Domingo García-Villamisar and Araceli Del Pozo Armentia
An emerging literature has begun to document the emotional consequences of everyday executive functions on emotional distress. Little is known, however, about whether this relation is mediated by other variables.
A multiple mediation model was proposed to integrate core concepts of daily executive dysfunctions with emotional distress in order to increase understanding of their relationship to psychological well-being and emotional regulation. The working hypothesis was that dimensions of well-being and emotional regulation may be a suitable mediator. It was hypothesized that dimensions of well-being and emotional regulation are a possible mediator between the executive dysfunctions and psychological distress. Participants were 122 undergraduate students from university courses in General Education and Social Education at, the Universidad Complutense de Madrid, with a mean age of 21.76. Results indicate that several dimensions of psychological well-being and emotional reappraisal strategies mediate the relationships between daily executive dysfunctions and psychological distress. Implications of these data are discussed.
Chris Donga, Jun Chen, Xiaoyan Zhang and Congjian Xu
Background: How to deliver sufficient therapeutic genes into cancer cells while causing fewer side effects are major challenges in cancer gene therapies. Receptor mediated gene delivery enables specific delivering therapeutic genes into target cancer cells and increases the efficacy.
Objectives: We developed a novel receptor mediated short interference RNA (siRNA) expression plasmid delivery system against ovarian carcinoma cells with improved biocompatibility in vitro.
Methods: Follice stimulating hormone (FSH) peptide (β: 33-55) was grafted to polyethylenimine (PEI) via a bifunctional polyethylene glycol (PEG) and complexed with anti chemokine c-x-c motif ligand 1 (CXCL1) plasmid DNA (pDNA) to form FSH-PEG-PEI/pDNA polyplexes at various N/P ratios. The cytotocity, transfection efficiency, and CXCL1 silencing effect were evaluated respectively.
Results: The FSH-PEG-PEI/pDNA polyplexes showed low cytotoxicity, high transfection efficiency, and specific CXCL1 gene silencing effect. The 2% FSH-PEG-PEI conjugate complexed with pDNA at N/P ratio of 25 has better physicochemical properties. The therapeutic siRNA expression plasmid uptake by follice stimulating hormone receptor (FSHR) expression ovarian carcinoma cells was through a receptor-mediated manner.
Conclusion: The FSH-PEG-PEI conjugate can be used as a novel receptor mediated gene delivery system against ovarian carcinomas. This strategy could be extended to a wide range of targeted gene therapeutics against different cancers.
Raditsa N. Sokolova, Rumyana K. Yankova, Tsvetana I. Abadjieva, Tatyana A. Popova, Maria V. Ivanovska, Mariana A. Murdjeva, Maria M. Orbetzova, Svetlan M. Dermendzhiev and Maria V. Pavlova
Background: Changes in lifestyle and obesity in recent decades have brought about a dramatic increase in type 2 diabetes mellitus (DM2) and allergic diseases. Clinical and epidemiological studies associate obesity with epidemics of allergic diseases. The link between obesity and DM2 with immunological components of IgE-mediated allergic inflammation is not yet conclusively established.
Aim: To examine the key immunological components of IgE-mediated allergic inflammation in patients with DM2 and their relationship with glycemic control and anthropometric indicators.
Materials and methods: Fifty-five patients with DM2 and 32 healthy controls with normal weight and body mass index (BMI) of 18-24.9 kg/m2 were included in the study. Th2-cytokine profile (serum levels of IL-4 and IL-5, pg/ml) and total serum IgE IU/ml were assessed in all participants in the study using ELISA. In patients with DM2, levels of glycated hemoglobin (HbA1c%) in the blood were also measured.
Results: Serum levels of IL-4 and IL-5 are significantly higher in patients with DM2 compared to the control group. Serum levels of IL-4 and IL-5 positively correlated with BMI as well as serum levels of IL-4 with waist circumference. Total serum IgE positively correlated with HbA1c.
Conclusion: Obesity and poor glycemic control in patients with DM2 affect key immunological components of IgE-mediated allergic inflammation and possibly alter the immune response to allergens and antigens.
Ganna M. Shayakhmetova, Larysa B. Bondarenko, Anatoliy V. Matvienko and Valentina M. Kovalenko
). Induction of CYP3A by ethanol in multiple in vitro and in vivo models. Alcohol Clin Exp Res 27: 981-988.
Fernandez A, Colell A, Caballero F, Matias N, Garcia-Ruiz C, Fernandez-Checa JC. (2009) Mitochondrial S-adenosyl-L-methionine transport is insensitive to alcohol-mediated changes in membrane dynamics. Alcohol Clin Exp Res 33: 1169-1180.
Forkert P-G, Lash LH, Nadeau V, Tardif R, Simmonds A. (2002). Metabolism and toxicity of trichloroethylene in epididymis and testis. Toxicol Appl Pharmacol 182: 244-254.
Frias J, Rodriguez
, Mitre A, Korodi S, et al. Epicardial Fat, Paracrine-mediated Inflammation and Atrial Fibrillation. Journal of Interdisciplinary Medicine . 2017;2:304-307.
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as a spiritless legalism, Joël venerated the sage of Königsberg for leaving him room for religious belief. Since Kant did not deny the existence of noumena, even if they could not be brought to cognition, he left metaphysical space for the existence of God beyond the categories of the human mind. Kant, as Joël saw him, was a mediator between philosophy and religion even as Joël sought to be a mediator between Judaism and European culture. Karl Joël, “Erinnerungen an Manuel Joël,” 318; Isaak Heinemann, Manuel Joels wissenschaftliches Lebenswerk (Breslau, 1927), 14
The influence of Pseudomonas aeruginosa secreted virulence factors on hemocyte-mediated immune response of Galleria mellonella
The influence of secreted virulence factors (SVF) of entomopathogenic strain of Pseudomonas aeruginosa on Galleria mellonella hemocytes-mediated immune response mechanisms was examined. The obtained results indicated that larvae injection with SVF adversely affected the morphology and consequently viability of hemocytes. Moreover, factors presented in culture supernatant lead to aberrant organization of the actin cytoskeleton. Within the time of SVF action, hemocytes preserved only the ability to adhere to the foreign surfaces in comparison to cells collected from naïve larvae, which formed pseudopodia and extensively spread. It seemed probable that cytoskeleton disruption led to overcoming hemocytes-mediated immune response mechanisms by entomopathogen. The phagocytosis in treated with SVF larvae was observed but hemocytes which engulfed bacteria became dead. Moreover, hemocytes ability to capsule formation was strongly inhibited in SVF-treated larvae.
Ramesh K. Saini, Muthu K. Akithadevi, Parvatam Giridhar and Gokare A. Ravishankar
- Isoflavone content in soybean seeds was enhanced by the elicitor-mediated approach under field conditions through the floral application of abiotic elicitors-salicylic acid, methyl jasmonate and biotic elicitors-Aspergillus niger and Rhizopus oligosporus. Among isoflavones, daidzein and glycitein were found to be highly responsive to elicitors, with an increase of 53.7% and 78.7%, respectively as compared to control. Highest total isoflavone content (1276.4 mg g-1 of seeds) was observed upon the administration of 0.1 mMsalicylic acid, which is 92.7% higher than in control. This study would be valuable for augmentation of the isoflavone content in soybean seeds in field grown plants for better nutraceutical potential.
In this study, we examined the impact of emotional intelligence on sales performance. We posited that the impact of emotional intelligence (EI) on sales performance was mediated by adaptive selling behaviour (ASB). Data were collected from 281 sales people in the financial industries in Malaysia via the WLEIS emotional intelligence scale and ADAPTS adaptive selling behaviour scale, and were quantitatively analysed using structural equation modelling (SEM). Results were in keeping with the model. Three domains of EI were not found to impact sales performance directly but through ASB. Theoretical implications and managerial ramifications were also discussed.
Background: Viral vectors can produce longer-lasting effects than a recombinant protein.
Objective: Develop a system of sustained GH1 (a human growth hormone gene) transfer and expression in baby hamster kidney cell (BHK-21) line using recombinant adeno-associated viral vectors pseudotyped with viral capsids from serotype 1 (rAAV2/1).
Methods: The expression of GH1 in vitro was examined by reverse transcription polymerase chain reaction and Western blot analysis. Effects of GH1 on cell proliferation were measured by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide (MTT) experiments. Mice serum IGF-1 and blood glucose, after injection of virus infected BHK-21 cells, were measured by enzyme-linked immunosorbent assays.
Results: rAAV2/1-mediated GH1 gene transfer occurred effectively in vitro after 48 hours of transduction at 1 105 vg/mL (multiplicities of infection). MTT experiments indicated notable effects of the GH1 gene on cell proliferation in vitro. Subsequent animal experiment suggested that the injection of virus infected BHK-21 cells might induce increase of serum IGF-1.
Conclusion: Our study shows the feasibility of rAAV2/1-mediated GH gene delivery in vitro, applicable for future experimental and clinical investigations.