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Masoumeh Safaei, Masoud Sheidai, Behnaz Alijanpoor and Zahra Noormohammadi

Abstract

Thirty-nine plant specimens of six Salvia species were collected from different localities of the Alborz mountain region in Iran and studied for morphological and genetic variability and species relationship. Inter simple sequence repeats (ISSR) molecular markers showed a high degree of within-species and interspecific genetic variability in Salvia. Analysis of molecular variance and Hickory tests showed significant molecular difference among the studied populations. A principal coordinate analysis plot of morphological characters grouped the species into two distinct groups, supporting their taxonomic treatment. This was partly supported by ISSR networking. The Mantel test did not show a correlation between genetic distance and the geographical distance of the studied populations. STRUCTURE and reticulation analyses revealed some degree of gene fl ow among the species. The present study showed that ISSR molecular markers could be used in Salvia species delimitation along with morphological study.

Open access

Narjes Azizi, Masoud Sheidai, Valiollah Mozaffarian, Mitra Arman and Zahra Noormohammadi

Abstract

This study conducted to determine relationship among and within Iranian Helichrysum species (Asteraceae). In this study based on ISSR markers, the highest percentage of ISSR loci polymorphism (54.7%) occurred in H. armenium. The highest gene diversity over loci (1.224), Shannon’s Information Index (0.224%) and Expected Heterozygosity (0.142%) occurred in H. armenium (0.18) and the lowest of these parameters (0%) were observed in H. araxinum, H. graveolens, H. persicum and H. psychrophilum. The highest genetic similarity occurred between H. armenium and H. rubicundum (0.989), while the lowest was between H. polyphyllum and H. graveolens (0.213). The analysis of molecular variance (AMOVA), showed significant genetic variation among (24%) and within (76%) species. In morphological analysis traits such as indumentum, resting bud, achene length, achenial papillae, dimension of receptacle and form and apex of phyllaries were main diagnostic features. Results obtained from the morphological cluster were greatly consistent with the molecular data, to elucidating taxonomic relationships, as well as both attributed the higher diversity in H. armenium and H. rubicundum in comparison with other species and also indicated that H. persicum is a member of H. oocephalum species. Totally we confirmed the presence of 18 species in Iran.

Open access

Masoud Sheidai, Melica Tabasi, Mohammad-Reza Mehrabian, Fahimeh Koohdar, Somayeh Ghasemzadeh-Baraki and Zahra Noormohammadi

Abstract

The genus Crocus L. (Iridaceae) is monophyletic and contains about 100 species throughout the world. Crocus species have horticultural, medicinal and pharmacological importance. Saffron is the dried styles of C. sativus and is one of the world’s most expensive spices by weight. Controversy exits about the taxonomy of the genus and the species relationship. Exploring genetic diversity and inter-specific cross-ability are important tasks for conservation of wild taxa and for breeding of cultivated C. sativus. The present study was performed to study genetic variability and population structure in five Crocus L. species including Crocus almehensis Brickell & Mathew, C. caspius Fischer & Meyer, C. speciosus Marschall von Biberstein, C. haussknechtii Boissier, and C. sativus L. by inter simple sequence repeat (ISSR) molecular markers. We also used published internal transcribed spacer (ITS) sequences to study species relationship and compare the results with ISSR data. The results revealed a high degree of genetic variability both within and among the studied species. Neighbor joining (NJ) tree and network analysis revealed that ISSR markers are useful in Crocus species delimitation. Population fragmentation occurred in C. caspius and C. sativus. Both ISSR and sequenced based analyses separated C. sativus from the other studied species. Close genetic affinity of C. sativus and C. pallisii and inter-specific gene flow was supported by both data sets.

Open access

Somayeh Esfandani-Bozchaloyi, Masoud Sheidai, Maryam Keshavarzi and Zahra Noormohammadi

Abstract

Species identification is fundamentally important within the fields of biology, biogeography, ecology and conservation. The genus Geranium L. (Geraniaceae) comprises about 430 species distributed throughout most parts of the world. According to the most recent treatments, subg. Geranium is the largest subgenus with over 370 species classified in ten sections. The subg. Geranium is represented in Iran by 13 species. These species are grouped 3 sections. In spite vast distribution of many Geranium species that grow in Iran, there are not any available report on their genetic diversity, mode of divergence and patterns of dispersal.

Therefore, we performed molecular (ISSR markers) and morphological studies of 102 accessions from 13 species of Geranium (subg. Geranium) that were collected from different habitats in Iran. The aims of present study are: 1) can ISSR markers identify Geranium species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that combination of morphological and ISSR data can identify the species.