Yong-jie Wang, Yi-bo Wang, Dun-wei Du and Yan-ping Bai
Xin-Hua Wang, Yu-Lin Jiao, Yong-Chao Niu and Jie Yang
Traditional wavelet denoising method cannot eliminate complex high-pressure pipe signals effectively. In the updated wavelet adaptive algorithm, this thesis defines the constraints in order to reconstruct the signals accurately. According to the minimum mean square error criterion, the results predict the weight coefficient and get the optimal linear predictive value. Adopting the improved algorithm under the same condition, this thesis concluded that Db6 increased the complexity of wavelet algorithm by 50% by comparative experiments. It will be more conducive to the realization of hardware and the feasibility of real-time denoising. Dual adaptive wavelet denoising method improved SNR by 50%. This denoising method will play a key role in the detection rate of high-pressure pipe in the online leakage detection system.
Ya-Li Liu, Yao-Zhong Ding, Jun-Fei Dai, Bing Ma, Ji-Jun He, Wei-Min Ma, Jian-Liang Lv, Xiao-Yuan Ma, Yun-Wen Ou, Jun Wang, Yong-Sheng Liu, Hui-Yun Chang, Yong-Lu Wang, Qiang Zhang, Xiang-Tao Liu, Yong-Guang Zhang and Jie Zhang
The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information.
Material and Methods
A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay.
The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results.
A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.
Chun Xie, Yi-Xuan Hou, Yu-Ting Zhao, Xue-Hui Cai, Cai-Ying Li, Pei-Feng Li, Yun-Zhang Li, Xue Su, Xiu-Wei Yue, Shu-Jie Wang, Yong-Gang Liu, Wei-Jun Yang, Cong-Li Yuan, Li Cu, Xiu-Guo Hua and Zhi-Biao Yang
Five pathogen-free miniature pigs (minipigs) were infected with the virulent strain SH08 of Streptococcus suis 2 (SS2) by intramuscular injection. The pigs died consecutively within 72 h after the challenge. An additional five non-infected pigs were euthanised and used as controls. Microstructural observations showed that degeneration, bleeding, congestion, cellular necrosis, and an increase in inflammatory cells were present in all organs and tissues except the brain. Ultrastructural observations revealed mitochondrial vacuolation and malformed or missing cristae, indicating that infection of minipigs with strain SH08 of SS2 can lead to extensive lesions in major internal organs and tissues. The findings also demonstrated that the minipig is a useful model for the study of SS2 infection.