Jiangeng Han, Xin Wang, Weiyi Chai, Kunbin Liu and Chen Wang
substantially less than that in Group C, having a statistically significant difference ( P < 0.05; see Table 2 ).
Between-Group Comparison of the Prevalence of Invasive Fungal Infection (cases, %)
Number of Cases
Number of Cases
Group A vs Group B
χ 2 value
Group A vs Group C
χ 2 value
Group B vs
Hooper L.V. Gordon J.I. Commensal host-bacterial relationships in the gut Science 2001 292 1115 – 8
 Qin J., Li R., Raes J., Arumugam M., Burgdorf K.S., Manichanh C., et al., A human gut microbial gene catalogue established by metagenomic sequencing, Nature, 2010, 464, 59-65 10.1038/nature08821 20203603 Qin J. Li R. Raes J. Arumugam M. Burgdorf K.S. Manichanh C. et al A human gut microbial gene catalogue established by metagenomic sequencing Nature 2010 464 59 – 65
 Larsson E., Tremaroli V., Lee Y.S., Koren
You-wen Tan, Jian-cheng Wu, Yun Ye, Li Chen and Peng-li Pai
1. Xie G, Wang X, Wang L, Atkinson RD, Kanel GC. Role of differentiation of liver sinusoidal endothelial cells in progression and regression of hepatic fibrosis in rats. Gastroenterology 2012;142(4):918-927.
2. Tomiyama C, Watanabe H, Izutsu Y, Watanabe M, Abo T. Suppressive role of hepatic dendritic cells in concanavalin A-induced hepatitis. Clin Exp Immunol 2011;166(2):258-268.
3. Kimura K, Ando K, Ohnishi H, Ishikawa T, Kakumu S, Takemura M, et al. Immunopathogenesis of hepatic fibrosis in chronic
Mingxing Su, Congsong Sun, Haiying Wang, Chunyu Yuan, Ruixia Guo, Yajie Liang, Chao Liu and Qiang Wang
different concentrations of ATO on RBC count. B : Effect of different concentrations of ATO on HGB content. C : Effect of different concentrations of ATO on RDW. ATO, arsenic trioxide; RBC, red blood cell; HGB, hemoglobin; RDW, red blood cell distribution width; CON, control group; LD, low-dose group; MD, medium-dose group; HD, high-dose group. * P < 0.05, ** P < 0.01, as compared with CON.
Dynamic effects of ATO exposure on rat platelet parameters
Figure 2 shows that there were significant reductions in platelet (PLT) counts in the HD group 6 h after
Wang Zhen-fei, Mu Yong-ping, Liang Jun-qing, Liu Yong-yan and Li Jing-quan
/vol) solution of low-melting-point agar was prepared in normal medium, placed in six-well culture plates, and allowed to solidify. Cells were suspended in RPMI-1640 medium containing 0.3% low-melting-point agar (10 4 /0.5 ml), and the extract was added into the suspension. Then, the suspension was covered on the lower agar layer. The cells were incubated at 37°C in a humidified atmosphere containing 5% CO 2 for 3 weeks. Colonies of sizes >100 μm were counted under a microscope.
Determination of invasion capacity
Matrigel was diluted with a precooled serum
SK-MES-1 cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 0.1% penicillin-and-streptomycin (complete RPMI-1640 medium) at 37°C in a humidified atmosphere containing 5% CO 2 . The cells in logarithmic growth phase were used.
The viability of 16 HBE cells was measured using CCK-8 analysis. The 16 HBE cells were suspended in complete RPMI-1640 medium at a concentration of 2.5 ×10 4 cells/ml, and 0.2 ml of the suspension was added into each well of 96-well plates. After 12 hour culture, the cells were treated with different
Hepatitis, Geneva, 63rd World Health Assembly, 2000-01-05 [2013-01-16], http://www.docin.com/p-289449237.html . WHO Report from the Secretariat on the Viral Hepatitis, Geneva, 63rd World Health Assembly 2000-01-05 [2013-01-16] http://www.docin.com/p-289449237.html
 Hsia C.C., Purcell R.H., Farshid M., Lachenbruch P.A., Yu M.Y., Quantification of hepatitis B virus genomes and infectivity in human serum samples, Transfusion, 2006, 46(10), 1829-1835. 10.1111/j.1537-2995.2006.00974.x Hsia C.C. Purcell R.H. Farshid M. Lachenbruch P.A. Yu M.Y. Quantification of
Chao Meng, Na Li, Zhaoxiao Tong, Huangxin Yan and Shenxiao Min
. Immunity, 2010, 33(4):542-54.
5 Lawn SD, Harries AD, Anglaret X, et al . Early mortality among adults accessing antiretroviral treatment programmes in sub-Saharan Africa. AIDS, 2008, 22(15):1897-908.
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8 World Health Organization. Global tuberculosis control: WHO Report 2010[EB/OL]. (2010-07-31)[2011-05
nephelometer was 0.5 MacFarland; then the bacterial suspension was diluted with M-H broth to 1.5 × 10 5 CFU/mL stock.
Preparation of Antimicrobial Stock Solution
We referred to the specifications provided by the National Standard Substance Center, aseptic distilled water and 0.1 mol/L NaOH were used as the solvents to dissolve ceftriaxone and levofloxacin to the final concentration of 1280 μg/mL. We used anhydrous ethanol as solvent to dissolve allicin to the final concentration of 4096 μg/mL.
Determination of MIC of Allicin Separately
The MIC of the
Lei Gu, Wen Wen, ZhiXian Wu, Kai Bai, Wei Liu, GuoXiang Lai and DongLiang Li
automated hematology analyzer and its matching reagents (Kobe, Japan). The normal reference value of PLT is (100–300) × 10 9 /L, and the abnormal value of PLT is >300 × 10 9 /L (high PLT group) or <100 × 10 9 /L (low PLT group). Patients were divided into two groups: a normal PLT group and an abnormal PLT group (including low PLT group and high PLT group).
All statistical analyses were performed with using SPSS 17.0 statistical software (SPSS, Chicago, IL, USA), and P -values < 0.05 were defined as statistically significant. The association