Objective: To systematically review the effectiveness of Balance Acupuncture for the Pain in Neck, Shoulder, Low-back and Legs (PNSLL).
Methods: Web-based search on databases such as PubMed（2008.1.～2014.11）、Web of Science（2008.1.～ 2014.11）、Wan Fang Data（2008.1.～2014.11）、CNKI（2008.1.～2014.11）to collect literature of randomized controlled trials (RCT) on Balance Acupuncture for PNSLL. Literature selection, data extraction and methodological quality assessment of the included studies were conducted by two reviewers independently. The meta-analysis was performed by using Rev Man 5.2 software.
Results: A total of 6 studies involving 816 participants were included. Meta-analysis showed that Balance Acupuncture treatment of the PNSLL is superior to traditional Acupuncture, with significant differences (OR=0.12, 95%CI=[0.08,0.15], Z=5.83, P<0.00001).
Conclusion: Balance Acupuncture is an effective and safe way for treating PNSLL, though it may need further demonstration by more large-scale and well-designed RCT due to the limited quantity and quality of the included studies.
A 50-year-old man with ankylosing spondylitis was treated successfully with infliximab, who was also a HBV carrier for about twenty five years. After injection with infliximab for four times, he developed jaundice and HBV DNA was detectable in serum. Serum aminotransferase and total bilirubin levels were higher than normal. Then he was hospitalized and treated with entacavir and Chinese herb medicine. But his liver damage aggravated and was diagnosed as acute on chronic liver failure. Finally, liver transplantation was carried out and he was cured successfully.
The prevalence and microbial pattern reported for Community-acquired pneumonia (CAP) differ considerably and contemporary situation remains changing over time. We therefore searched both international and domestic databases for relevant references and pooled incidence of CAP and etiological distribution were estimated separately between children and adults groups. The results showed that CAP remained a major public health issue in China, with a relatively higher incidence than that reported in Western countries. Although pathogens were not detected in nearly half of patients, Mycoplasma pneumoniae remained to be the most frequently detected agent across age groups, the detection yield of which was lower than that reported from other countries. Notably, the incidence of influenza virus A in adults was almost four times higher than that in children while the prevalence of respiratory syncytial virus was much less common in adults than that in children. Despite some limitations, the value of this review, approaching to systematically review grey published data, is to sketch out the contemporary epidemiological and etiological situation of CAP in our country, which could be useful to help policymakers and clinicians make informed choices and to inspire future studies and surveillance.
Background: The alternative splicing of Bcl-x generates the proapoptotic Bcl-xs protein and the antiapoptotic variant Bcl-xl. Previous studies have demonstrated that some chemotherapeutic agents such as emetine, staurosporine, and epigallocatechin gallate (EGCG) in combination with ibuprofen significantly altered the ratio of the Bcl-x variants Bcl-xs/Bcl-xl in various cell lines, suggesting Bcl-x splicing might be affected by the exogenous stimuli.
Objective: We investigated the regulative role of imatinib in the alternative pre-mRNA splicing of Bcl-x in K562 cells and the related mechanism.
Methods: Cell proliferation was measured using WST assay kit. Cell apoptosis was assayed using an Annexin V-FITC Apoptosis Detection Kit. RT-PCR and western blot assay was used to analyze the mRNA and protein level of alternative splicing of exon 2 in the Bcl-x gene respectively.
Results: Imatinib regulated the alternative splicing in the Bcl-x gene in the K562 cells. In addition, we found that hydroxyurea, another agent for the therapy of CML, could enhance the effect of imatinib on the ratio of the Bcl-xl/Bcl-xs. Moreover, the induction of alternative splicing was correlated with protein phosphatase 1 (PP1). Alternatively, pretreatment with calyculin efficiently blocked imatinib-induced alternative splcing in the K562 cells compared with okadaic acid, which showed an important role of PP1 in regulating imatinib-induced splicing.
Conclusion: Imatinib regulates the alternative splicing of Bcl-x in K562 cells, which may be associated with the activation of PP1.
Objective To identify the risk factors for imipenem resistance development and transmission of clinical Pseudomonas aeruginosa isolates.
Methods Thirty-seven imipenem unsusceptible Pseudomonas aeruginosa isolates collected from patients in absence of carbapenem treatment were characterized by antimicrobial susceptibility test, pulsed field gel electrophoresis (PFGE) and carbapenem resistant mechanism analysis.
Results Before the collection of imipenem unsusceptible Pseudomonas aeruginosa isolates, the average time of patients treated with more than one antimicrobial (20.0 ± 9.5 days, n = 16) was significantly longer than those treated with only one antimicrobial (12.6 ± 4.4 days, n = 21; t-test, Welch, t = -2.9004, P < 0.01). And 32 isolates showed resistance to more than 3 classes of antimicrobials. Six PFGE clusters were identified and 26 isolates were grouped into one dominant cluster (C2). An ISpa1328 sequence insertion in oprD was detected in 33 isolates and the function of efflux was observed in all 37 isolates in the presence of a wide spectrum efflux inhibitor.
Conclusions Our data demonstrated that exposure to non-carbapenem drug classes, especially fluoroquinolones and β-lactams, may be important risk factors for the spread of carbapenem resistant Pseudomonas aeruginosa.
To justify the function of miRNAs in reproductive regulation in swine, the expression of miR-145, miR-429 and their related genes were studied in reproductive tissues of sows. Wannan black pig and Yorkshire pigs with extremely high (n=6) and low (n=6) litter size were sampled, and real-time quantitative PCR (qPCR) was performed on tissue samples from ovaries, uterus, oviduct, hypothalamus, and pituitary. The results indicated that miR-145, miR-429, and zinc finger E-box binding homeobox 1 gene (ZEB1) were expressed significantly different in Wannan black pig and Yorkshire pigs. In pigs with different fecundity, miR-145 in the uterus was expressed significantly lower in pigs with high litter size, than in pigs with low litter size. The miR-429 expression in the oviduct and pituitary of pigs with high litter size was significantly higher compared with tissues sampled from pigs with low litter size. The ZEB1 expression in the pituitary was lower in pigs with high litter size in comparison to pigs with low litter size, while luteinizing hormone beta subunit (LHβ) showed the opposite pattern of expression. In conclusion, miR-145 and miR-429 were differently expressed in pigs with high and low litter size and might have a role in affecting litter size of sows.
Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information.
Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay.
Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results.
Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.