Maja Sereg-Bahar, Ales Jerin and Irena Hocevar-Boltezar
. Quadeer MA, Lopez R, Wood BG. Does acid suppresive therapy reduce the risk of laryngeal cancer recurrence? Laryngoscope 2005; 115: 1877-81.
8. Copper MP, Smit CF, Stanojcic LD, Devriese PP, Schouwenburg PF Mathus- Vliegen LM. High incidence of laryngeal reflux in patients with head and neck cancer. Laryngoscope 2000; 110: 1007-11.
9. Belafsky PC, Postma GN, Koufman JA. Validity and reliability of the reflux Symptom Index (RSI). J Voice 2002; 16: 274-7.
10. Samuels TL, Johnston N. Pepsin as a marker of extraesophageal
Martin Gierus, Birgit Eickler, Reinhard Resch, Ralf Loges, Friedhelm Taube and Erich M. Poetsch
OMD, organic matter digestibility; TT, Tilley and Terry method; CM, pepsin-cellulase method; ME, metabolizable energy; WC, white clover; RC, red clover; LC, lucerne; BT, birdsfoot trefoil; KC, kura clover; RG, rotational grazing; CM-OMD, OMD based on pepsin-cellulase method; TTOMD, OMD based on Tilley and Terry method; ME CM , ME estimated by pepsin-cellulase method; ME TT , ME estimated by Tilley and Terry method; MAT, mean average temperature; AP, average precipitation.
With the genetic advancement of new cultivars
AHyaluronic acid (HA) is part of the extracellular matrix of connective, epithelial and neural tissues, as well as the synovial fluid, skin, and cartilage. It is composed of repeating disaccharide units of D-glucuronic acid and N-acetyl glucosamine. Hyaluronic acid is used in abdominal surgery, ophthalmology, dermatology, rhinology; it is usable for the osteoarthritis treatment. The membranes of eggshell are a natural source of hyaluronic acid, collagen, glycosaminoglycan and collagenous proteins. In paper, we tested the possibility of extraction hyaluronic acid from the eggshell membranes by enzymatic hydrolysis. We identified optimal conditions of hydrolysis with trypsin at reaction temperature of 37 °C and pH 8; with pepsin at 40 °C and pH 3, as well as with papain at 60 °C and pH 7.5. The content of hyaluronic acid in samples was determined spectrophotometrically using the carbazole method. The experimental results showed a yield of ~ 4 -4.5 % hyaluronic acid per 1 g of dry eggshell membranes.
Aleksandra Zambrowicz, Monika Timmer, Ewelina Eckert and Tadeusz Trziszka
Increasing the potency of antihypertensive food-derived peptides is a critical and important step in the development of natural drugs for cardiovascular diseases prevention. We have proposed the egg-white protein precipitate (EWPP) obtained as a byproduct of cystatin and lysozyme isolation as a potential source of ACE-inhibitory peptides derived by pepsin digestion. The results indicated that hydrolysis of EWPP with pepsin produced the ACE inhibitory activity. During 3-h hydrolysis (DH: 38.3%), the IC50 value of EWPP hydrolysate was significantly increased and finally reached IC50=643.1 μg/mL. This hydrolysate was further fractionated by RP-HPLC. The peptide fraction exhibiting the highest ACE inhibitory activity was rechromatographed. Three peptide subfractions exhibiting ACE-inhibitory activities of 69.0, 25.0, and 37.6 μg/mL were further characterised. In each of them, mixtures of peptides with different molecular masses were observed.
K., Tomita M., Giehl T.J., Ellison R.T., Antibacterial activity of lactoferrin and a pepsin-derived lactoferrin peptide fragment. Inf. Immun., 1993, 61, 719-728.
Ye X.Y., Wang H.X., Liu F., Ng T.B., Ribonuclease, cell-free translation-inhibitory and superoxide radical scavenging activities of the iron-binding protein lactoferrin from bovine milk. Int. J. Biochem. Cell. Biol., 2000, 32, 235-241.
Yoo Y.C., Watanabe R., Koike Y., Mitobe M., Shimazaki K., Watanabe S., Azuma I., Apoptosis in human leukemic cells
Agnieszka Kosińska, Anna Urbalewicz, Kamila Penkacik, Magdalena Karamać and Ryszard Amarowicz
SE-HPLC-DAD Analysis of Flaxseed Lignan Macromolecule and its Hydrolysates
A lignan macromolecule (LM) was extracted from defatted flaxseeds using an ethanol-dioxan system (1:1, v/v) and purified using Amberlite column chromatography with water and methanol as mobile phases. The LM was subjected to chemical hydrolysis (base, acid, base & acid), as well as to enzymatic processing using pepsin, pancreatin, cellulase, and β-glucuronidase.
The study revealed that lignan macromolecule in flaxseed was not homogenous. The chemical hydrolysis as well as enzymatic treatment using β-glucuronidase and cellulase released low molecular phenolic compounds from the lignan macromolecule. The liberation of secoisolariciresinol (SECO) and free phenolic acids (p-coumaric and ferulic acids) from flaxseed lignan macromolecule as a result of the base and acid hydrolyses was noted. The application of pepsin and pancreatin did not change the composition of the lignan macromolecule.
Anna Rzepecka-Stojko, Barbara Pilawa, Paweł Ramos and Jerzy Stojko
Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy
Bee pollen is a valuable and highly recognized source of exogenous antioxidants. The aim of these studies was to determine the antioxidant capacity of three types of bee pollen extracts: ethanol extracts of bee pollen, pepsin extracts of bee pollen and ethanol extracts of pepsin-digested bee pollen. Their antioxidant properties were determined with the use of electron paramagnetic resonance (EPR) and their ability to quench DPPH free radicals was estimated. The EPR results showed that ethanol extracts of pepsin-digested bee pollen (EEPP) had the highest antioxidative effect and the highest free radical DPPH scavenging potential. The pepsin extracts of bee pollen (PEP) had the weakest antioxidant capacity. The ability to quench DPPH free radicals was also the weakest one for this extract. An average antioxidative effect was recorded for ethanol extracts of bee pollen (EEP).
Marcel Skejovic Joehnke, Susanne Sørensen, Charlotte Bjergegaard, Keld Ejdrup Markedal and Jens Christian Sørensen
Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose-dependently decreased upon addition of hull fibres at all inclusion levels (8.9-26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3-8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.
Suteera Techatanawat, Rudee Surarit, Theeralaksna Suddhasthira and Siribang-on Piboonniyom Khovidhunkit
Background: Collagen has attracted great interest as a biomaterial for various dental and medical uses.
Objective: Investigate the characteristics and biocompatibility of type I collagen extracted from rat-tail tendon and bovine Achilles tendon for dental application.
Materials and methods: Type-I collagen was extracted from rat-tail and bovine Achilles tendon using pepsin. The purity of collagen extracts was examined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The biocompatibility with human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) was examined using an MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Scanning electron microscope (SEM) illustrations of purified collagen alone and collagen with HGFs and HOKs were presented. A three-dimensional wound-healing model of fibroblast populated collagen lattice (FPCL) was used to determine the capability of both sources of collagen to induce wound healing in vitro. Cellular collagen lattices were fabricated to examine the contraction rate of these collagens.
Results: The average yield of collagen extracted from rat-tail and bovine Achilles tendon were 21.8±14.9% and 5.4±0.4%, respectively. The SDS-PAGE analysis showed that the extracts were composed of alpha 1, alpha 2 and beta chains with little contamination of other small proteins. The MTT assay showed good proliferation of cells cultured with each collagen extract, indicating that collagen extracts were non-toxic to the cells. SEM and the FPCL analysis showed that both types of collagen were biocompatible with both HGFs and HOKs, inducing good contraction in the in vitro model.
Conclusion: Type-I collagen extracted from rat-tail and bovine Achilles tendon appeared to be biocompatible with HGFs and HOKs. Both biomaterials may be of use in dental practice.
Andrzej Noworyta, Anna Trusek and Maciej Wajsprych
application in obtaining peptides. Chem. Eng. J. 305, 61-68. DOI: 10.106/j.cej.2016.05.087.
15. Labus, K., Trusek-Holownia, A. & Noworyta, A. (2015), Kinetics of protein hydrolysis catalyzed by pepsin. In: 42nd International Conference of Slovak Society of Chemical Engineering proceedings, Tatranské Matliare, Slovakia, May 25-29, 2015. Ed. Jozef Markoš, Slovakia, 465-472.