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  • Author: Yun Wei x
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Open access

Yun-Wei Zhang, Yun-Zhuan Zhou, Hai-Bo Lu, Deng-Yu Zheng and Yan-Hua Huang

Abstract

Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.

Open access

Wei Song, Huai-yuan Liu, Bin Xiang, Hong Hu, Cheng-jiang Wang and Ling-yun Wan

Open access

Yun Wei, Ying Yu, Lifeng Xu, Wei Huang, Jianhua Guo, Ying Wan and Jinde Cao

Abstract

Vehicle emission calculation is critical for evaluating motor vehicle related environmental protection policies. Currently, many studies calculate vehicle emissions from integrating the microscopic traffic simulation model and the vehicle emission model. However, conventionally vehicle emission models are presented as a stand-alone software, requiring a laborious processing of the simulated second-by-second vehicle activity data. This is inefficient, in particular, when multiple runs of vehicle emission calculations are needed. Therefore, an integrated vehicle emission computation system is proposed around a microscopic traffic simulation model. In doing so, the relational database technique is used to store the simulated traffic activity data, and these data are used in emission computation through a built-in emission computation module developed based on the IVE model. In order to ensure the validity of the simulated vehicle activity data, the simulation model is calibrated using the genetic algorithm. The proposed system was implemented for a central urban region of Nanjing city. Hourly vehicle emissions of three types of vehicles were computed using the proposed system for the afternoon peak period, and the results were compared with those computed directly from the IVE software with a trivial difference in the results from the proposed system and the IVE software, indicating the validity of the proposed system. In addition, it was found for the study region that passenger cars are critical for controlling CO, buses are critical for controlling CO and VOC, and trucks are critical for controlling NOx and CO2. Future work is to test the proposed system in more traffic management and control strategies, and more vehicle emission models are to be incorporated in the system.

Open access

Wei Guo, Jie Zhang, Jing-yun Li, Yue Ma and Sheng-hui Cui

Abstract

Objective A prospective study was conducted in a tertiary care center to identify the risk factors of ventilator associated pneumonia (VAP) through phenotypic and molecular biological methods.

Methods The patients who were mechanically ventilated in the respiratory intensive care unit (RICU) and the neurological internal intensive care unit (NICU) were enrolled in our study, and samples were collected from the lower respiratory tract, oropharynx and stomach. Other samples, including the environmental air, swabs of nurses’ hands, subglottic secretion and ventilator circuit, were also collected. Microorganisms in the collected samples were recovered and identified at species level by biochemical detection. Genetic relationship of dominant species was further characterized by pulsed field gel electrophoresis (PFGE).

Results Out of 48 enrolled patients, 22 cases developed VAP and bacterial cultures were recovered from the lower respiratory tract samples of 14 cases. The average hospitalization time with VAP was significantly longer than that of patients without VAP (P < 0.05). Among the recovered bacteria cultures, multidrug-resistant Pseudomonas aeruginosa and Stenotrophomonas maltophilia were dominant. It was more likely that subglottic secretion and gastric juice samples contained the same isolates as recovered in the lower respiratory tract by PFGE analysis.

Conclusions Mechanical ventilation in RICU and NICU was a high risk factor for VAP development. Special emphasis of VAP prophylaxis should be paid on subglottic secretion and gastric juice reflux.

Open access

Yun Gao, Mohammad Reza Farahani and Wei Gao

Abstract

In this article, we propose an ontology learning algorithm for ontology similarity measure and ontology mapping in view of distance function learning techniques. Using the distance computation formulation, all the pairs of ontology vertices are mapped into real numbers which express the distance of their corresponding vectors. The more distance between two vertices, the smaller similarity between their corresponding concepts. The stabilities of our learning algorithm are defined and several bounds are yielded via stability assumptions. The simulation experimental conclusions show that the new proposed ontology algorithm has high efficiency and accuracy in ontology similarity measure and ontology mapping in certain engineering applications.

Open access

Yi-Fan Tang, Xin-Yu Hu, Jing Zhao, Ze-Juan Gu, Shuang-Shuang Xing and Wei-Yun Wang

Abstract

Objective

Venous leg ulceration (VLU) is one of the complications of lower extremity venous reflux and reflux disorder of severe diseases, with many adverse effects on patient’s work and life. Nowadays, more and more patients with VLU accept wound care in community setting. Clinical nurses generally take care of the patients based on their own experiences. Healing in VLU is an incredibly complex process, which puzzles even experts. The majority of general nurses do not have this level of expertise, especially those nurses serving in community. Function is the basis of nursing activities. Patients always show different clinical manifestations and self-care abilities due to various function states, which cannot be reflected completely by the existing nursing practice. How to describe nursing practice standardized in order to demonstrate the effectiveness of interventions and facilitate interdisciplinary communication is another urgent problem. Therefore, the aim of this project is to develop an accurate nursing program based on function in chronic venous leg ulcers, which can both satisfy the needs of patients and promote nursing revolution.

Methods

This study will use International Classification of Functioning Disability and Health as a framework to choose suitable functions and to filter function classification standards of chronic venous leg ulcers through evidence-based systematic research. Nursing interventions related to VLU are selected based on Nursing Interventions Classification, adding other nursing activities by methods of evidence-based systematic review and clinical observation. Then, nursing interventions and function status are matched through steering committee. Finally, the Delphi survey method is adopted to make nursing program native and scientific.

Conclusions

This study is expected to be very significant and meaningful in using standardized nursing terminology. The nursing program established could better meet the needs of both patients with chronic venous leg ulcers and clinical nurses, promoting the development of wound specialist and standardized nursing language.

Open access

Ninga Sun, Ying-chaoa Zhang, Wei-guoa Zhang, En-lua Cheng and Yun-ping

Abstract

In order to effectively solve condensation and icing problems of radiosonde in low-temperature environment at high altitude, humidity sensor heated automatic alternately to remove pollution and improve the measurement accuracy. Heat experiments obtained the curve of rising temperature and responsible time on heated twin humidity sensor in normal temperature and pressure, by expanded responsible curve to obtain heated model of twin heated humidity sensor and by the analysis of heating model, use DMC and PID control for heating respectively. Simulation results show that the DMC control meets the practical requirements of measure at high altitude.

Open access

Yu Dai, Quan-yang Ma, Xiao-hua Li, Xi Zhang, Fa-ping Hu, Yun Zhang and Wei-dong Xie

Abstract

In this paper, crushability of foundry sand particles was studied. Three kinds of in-service silica sands in foundry enterprises selected as the study object, and foundry sand particles were subjected to mechanical load and thermal load during service were analyzed. A set of methods for simulating mechanical load and thermal load by milling and thermal-cold cycling were designed and researched, which were used to characterize the crushability for silica sand particles, the microstructure was observed by SEM. According to the user’s experience in actual application, the crushability of Sand C was the best and then Sand B, the last Sand A. The results indicated that mechanical load, thermal load and thermal-mechanical load can all be used to characterize the crushability of foundry sand particles. Microscopic appearances can qualitatively characterize the crushability of foundry sand particles to a certain extent, combining with the additions and cracks which are observed on the surface.

Open access

Xiao-Dong Zhang, Yi-Fang Feng, Xu Zhang, Mi Tian, Tao Wu, Peng-Fei Ye, Wei Zhang, Yue-Yun Ding, Zong-Jun Yin and Ming-Xing Chu

Abstract

To justify the function of miRNAs in reproductive regulation in swine, the expression of miR-145, miR-429 and their related genes were studied in reproductive tissues of sows. Wannan black pig and Yorkshire pigs with extremely high (n=6) and low (n=6) litter size were sampled, and real-time quantitative PCR (qPCR) was performed on tissue samples from ovaries, uterus, oviduct, hypothalamus, and pituitary. The results indicated that miR-145, miR-429, and zinc finger E-box binding homeobox 1 gene (ZEB1) were expressed significantly different in Wannan black pig and Yorkshire pigs. In pigs with different fecundity, miR-145 in the uterus was expressed significantly lower in pigs with high litter size, than in pigs with low litter size. The miR-429 expression in the oviduct and pituitary of pigs with high litter size was significantly higher compared with tissues sampled from pigs with low litter size. The ZEB1 expression in the pituitary was lower in pigs with high litter size in comparison to pigs with low litter size, while luteinizing hormone beta subunit (LHβ) showed the opposite pattern of expression. In conclusion, miR-145 and miR-429 were differently expressed in pigs with high and low litter size and might have a role in affecting litter size of sows.

Open access

Ya-Li Liu, Yao-Zhong Ding, Jun-Fei Dai, Bing Ma, Ji-Jun He, Wei-Min Ma, Jian-Liang Lv, Xiao-Yuan Ma, Yun-Wen Ou, Jun Wang, Yong-Sheng Liu, Hui-Yun Chang, Yong-Lu Wang, Qiang Zhang, Xiang-Tao Liu, Yong-Guang Zhang and Jie Zhang

Abstract

Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information.

Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay.

Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results.

Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.