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Yanqing Wang, Xiaolei Wang, Yu Jiang, Yaowen Liang and Ying Liu

Abstract

A good assignment of code reviewers can effectively utilize the intellectual resources, assure code quality and improve programmers’ skills in software development. However, little research on reviewer assignment of code review has been found. In this study, a code reviewer assignment model is created based on participants’ preference to reviewing assignment. With a constraint of the smallest size of a review group, the model is optimized to maximize review outcomes and avoid the negative impact of “mutual admiration society”. This study shows that the reviewer assignment strategies incorporating either the reviewers’ preferences or the authors’ preferences get much improvement than a random assignment. The strategy incorporating authors’ preference makes higher improvement than that incorporating reviewers’ preference. However, when the reviewers’ and authors’ preference matrixes are merged, the improvement becomes moderate. The study indicates that the majority of the participants have a strong wish to work with reviewers and authors having highest competence. If we want to satisfy the preference of both reviewers and authors at the same time, the overall improvement of learning outcomes may be not the best.

Open access

Hao Pan, Han-Bing Wang, Yi-Bin Yu, Bing-Chao Cheng, Xiao-Yu Wang and Ying Li

Abstract

Advantages of the supercritical fluid (SCF) process compared to the conventional solution stirring method (CSSM) in the preparation of daidzein-hydroxypropyl-β-cyclodextrin (HPβCD) complexes were investigated. Formation of daidzein/ HPβCD inclusion complexes was confirmed by Fourier transformed-infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Particle size, inclusion yield, drug solubility and dissolution of daidzein/HPβCD complexes were evaluated. Compared to CSSM, the SCF process resulted in higher inclusion yield and higher solubility. Also, extended dissolution of daidzein from the SCF processed HPβCD inclusion complexes was observed, with only 22.94 % released in 45 min, compared to its rapid release from those prepared by CSSM, with 98.25 % drug release in 15 min. This extended release of daidzein from SCF prepared inclusion complexes was necessary to avoid drug precipitation and improve drug solubilisation in the gastrointestinal tract. The results showed that the SCF process is a superior preparation method for daidzein-hydroxypropyl-β-cyclodextrin complexes.

Open access

Bo Hu, Yu-kun Jin, Wan-jiang Gu, Jun Liu, Hua-qin Qin, Chong Chen and Ying-yu Wang

Open access

Hong-Yu Niu, Wan-Hui Ye, Zheng-Feng Wang, Ying Chen, Hong-Lin Cao, Lin-Fang Wu and Zhang-Ming Wang

Abstract

Schima superba is a common dominant tree species in evergreen broad-leaved forest in subtropical China. Despite its multiple usages in wood industry, reforestation and traditional Chinese medicine, its genetic diversity is poorly studied. To help studying its genetic diversity and structure in the future, after microsatellite enrichment and screening, we identified 16 microsatellites in S. superba. These markers showed polymorphism in three populations. The number of alleles per locus ranged from 3 to 32 with a mean of 14. Within populations, the observed and unbiased expected heterozygosities ranged from 0.048 to 0.926 and from 0.048 to 0.949, respectively. The newly developed 16 microsatellites will be useful for investigating the genetic diversity and structure from large scale patterns to fine-scale structures in this species.

Open access

Lu-Lu Wang, Shi-Ying Lu, Pan Hu, Bao-Quan Fu, Yan-Song Li, Fei-Fei Zhai, Dan-Di Ju, Shi-Jun Zhang, Bing Su, Yu Zhou, Zeng-Shan Liu and Hong-Lin Ren

Abstract

Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of Brucella and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and Brucella infection.

Material and Methods: The target open reading frame (ORF) DNAs of Prdx6 with a single active centre were prepared using gene splicing by overlap extension PCR (SOE-PCR), and the recombinant eukaryotic expression plasmids inserted by Prdx6 with the single activity centre were constructed and transfected into murine Raw264.7 macrophages. The glutathione peroxidase activity and phospholipase A2 activity of the constructed Prdx6 were examined.

Results: The core centres (Ser32 and Cys47) of Prdx6 were successfully mutated by changing the 94th nucleotide from T to G and the 140th nucleotide from G to C in the two enzyme activity cores, respectively. The constructed recombinant plasmids of Prdx6 with the single active centre were transfected into murine macrophages showing the expected single functional enzyme activity, which MJ33 or mercaptosuccinate inhibitors were able to inhibit.

Conclusion: The constructed mutants of Prdx6 with the single activity cores will be a benefit to further study of the biological function of Prdx6 with different enzyme activity.

Open access

Chun Xie, Yi-Xuan Hou, Yu-Ting Zhao, Xue-Hui Cai, Cai-Ying Li, Pei-Feng Li, Yun-Zhang Li, Xue Su, Xiu-Wei Yue, Shu-Jie Wang, Yong-Gang Liu, Wei-Jun Yang, Cong-Li Yuan, Li Cu, Xiu-Guo Hua and Zhi-Biao Yang

Abstract

Five pathogen-free miniature pigs (minipigs) were infected with the virulent strain SH08 of Streptococcus suis 2 (SS2) by intramuscular injection. The pigs died consecutively within 72 h after the challenge. An additional five non-infected pigs were euthanised and used as controls. Microstructural observations showed that degeneration, bleeding, congestion, cellular necrosis, and an increase in inflammatory cells were present in all organs and tissues except the brain. Ultrastructural observations revealed mitochondrial vacuolation and malformed or missing cristae, indicating that infection of minipigs with strain SH08 of SS2 can lead to extensive lesions in major internal organs and tissues. The findings also demonstrated that the minipig is a useful model for the study of SS2 infection.