, 60°C for 1 min, and 72°C for 30 s, with the final holding stage at 60°C for 1 min. As a control, the 40 S ribosomal protein S11 gene was used. The comparative 2 -ΔΔCt method

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Xylazine regulates the release of glycine and aspartic acid in rat brain

/sugar mixture for 10 min and placed in 1 mL of 0.1% (v/v) Triton X-100 for 10 min at room temperature. After two washes with phosphate-buffered saline (PBS), the cells were incubated in 10% foetal bovine serum (Invitrogen, USA) for 1 h. Then the cells were incubated overnight at 4°C with a mouse anti-MAP2 antibody (Invitrogen, USA), followed by goat anti-mouse IgG (1:100; Santa Cruz Biotechnology, USA) for 1 h and washed three times with PBS. Treatment management Neurons harvested from foetal rats were treated with one of the following xylazine concentrations: 15 μg

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The Effect of Peroral Administration of Lactobacillus Fermentum Culture on Dairy Cows Health Indices

amines in the rumen of dairy cows. Livestock Science 155(2-3): 262-272. 4. Čejna, V., Chládek, G. (2005). The importance of monitoring changes in milk fat to milk protein ratio in holstein cows during lactation. Journal of Central European Agriculture 6(4): 539-546. 5. Muralithas, M., Shakthevale, A., Pushpakumara, P.G.A. (2011). Herd-based diagnosis of subacute ruminal acidosis (SARA) and disease investigation of the lameness at New Zealand dairy farm in Sri Lanka. Proceedings of the 16th International

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Ecological Factors of Transmission, Persistence and Circulation of Pathogens In Bat Populations

REFERENCES 1. Adesiyun, A. A., Stewart-Johnson, A., Thompson, N. N., 2009: Isolation of enteric pathogens from bats in Trinidad. J. Wildl. Dis. , 45 (4), 952—961. DOI: 10.7589/0090-3558-45.4.952. 2. Allocati, N., Petrucci, A. G., Di Giovanni, P., Masulli, M., Di Illio, C., De Laurenzi, V., 2016: Bat-man disease transmission: zoonotic pathogens from wildlife reservoirs to human populations. In Cell Death Discovery 2 , Article No. 16048. . 3. Bartlett, M., 1957: Measles periodicity and

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Development and Validation of Confirmatory Method for Analysis of Nitrofuran Metabolites in Milk, Honey, Poultry Meat and Fish by Liquid Chromatography-Mass Spectrometry

powder based on the use of liquid chromatography tandem mass spectrometry Analytica Chimica Acta, 572, 113–120. PMid:17723467 13. Commission Decision 2003/181/EC, 13 March 2003, amending decision 2002/657/EC as regards the setting of minimum performance limits (MRPLs) for certain residues in food animal origin, Off. J. Eur. Union, L71/17, 2003.

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Influence of hydrogen-rich saline on hepatocyte autophagy during laparoscopic liver ischaemia-reperfusion combined resection injury in miniature pigs

pig was pre-medicated subcutaneously with 0.05 mg/kg of atropine (Beijing Shuanghe Pharmaceutical, China) and 15 min later intramuscular injections of xylazine (1 mg/kg) and ketamine hydrochloride (10 mg/kg) were given and intravenous administration of propofol (3.5 mg/kg) was used to induce anaesthesia. The animal was intubated with a 6.0–7.0 mm tracheal tube and maintained with 1.5%–3% isoflurane in oxygen after intubation. Laparoscopic hepatic ischaemia-reperfusion and partial resection were performed after establishing a 4-portal approach. After laparoscopic

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Morphology, Morphometry and Histogenesis of the Prenatal Dromedary (Camelus dromedarius) Spleen

). Histomorphometric study of the prenatal development of the circumvallate papillae of one-humped camel (Camelus dromedarius). Anat Physiol. 5, 1. 7. Maina, M.M., Usende, I.L., Igwenagu, E., Onyiche, T.E., Yusuf, Z.M., Ntung, N.O. (2014). Gross, histological and histomorphometric studies on the spleen of one humped camel (Camelus dromedarius) found in the semi-arid region of North Eastern Nigeria. J. Vet. Adv. 4 (10): 703-711. 8. Jaji, A.Z., Kwari, H.D., Ribadu, A.Y., Sivachelvan, M

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In vitro cultivation and immunostaining of Lawsonia intracellularis strains

. Vet Microbiol 2003, 91, 135-145. 6. Huerta B., Arenas A., Carrasco L., Maldonado A., Tarradas C., Carbonero A., Perea A.: Comparison of diagnostic techniques for porcine proliferative enteropathy ( Lawsonia intracellularis infection). J Comp Pathol 2003, 129, 179-185. 7. Hwang J.M., Lee J.H., Yeh J.Y.: A multi-laboratory profile of Mycoplasma contamination in Lawsonia intracellularis cultures. BMC Res Notes 2012, 5, 78, doi: 10.1186/1756-0500-5-78. 8. Jacobson M., Fellström C., Jensen-Warn M.: Porcine

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Clostridium perfringens spores in Polish honey samples

agar (FAA) medium plates. The first of these media comprised peptic digest of animal tissue 10 g/L, meat extract 10 g/L, sodium chloride 5 g/L, lactose 12 g/L, neutral red 0.032 g/L, and agar 10 g/L, with final pH 7.0 ± 0.2 at 25°C and the second peptone 23 g/L, sodium chloride 5 g/L, soluble starch 1 g/L, sodium bicarbonate 0.4 g/L, glucose 1 g/L, sodium pyruvate 1 g/L, L-cysteine HCl×H 2 O 0.5 g/L, sodium pyrophosphate 0.25 g/L, L-arginine 1 g/L, sodium succinate 0.5 g/L, Hemin 0.01 g/L, vitamin K 0.001 g/L, and agar 12 g/L, with final pH 7.2 ±0.2 at 25°C. The

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Microbiological Analysis of Dried Goose Carcasses

). Goose. Goose breding station report. TKV Publication, Ankara. 5. Gulbaz, G., Kamber, U. (2008). Experimentally fermented sausage from goose meat and quality attributes. J Muscle Foods, 19(3): 247–260. 6. Anon. (2014). Centers for disease control and prevention. Web page statistics , Access date: 06.05.2014. 7. Anon. (2013). Surveillance for foodborne disease outbreaks–United States, 1998–2008. Centers for disease. Control and Prevention

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