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Open access

Xiao-Ming Feng, Yu Qiao, Ke Mao, Yu-Jin Hao and Chun-Xiang You

Overexpression of Arabidopsis AtmiR408 Gene in Tobacco

MicroRNA miR408 is predicted to target the transcripts for the copper proteins plantacyanin and laccase in Arabidopsis. In this study it was found that miR408 is present in tobacco also. Its accumulation was positively induced by copper deficiency, providing additional evidence for the important role of miR408 in plant responses to nutriment conditions. To examine the functions of miR408 in tobacco, transgenic tobacco lines containing the Arabidopsis AtmiR408 gene were created. Two transgenic lines with miR408 oversupply were chosen for functional characterization. The results showed that miR408 oversupply down-regulated the activities of antioxidant enzymes such as SOD, POD and CAT. As a result, transgenic lines exhibited altered chlorophyll content and seedling root growth, especially under high copper conditions. This suggests that miR408 accumulates in response to copper deficiency and influences plant growth by regulating antioxidant enzymes. It may be that miR408 exists and functions in tobacco in a conserved way similarly to miR408 in Arabidopsis.

Open access

Yu Qiao, Xiao-Ming Feng, Ze-Zhou Liu, Shuang-Shuang Wang, Yu-Jin Hao and Chun-Xiang You

Cloning and Expression Analysis of LeTIR1 in Tomato

The full-length cDNA of LeTIR1 gene was isolated from tomato with EST-based in silico cloning followed by RACE amplification. LeTIR1 contained an open reading frame (ORF) 1872 bp long, encoding 624 amino acid residues. The predicted protein LeTIR1 had one F-box motif and eleven leucine-rich repeats (LRRs), all of which are highly conserved in TIR1 proteins of other plant species. Phylogenetic analysis showed that the LeTIR1 protein shared high similarity with other known TIR1 proteins. Both sequence and phylogenetic analysis suggested that LeTIR1 is a TIR1 homologue and encodes an F-box protein in tomato. Semi-quantitative RT-PCR indicated that LeTIR1 was expressed constitutively in all organs tested, with higher expression in stem than root, leaf, flower and fruit. Its expression level was positively correlated with the auxin distribution in stem or axillary shoot, and was induced by spraying exogenous IAA.

Open access

Guang-Hua Qin, Yue-Zhong Jiang and Yu-Ling Qiao

Abstract

As one of the fast-growing tree species, hybrid poplar (Populus ssp.) has been widely planted in Shandong Province, China. While poplar tree breeding program in the past few decades focused on the development of poplar clones with fast growth rate and disease resistance, little attention was paid to the tree traits of these clones in relation to industrial uses i.e. pulpwood as well as veneer. In this paper, growth performance of hybrid poplar clones from backcrossedprogenies obtained from cross fertilization within the Poplar Aigeiros Section was evaluated and stem traits as well as wood properties in relation to industrial use of some selected clones were assessed. Of the 40 hybrid poplar clones tested in the study, A50 and B69 were prominent in growth rate at three trial sites in Shandong Province, China.Wood properties in relation to industrial uses of the two clones were also better than or comparable to the control clone. It was concluded that A50 is more suitable for pulpwood production while B69 is suitable for a wide range of high value added application such as veneer and plywood. Further research is needed to evaluate the changes of some tree traits in relation to industrial raw materials through time.

Open access

Suli Wang, Chun Qiao, Yu Zhu, Wenyi Shen, Guangsheng He and Jianyong Li

Abstract

Blast crisis (BC) is the major remaining challenge in the management of chronic myeloid leukemia (CML). The prognosis of the BC patient who carries ABL kinase mutation is very poor. One patient, with lymphoid CML-BC third time, was detected with T315A/F359I/M244V compound mutation by direct sequencing after treatment with tyrosine kinase inhibitions three years. The patient was treated with decitabine, dexamethasone, in combination with nilotinib and dasatinib. Then this patient received a complete hematologic response and cytogenetic response after two cycles of treatment.

Open access

Rui Zhang, Yu Qiao, Qiaoli Ji, Songsong Ma and Jianqiao Li

Abstract

Introduction: Reindeer are adapted to long distance migration. This species can cope with variations in substrate, especially in ice and snow environment. However, few detailed studies about reindeer hoof are available. Thus this article describes the results of studies on macro- and micro-structures of reindeer hoof.

Material and Methods: The gross anatomy of the reindeer hooves was examined. Stereo microscope (SM) and a scanning electron microscope (SEM) were used to observe four key selected positions of reindeer hooves. Moreover, element contents of the three selected positions of reindeer hooves were analysed using the SEM equipped with energy dispersive spectroscope.

Results: Hoof bone structures were similar to other artiodactyl animals. In the microscopic analysis, the surfaces of the ungula sphere and ungula sole presented irregular laminated structure. Ungula edge surfaces were smooth and ungula cusp surfaces had unique features. Aside from C, O, and N, reindeer hooves contained such elements as S, Si, Fe, Al, and Ca. The content of the elements in different parts varied. Ti was the particular element in the ungula sole, and ungula edge lacked Mg and S which other parts contained.

Conclusion: The macro- and micro-structures of the reindeer hooves showed high performance of skid and abrasion resistance. It is most probably essential to the long distance migration for the animals.

Open access

Ji Yimu, Kuang Zizhuo, Pan Qiao Yu, Sun Yanpeng, Kang Jiangbang and Huang Wei

Abstract

The GPGPU (General Purpose Graphics Processing Units) have become a whole new area for research due to the fast development of GPU hardware and programming tools, such as CUDA (Compute Unified Device Architecture). Here we have made a research on CUDA and its applications in the field of scientific computation, as organ electronics. We propose one solution of the parallel computation in global optimization of the physical characteristics in organ electronics with Monte Carlo, and one cloud service architecture for parallel computation of organ electronics was designed. Finally, one case of computing the organ molecule moving orbit was implemented based on the above solution and architecture, and has got a good display by the cloud service.

Open access

Guang-Quan Chen, Liang Yi, Xing-Yong Xu, Hong-Jun Yu, Jian-Rong Cao, Qiao Su, Lin-Hai Yang, Yong-Hang Xu, Jun-Yi Ge and Zhong-Ping Lai

Abstract

It has been suggested that the standardized growth curve (SGC) method can be used to accurately determinate equivalent dose (De) and reduce measurement time. However, different opinions regarding the applicability of the SGC method exist. In this paper, we evaluated quartz OSL SGCs of marine and coastal sediments of different grain sizes and different cores in the south Bohai Sea in China, and tested their applicability to the determination of De values. Our results suggested as follows: (1) The SGC method is applicable to both multiple- and single-aliquot regenerative-dose (MAR and SAR) protocols of OSL dating and efficiently provides reliable estimates of De. (2) Finesand quartz of different palaeodoses showed highly similar dose-response curves and an SGC was developed, but old samples using the SGC method have large uncertainties. (3) For coarse-silt quartz, two different types of dose-response curves were recorded: low-dose (≤60Gy) and high-dose (≥100Gy). The growth curves of low-dose quartz were similar to each other, facilitating the use of SGC in De estimations, but errors tended to be larger than those obtained in the SAR method. For high-dose (100–300Gy) quartz, the SGC was also found to be reliable, but there was large uncertainty in De (>300Gy) estimation. We suggest that SGC could be employed for the dating of marine and coastal sediments dating using either MAR or SAR OSL protocol and either fine-silt, coarse-silt or fine-sand quartz.