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Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

H., Long B., Wu X., Bai Y., Development of a Loop-Mediated Isothermal Amplification Assay for sensitive and rapid detection of Cronobacter sakazakii. Foodborne Pathog. Dis., 2012, 9, 1111-1118. 11. Francois P., Tangomo M., Hibbs J., Bonetti E.J., Boehme C.C., Notomi T., Perkins M.D., Schrenzel J., Robustness of a loopmediated isothermal amplification reaction for diagnostic applications. FEMS Immunol. Med. Microbiol., 2011, 62, 41-48. 12. Fratamico P.M., Bagi L.K., Cray W.C. Jr., Narang N., Yan X., Medina M., Liu Y., Detection by

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Exogenous isoleucine and phenylalanine interact with abscisic acid-mediated anthocyanin accumulation in grape

. Integr. Genomics 10, 367-381. M ac D onald M.J., D’C unha G.B., 2007. A modern view of phenylalanine ammonia lyase. Biochem. Cell Biol. 85, 273-282. M atus J.T., L oyola R., V ega A., N eira A.P., B ordeu E., J ohnson P.A., et al ., 2009. Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera . J. Exp. Bot. 60, 853-867. M ikami M., M ori D., M asumura Y., A oki Y., S uzuki S., 2017. Electrical stimulation: an abiotic stress generator for

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Does SIRT-1 Mediate Calorie Restriction and Prolong Life? – A Mini Review

Abstract

Calorie restriction is the only intervention proved to prolong both average and maximum lifespan in yeast, worms, fish, rodents and possibly primates. Not only does the regimen prolong life, but it also reduces the incident of numerous age-related diseases like diabetes, atherosclerosis or cancer and slows down ageing. Mechanisms by which that is thought to occur have not yet been elucidated, but they probably involve reactive oxygen species signaling, insulin growth factor and transcriptional factors. Here, special emphasis is given to SIRT1 - silent information regulator. There is sound evidence showing that SIRT1 is a key player in mediating physiological response to calorie restriction and that its overexpression is correlated with extended lifespan. The possible mechanism leading to its elevated levels is high NAD/NADH ratio, observed in Sir2 in yeast. SIRT1 increases glucose production, enhances fat mobilization, stimulates angiogenesis, prevents neuronal degeneration and rises insulin sensitivity. Therefore, it seems to be a very beneficial factor activated by such a simple intervention that is calorie restriction.

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Potential methane production and oxidation along the soil chronosequence of the Rotmoos glacier forefield

C. Deppe M. 2012 Humic acid addition lowers methane release in peats of the Mer Bleue bog, Canada Soil Biology and Biochemistry 52 96 98 Boetius, A., Ravenschlag, K., Schubert, C.J., Rickert, D., Widdel, F., Gieseke, A., Amann, R., Jørgensen, B.B., Witte, U. and O. Pfannkuche (2000): A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 407, 623. 10.1038/35036572 11034209 Boetius A. Ravenschlag K. Schubert C.J. Rickert D. Widdel F. Gieseke A. Amann R. Jørgensen B.B. Witte U

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Phylogenetic analysis of selected representatives of the genus Erica based on the genes encoding the DNA-dependent RNA polymerase I

. & J ansen R. K. 2016. Divergence of RNA polymerase α subunits in angiosperm plastid genomes is mediated by genomic rearrangement. Scientific Reports. DOI: 10.1038/srep24595 Botanical Garden in Kew website. Available online: http://www.kew.org/barcoding/protocols/ (accessed on 21 January 2017). B ritton T., A nderson C. L., J acquet D., L undqvist S. & B remer K. 2007. Estimating divergence times in large phylogenetic trees. Systematic Biology. 56: 741–752. B ruzone M. C., F ontenla S. B. & V ohník M. 2015. Is the prominent ericoid

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Effects of the Invasive Ctenophore Species, Mnemiopsis Leidyi, on the Caspian Sea

ABSTRACT

Human interventions on the Earth’s natural systems are evident even in remote regions of the Antarctic and rain forests deep within the Amazon. In addition to human-induced climate change and habitat destruction, an emerging anthropogenic threat to biodiversity is the drastic species re-distribution (the movement of species from one place to another due to human intervention) at a global scale.

This creates fertile conditions for biological invasions which in turn cause substantial economic and ecological losses. These human-mediated invasions, often referred to as “biological pollution”, are a worldwide problem that is increasing in frequency and magnitude, causing significant damage to the environment, economy and human health. Bioinvasions have strong impact on biodiversity and ecosystem functioning and stability. They are ranked as the second most important threat to biodiversity (after habitat destruction) by the World Conservation Union.

The Ctenophore, Mnemiopsis leidyi, is one of the invasive species that naturally lives in the Atlantic coastal waters of North America and South America, but discovered in Azov, Black, Caspian, North, Baltic and Mediterranean (north-eastern part) seas in early 1980s.

It seems that the main factor of its redistribution was the ballast waters of ships. As an alien species, Mnemiopsis leidyi caused many alterations in the Caspian Sea ecosystems. The fact that it feeds on the eggs of native fish Clopeonella spp., has resulted in a significant decline of its population; Clopeonella spp. were the main source of industrial fishing in the Caspian Sea and also the main source of food for precious fish species, the sturgeons, and therefore, their decline has caused a huge economical loss for the area's inhabitants and a significant decline of sturgeon populations.

This species has caused massive ecosystem changes and substantial economic losses in the late 1980s-1990s, and it has been recognized as a problem of main ecological concern for the sustainable development of the region, together with the high level of anthropogenic pressures on the Caspian Sea ecosystems. Some special characteristics of this species, such as adaptation to a wide range of salinity and temperature, high capability of reproduction, hermaphroditism and dissogeny, have led to huge increases of its mass, especially in southern regions of the Caspian Sea, the coastal waters of Iran. In addition, it has become clear that this species does not have any natural predators in the Caspian Sea, and also that it can feed on any organisms smaller than itself in size. Owing to these facts, it is a huge ecological threat for the Caspian Sea ecosystems. The aim of the present paper is to review the biological and ecological impacts of this invasive species on the Caspian Sea ecosystems.

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Change in the Parameters of Soils Contaminated by Oil and Oil Products

REFERENCES AGAMUTHU, P. ‒ TAN, Y.S. ‒ FAUZIAH, S.H. 2013. Bioremediation of hydrocarbon contaminated soil using selected organic wastes. In Procedia Environmental Sciences , vol. 18 , pp. 694 – 702. DOI: org/10.1016/j.proenv.2013.04.094 AGNELLO, A.C. – BAGARD, M. – HULLEBUSCH, E.D. – ESPOSITO, G. – HUGUENOT, D. 2016. Comparative bioremediation of heavy metals and petroleum hydrocarbons co-contaminated soil by natural attenuation, phytore-mediation, bioaugmentation and bioaugmentation-assisted phytoremediation. In Science of the Total Environment

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Growth of Potato Shoot Cultures on Media with Antibiotics for Elimination of Bacterial Contamination

. 3987 – 3994. MURASHIGE, T. – SKOOG, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. In Physiologia Plantarum , vol. 15 , pp. 473 – 497. DOI: 10.1111/j.1399-3054.1962.tb08052.x OGAWA, Y. – MII, M. 2005. Evaluation of 12 b-lactam antibiotics for Agrobacterium-mediated transformation through in planta antibacterial activities and phytotoxicities. In Plant Cell Reports , vol. 23, no. 10 – 11, pp. 736 – 743. DOI: 10.1007/s00299-004-0885-9 ORLIKOWSKA, T. – NOWAK, K. – REED, B. 2017. Bacteria in the plant tissue

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The Nightmare: Genetically Modified Organisms as Alien Species

Microbial Technology, 10, 54, 38-44. 12. Hammond B., Kough J., Herouet-Guicheney C. and Jez J. M., 2013 ‒ Toxicological evaluation of proteins introduced into food crops, Critical Reviews in Toxicology, 43, 2, 25-42. 13. Hüsken A. and Dietz-Pfeilstetter A., 2007 ‒ Pollen-mediated intraspecific gene flow from herbicide resistant oilseed rape (Brassica napus L.), Transgenic Research, 16, 5, 557-69. 14. Ihemere U., Arias-Garzon D., Lawrence S. and Sayre R. 2006 ‒ Genetic modification of cassava for enhanced starch production

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Responses of Two Barley Subspecies to In vitro-Induced Heavy Metal Stress: Seeds Germination, Seedlings Growth and Cytotoxicity Assay

. – REHMAN, A. – CHEEMA, S.A. – SHARMA, A. – SONG, H. – REHMAN, S. – ZHAORONG, D. 2018. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. In Ecotoxicology and Environmental Safety , vol. 147 , pp. 935 – 944. SINGH, R. – UPADHYAY, A.K. – SINGH, D.P. 2018. Regulation of oxidative stress and mineral nutrient status by selenium in arsenic treated crop plant Oryza sativa. In Ecotoxicology and Environmental Safety , vol. 148 , pp. 105–113. STREET, R.A. – KULKARNI, M.G. – STIRK, W.A. – SOUTHWAY, C

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