Teresa Kamińska-Gibas, Ilgiz Irnazarow, Joanna Szczygieł and Patrycja Jurecka
to a reaction mix, containing 12.5 μL of GoTaq qPCR Master Mix 2x, 5.5 μL of nuclease-free H 2 O, and 1 μL (concentration 10 μM) of each primer, making a total volume of 25 μL per reaction. Each sample was prepared in duplicate. A non-template control was included for each set of primers. The following cycling profile was applied: 50°C for 2 min, 95°C for 10 min, 40 cycles of 95°C for 15 s, 60°C for 1 min, and 72°C for 30 s, with the final holding stage at 60°C for 1 min. As a control, the 40 S ribosomal protein S11 gene was used. The comparative 2 -ΔΔCt method
Yi-Ming Zhang, Dong-Xu Yu, Bai-Shuang Yin, Xin-Ran Li, Li-Na Li, Ya-Nan Li, Yu-Xin Wang, Yu Chen, Wen-Han Liu and Li Gao
/sugar mixture for 10 min and placed in 1 mL of 0.1% (v/v) Triton X-100 for 10 min at room temperature. After two washes with phosphate-buffered saline (PBS), the cells were incubated in 10% foetal bovine serum (Invitrogen, USA) for 1 h. Then the cells were incubated overnight at 4°C with a mouse anti-MAP2 antibody (Invitrogen, USA), followed by goat anti-mouse IgG (1:100; Santa Cruz Biotechnology, USA) for 1 h and washed three times with PBS.
Neurons harvested from foetal rats were treated with one of the following xylazine concentrations: 15 μg
amines in the rumen of dairy cows. Livestock Science 155(2-3): 262-272. https://doi.org/10.1016/j.livsci.2013.05.026
4. Čejna, V., Chládek, G. (2005). The importance of monitoring changes in milk fat to milk protein ratio in holstein cows during lactation. Journal of Central European Agriculture 6(4): 539-546.
5. Muralithas, M., Shakthevale, A., Pushpakumara, P.G.A. (2011). Herd-based diagnosis of subacute ruminal acidosis (SARA) and disease investigation of the lameness at New Zealand dairy farm in Sri Lanka. Proceedings of the 16th International
S. Zemanová, Ľ. Korytár, Z. Benkő, M. Prokeš and A. Ondrejková
1. Adesiyun, A. A., Stewart-Johnson, A., Thompson, N. N., 2009: Isolation of enteric pathogens from bats in Trinidad. J. Wildl. Dis. , 45 (4), 952—961. DOI: 10.7589/0090-3558-45.4.952.
2. Allocati, N., Petrucci, A. G., Di Giovanni, P., Masulli, M., Di Illio, C., De Laurenzi, V., 2016: Bat-man disease transmission: zoonotic pathogens from wildlife reservoirs to human populations. In Cell Death Discovery 2 , Article No. 16048. https://www.nature.com/articles/cddiscovery201648 .
3. Bartlett, M., 1957: Measles periodicity and
powder based on the use of liquid chromatography tandem mass spectrometry Analytica Chimica Acta, 572, 113–120. http://dx.doi.org/10.1016/j.aca.2006.05.012 PMid:17723467
13. Commission Decision 2003/181/EC, 13 March 2003, amending decision 2002/657/EC as regards the setting of minimum performance limits (MRPLs) for certain residues in food animal origin, Off. J. Eur. Union, L71/17, 2003.
Ge Bai, Hui Li, Yansong Ge, Qianzhen Zhang, Jiantao Zhang, Mingzi Chen, Tao Liu and Hongbin Wang
pig was pre-medicated subcutaneously with 0.05 mg/kg of atropine (Beijing Shuanghe Pharmaceutical, China) and 15 min later intramuscular injections of xylazine (1 mg/kg) and ketamine hydrochloride (10 mg/kg) were given and intravenous administration of propofol (3.5 mg/kg) was used to induce anaesthesia. The animal was intubated with a 6.0–7.0 mm tracheal tube and maintained with 1.5%–3% isoflurane in oxygen after intubation. Laparoscopic hepatic ischaemia-reperfusion and partial resection were performed after establishing a 4-portal approach. After laparoscopic
Alhaji Zubair Jaji, Adamu Saleh Saidu, Mohammed Bakari Mahre, Mbaya Pindar Yawulda, Ibrahim Alhaji Girgiri, Piyush Tomar and Faruk Dau
). Histomorphometric study of the prenatal development of the circumvallate papillae of one-humped camel (Camelus dromedarius). Anat Physiol. 5, 1. https://doi.org/10.5376/ijmz.2015.05.0001
7. Maina, M.M., Usende, I.L., Igwenagu, E., Onyiche, T.E., Yusuf, Z.M., Ntung, N.O. (2014). Gross, histological and histomorphometric studies on the spleen of one humped camel (Camelus dromedarius) found in the semi-arid region of North Eastern Nigeria. J. Vet. Adv. 4 (10): 703-711. https://doi.org/10.5455/jva.20141025045543
8. Jaji, A.Z., Kwari, H.D., Ribadu, A.Y., Sivachelvan, M
Tomasz Grenda, Magdalena Grabczak, Magdalena Goldsztejn, Nina Kozieł, Krzysztof Kwiatek, Krystyna Pohorecka, Marta Skubida and Andrzej Bober
agar (FAA) medium plates. The first of these media comprised peptic digest of animal tissue 10 g/L, meat extract 10 g/L, sodium chloride 5 g/L, lactose 12 g/L, neutral red 0.032 g/L, and agar 10 g/L, with final pH 7.0 ± 0.2 at 25°C and the second peptone 23 g/L, sodium chloride 5 g/L, soluble starch 1 g/L, sodium bicarbonate 0.4 g/L, glucose 1 g/L, sodium pyruvate 1 g/L, L-cysteine HCl×H 2 O 0.5 g/L, sodium pyrophosphate 0.25 g/L, L-arginine 1 g/L, sodium succinate 0.5 g/L, Hemin 0.01 g/L, vitamin K 0.001 g/L, and agar 12 g/L, with final pH 7.2 ±0.2 at 25°C. The