Hai-Feng Liu, Hui Li, Ge Bai, Qian-Zhen Zhang, Xiang-Gao, Tao Liu and Hong-Bin Wang
(WBC) and red blood cell (RBC) counts and was performed immediately after sample collection with an MEK-7222K haematology analyser (Nihon Kohden Corporation, Japan). Serum was stored at −80°C after centrifugation.
A total of 300 mg of xylocaine was injected intravenously for euthanasia after general anaesthesia with xylazine and ketamine hydrochloride at 13 weeks, or earlier if a serious complication occurred.
Histopathological examination. Tissue was collected from the caudal pole of the remnant kidney. The renal tissue was fixed in 10% buffered formalin
Agnieszka Troszok, Ludmiła Kolek, Joanna Szczygieł, Tomasz Ostrowski, Mikołaj Adamek and Ilgiz Irnazarow
−80°C until use. The tissue culture infective dose (TCID 50 /ml) for CCB cells was estimated by the Spearman–Kärber method ( 17 ) and was 5.21 × 10 5 TCID 50 /ml.
Infection and experimental set-up . CCB and KF1 cells were seeded at 2 × 10 5 cells/cm 2 on six-well plates (Orange Scientific, Belgium). After 24 h incubation, the cells were infected with CyHV-3 virus at 216 PFU/well for 2 h, then the medium was replaced with 2 ml of the culture medium supplemented with 2% DMSO alone (controls) or containing 66.67 μM of T-ACV. The control for calculations
1. Centre for Science and Environment, 2014: Antibiotics in Chicken Meat: study . New Delhi: Pollution Monitoring Laboratory, 36 p. PML/PR-48/2014.
2. Cháfer-Pericás, C., Maquieira, Á., Puchades, R., 2010: Fast screening methods to detect antibiotic residues in food samples. Trends in Analytical chemistry , 29, 9, 1038—1049. DOI:10.1016/j.trac.2010.06.004.
3. European Commission, 2002: Commission Decision 2002/657/EC of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the
May Phonvisay, Jai-Wei Lee, Jhong-Jie Liou, Hsian-Yu Wang and Chun-Yen Chu
stimulated with RA antigen. Total RNA was extracted, RT-PCR performed, and relative quantitation carried out using GAPDH as the reference gene, where fold change = [(E target ) Χ (control CP target − treatment CP target )] / [(E ref ) Χ (control CP ref − treatment CP ref )].
Cross-detection of antibodies . Cross-reactivity of antibodies to heterologous serotypes was examined by Western blot analysis. Different serotypes of RA, including serotypes 1, 2, 6, and RA56a (of unknown serotype), were cultured, denatured (90°C for 10 min), and loaded onto a 10% SDS
J. Novotný, P. Reichel, K. Bárdová, P. Kyzeková and V. Almášiová
1. Alexopoulos, C., Papaioannou, D. S., Fortomaris, P., Kyriakis, C. S., Tserveni-Goussi, A., Yannakopoulos, A., Kyriakis, S. C., 2007: Experimental study on the effect of in-feed administration of a clinoptilolite-rich tuff on certain biochemical and haematological parameters of growing and fattening pigs. Livest. Sci. , 111, 230—241. DOI: 10.1016/j.livsci.2007.01.152.
2. Ball, R. O., Aherne, F. X., 1982: Effect of diet complexity and feed restriction on the incidence and severity of diarrhoea in early-weaned pigs. Can. J. Anim. Sci
Kai K. Komine Y. Komine K. Asai K. Kuroishi T. Kozutsumi T. Itagaki M. Ohta M. Kumagai K. Effects of bovine lactoferrin by the intramammary infusion in cows with staphylococcal mastitis during the early non-lactating period J Vet Med Sci 2002 64 873 878
9 Lasagno M., Ortiz M., Vissio C., Yaciuk R., Bonetto C., Pellegrino M., Bogni C., Odierno L., Raspanti C.: Pathogenesis and inflammatory response in experimental caprine mastitis due to Staphylococcus chromogenes Microb Pathog 2018, 116, 146–152. 10.1016/j
Ewelina Czyżewska-Dors, Małgorzata Pomorska-Mól, Arkadiusz Dors, Aneta Pluta, Katarzyna Podgórska, Krzysztof Kwit, Ewelina Stasiak and Anna Łukomska
( 13 , 24 ).
BALF collection and total cell counts . Bronchoalveolar lavage fluid was obtained from each animal post mortem . A tracheal tube was inserted into an incision made in the trachea, and each lung was lavaged with 10 ml of PBS, yielding approximately 5 mL of recovery. Cells were collected from BALF by centrifugation at 500 × g for 10 min at 4°C. The concentration of nucleated cells in BALF was determined by counting in Türk’s solution, and approximately 3 × 10 6 BALF cells were prepared for cytokine gene expression evaluation. The supernatant was
Sample collection and analysis . Blood samples of each cow were collected daily before morning feeding by venepuncture of the caudal vein, and heparin sodium was used as an anticoagulant. The samples were immediately centrifuged at 1,400 g for 10 min. The plasma was subsequently stored at −80°C. Blood parameters, including BHBA, nonesterified fatty acids (NEFA), glucose (Glu), calcium (Ca), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triglycerides (TG), total bilirubin (TBIL), and γ-glutamyl transpeptidase (GGT) were
25.79 ± 2.42 b
29.46 ± 0.82 b
42.27 ± 1.16 a
42.39 ± 2.46 a
GST (U/mg protein)
23.19 ± 0.84 a
21.05 ± 0.80 ab
14.96 ± 0.84 c
13.87 ± 1.02 c
21.03 ± 0.90 ab
19.97 ± 1.19 b
SOD (U/g protein)
77.49 ± 0.25 a
77.19 ± 0.31 ab
74.18 ± 0.22 c
73.00 ± 0.58 c
76.10 ± 0.27 ab
75.83 ± 0.81 b
The data are expressed as mean ± SE for seven animals per group. Within rows, means with different letters (a, b, and c) are significantly different (p < 0.001)
Plasma AST, ALT, ALP, and LDH activities and cholesterol levels