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Open access

Qiong Yi, Xin Li, Yuan-Fang Li, Hang Yang, Xiao-Yi Zhang, Zhe Ma and Lu Wang

Abstract

Introduction: The effects of Jin-Ying-Tang (JYT) on Toll-like Receptor 4 (TLR4) signalling transduction of lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (MECs) in vitro were examined. Material and Methods: The cytotoxicity of JYT (0.06-62.50 mg/mL) on mouse MECs was determined by MTT assay. The MECs were co-cultured with LPS in the presence or absence of JYT (39.10 μg/mL, 391 μg/mL, 3910 μg/mL). The concentrations of interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) in the culture supernatants were detected by ELISA. The mRNA expression of TLR4 and downstream TLR4 signalling molecules such as myeloid differentiation factor 88 (MyD88), tumour necrosis factor receptor associated factor 6 (TRAF-6), inhibitor κB (IκB), and nuclear factor κB inducing kinase (NIK) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Results: The results showed that the IC50 of JYT on MECs was 12.25 mg/mL and JYT could significantly decrease the concentrations of IL-6 and TNF-α in LPS-stimulated MECs (P < 0.05). The mRNA expression of TLR4, MyD88, TRAF-6, IκB, and NIK was also significantly decreased when the LPS-stimulated MECs were cocultured at appropriate concentrations of JYT (P < 0.05, P < 0.01). Conclusion: These observations indicate a potential mechanism through which JYT attenuates the systemic inflammatory response to LPS-stimulated mouse mammary epithelial cells by inhibiting the activation of TLR4/MyD88/ TRAF-6/NIK pathway at the mRNA level.

Open access

Yi Xu, Wei-wei Fu, Shi Chen, Yue-fang Dong, Ke-ke Gu and Li-ping Wang

Open access

Tong-Sheng Wang, Xiu-Li Su, Yi-Min Mao and Yu-Xia Sun

Abstract

Background: Despite important advances in the diagnosis and treatment of acute pulmonary embolism (APE), diagnosis of pulmonary embolism (PE) is difficult in patients with chronic obstructive pulmonary disease (COPD) and exacerbation.

Objective: We evaluated PE in patients with chronic obstructive pulmonary disease and exacerbations of unknown origin.

Methods: Two-hundred and eight patients with COPD and severe exacerbations were studied. All patients had CT pulmonary angiography (CTPA) and lower limb ultrasonography. Arterial blood gas measurements, D-dimers and endothelin-1 (ET-1) levels were recorded.

Results: The frequency of PE was 33%. The following were more common in the PE group (χ2 = 4.32-6.79, mean p < 0.05): immobilization ≥ 7 days; a ≥ 1 cm difference in edema of the lower limbs; deep venous thrombosis; syncope; S1Q3T3 syndrome; and a decrease in PaCO2 ≥ 5 mm Hg. Plasma D-dimers and ET-1 levels were significantly higher in the PE group. Risk factors identified from logistic regression analysis were immobilization ≥ 7 days, ≥ 1 cm difference in lower limb edema, and deep venous thrombosis.

Conclusions: Overall, 33% of 208 patients had a PE, and the risk was greater in those who had been immobilized, those who had a ≥ 1 cm difference in edema of the lower limbs, and those who had a deep venous thrombosis.

Open access

Chang-Liang He, Qiong Yi, Yuan-Fang Li, Hang Yang and Lu Wang

Abstract

Mammary epithelial cells (MECs) from Kunming mice were isolated and stimulated in vitro with 10 μg/mL of Escherichia coli lipopolysaccharide (LPS). The release of tumour necrosis factor α (TNF-α) and interleukin-8 (IL-8) into culture supernatants was measured by ELISA. Furthermore, blocking experiments with Toll-like receptor 2 (TLR2) and TLR4 antibodies were performed to verify whether cytokine secretion depended on LPS-induced activation of TLR2 or TLR4. The results revealed that LPS-stimulated mouse MECs significantly secreted TNF-α and IL-8. Blocking of the TLR4 pathway inhibited the secretion of TNF-α and IL-8, while inhibition of LPS-induced TNF-α and IL-8 production was not observed when TLR2 was blocked. Thus, TLR4 can mediate the LPS-induced expression of cytokines such as TNF-α and IL-8 in mouse MECs.

Open access

Liang-xiong Dong, Yi-ran Shi and Shao-hua Wang

Abstract

The anti-impact ability of shafting affects stability and security of the ship power transmission directly. Moreover, it also cannot be ignored that the rub-impact loads have influence on the torsion vibration of ship shafting. In order to solve the problem of engineering application of reliability assessment under rub-impact loads, a test rig with rubbing generator is established. By carrying out the integrative analysis, the torsional vibration characteristics, such as vibration amplitude and orbit of axle center under the rub impact load are studied. According to the rub-impact conditions obtained through numerical simulation, the experimental verification is carried out on the test rig with rubbing generator. The results show that it is not obvious the influence of rub-impact loads upon the shafting torsion vibration except in special working conditions, that can be simulated by the rubbing generator. The maximum amplitude of torsional vibration is influenced by the radial rigidity as well as the friction coefficient of rubbing body, and the degree of influence is difference under conditions of continuous rubbing and serious rubbing. By adjusting the rigidity of stern bearing, the influence of rub-impact upon shafting can be weaken, which provides a theoretical reference for the safety evaluation of ship shafting.

Open access

Fugen Gu, Jia Ning, Huimin Fan, Chunzhi Wu and Yi Wang

Abstract

Simvastatin is poorly bioavailable because it is practically insoluble in water and shows dissolution rate-limited absorption. Solubilizing effects of several β-cyclodextrin (βCD) derivatives such as HPβCD, SBEβCD and DMβCD on simvastatin in aqueous solution were investigated using the phase solubility technique. The solubility diagram of simvastatin with each βCD derivative could be classified as AL-type, indicating soluble complex formation of 1:1 stoichiometry. Among the above βCD derivatives DMβCD was found to be the ideal complexing agent for improving drug solubility. The simvastatin complex with DMβCD was prepared using the co-evaporation method and was then characterized by differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR) and in vitro dissolution. Dissolution and pharmacokinetic studies indicated that the simvastatin/DMβCD complex exhibited an increased dissolution rate, rapid absorption, and improved bioavailability in rats compared to free drug. Maximum plasma concentration (c max) and the time to reach it (t max) were 21.86 μg mL−1 and 1.4 h for the drug complex, 8.25 μg mL−1 and 3.0 h for free drug, respectively. Main pharmacokinetic parameters such as t max, c max were significantly different (p < 0.01) between the simvastatin complex and free drug. Bioavailability of the simvastatin complex relative to free drug was up to 167.0 %.

Open access

Xiping Liu, Dong Chen, Liang Yi, Chao Zhang and Min Wang

Abstract

Magnetic-geared permanent magnet (MGPM) electrical machine is a new type of machine by incorporating magnetic gear into PM electrical machine, and it may be in operation with low-speed, high-torque and direct-driven. In this paper, three types of MGPM machines are present, and a quantitative comparison among them is performed by finite element analysis (FEA). The magnetic field distribution, stable torque and back EMF are obtained at no-load. The results show that three types of MGPM machine are suitable for different application fields respectively according to their own advantages, such as high torque and back EMF, which form an important foundation for MGPM electrical machine research.

Open access

Yi Su, Fa-yin Wang and Xiao-li An

Abstract

This article was aimed to analyse the working condition of the coupling mechanism in Chinese shipbuilding industry cluster. Literature review was conducted to unveil the coupling mechanism of shipbuilding industry cluster, providing analysis of the present development of shipbuilding industry. A theoretical model was proposed by using the efficacy function and the capacity of the coupling coefficient while measuring the coupling of the shipbuilding industry cluster. Four coupling characteristics were found including relevant, systemic, diversity, and collaborative in the shipbuilding industry cluster. The shipbuilding industry cluster has two levels of coupling mechanism including the core layer coupling mechanism and the support layer coupling mechanism respectively. The improvement through coupling the core layer, support layer, and the coupling between two levels, is an effective way to achieve long-term stable development in Chinese shipbuilding industry cluster.