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  • Author: Zbigniew Sieradzki x
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Open access

Tomasz Grenda, Elżbieta Kukier, Zbigniew Sieradzki, Magdalena Goldsztejn and Krzysztof Kwiatek

Abstract

The aim of this study was to perform an in-house validation of multiplex PCR method for C. botulinum detection in food and feed samples. The study was carried out on food and feed matrixes artificially contaminated by spores of C. botulinum reference strains. The following characteristic parameters for qualitative detection were estimated: limit of detection expressed as LOD50 according to the Spearman-Kärber formula, specificity, sensitivity, and accuracy according to the PN-EN ISO 16140:2004. The validated method showed high specificity. Specific PCR products were revealed only for DNA obtained from samples contaminated with C. botulinum spores. PCR inhibition was observed, especially during examination of contaminated feed. The calculated LOD50 for feed was nearly 10 times higher than for food. The implemented method enables to obtain test results during 3 d without time- consuming process of isolation and proving the ability of strains to produce botulinum toxins.

Open access

Z. Sieradzki, M. Mazur, K. Kwiatek, S. Świątkiewicz, M. Świątkiewicz, J. Koreleski, E. Hanczakowska, A. Arczewska-Włosek and M. Goldsztejn

Abstract

The aim of this study was to assess the possibility of genetically modified DNA transfer from feed containing RR soybean or/and MON810 maize to animal tissues, gut bacterial flora, food of animal origin, and the fate of GM DNA in the animal digestive tract. The experiment was carried out on broilers, laying hens, pigs and calves. All animals were divided into four groups: I - control group (non-modified feed), II - GM soybean group (non-modified maize, RR soybean), III - GM maize group (MON810 maize, non-modified soybean), and IV - GM maize and soybean group (MON810 maize, RR soybean). Samples of blood, organs, tissues, digesta from the gastrointestinal tract, and eggs were analysed for the presence of plant species specific genes, and transgenic sequences of CaMV 35S promoter and NOS terminator. PCR amplifications of these GM sequences were conducted to investigate the GM DNA transfer from feed to animal tissues and bacterial gut flora. In none of the analysed samples of blood, organs, tissues, eggs, excreta and bacterial DNA were plant reference genes or GM DNA found. A GM crop diet did not affect bacterial gut flora as regards diversity of bacteria species, quantity of particular bacteria species in the animal gut, or incorporation of transgenic DNA to the bacteria genome. It can be concluded that MON810 maize and RR soybean used for animal feeding are substantially equivalent to their conventional counterparts. Genetically modified DNA from MON810 maize and RR soybean is digested in the same way as plant DNA, with no probability of its transfer to animal tissues or gut bacterial flora.

Open access

Beata Szymczyk, Witold Szczurek, Sylwester Świątkiewicz, Krzysztof Kwiatek, Zbigniew Sieradzki, Małgorzata Mazur, Dariusz Bednarek and Michał Reichert

Abstract

Introduction

The influence of feeding genetically modified MON 810 hybrid maize on the growth and haematological and biochemical indices of rats was tested.

Material and Methods

Two conventional (non-GM) and two test (MON 810) lines of maize were used in semi-purified diets at the level of 40% w/w. The non-GM I, MON 810 I, non-GM II, and MON 810 II maize lines were near-isogenic. A total of 40 male 6-week-old Wistar-derived rats were assigned to four equal feeding groups corresponding to the four maize lines for 16 weeks. Overall, health, body weight gain, clinical pathology parameters, gross changes, and appearance of tissues were compared between groups.

Results

There were no statistically significant differences in the weight gain or relative organ weights of rats, but there were some non diet-related histopathological changes in the liver, kidneys, and spleen. Except for creatinine level, no diet-related effects were observed in haematology or most of the biochemical indices. Transgenic DNA of MON 810 maize was not detected in the tissues or faeces nor in the DNA of E. coli isolated from the rectum digesta of rats given transgenic feeds. In our experiment, various metabolic indices of rats fed non-GM diets or genetically modified (MON 810) maize for 16 weeks were similar. No adverse nutrition-related health effects were detected.

Conclusion

MON 810 maize seems to be as safe as the conventional maize lines.