Search Results

You are looking at 1 - 2 of 2 items for

  • Author: Yevgeniya Alexandrovna Kolesnikova x
Clear All Modify Search
Open access

Lyudmila Andreevna Demidchik, Valentina Vitalyevna Lee, Larissa Yevgenyevna Muravlyova, Vilen Borisovich Molotov-Luchanskiy, Ryszhan Yemelyevna Bakirova, Dmitriy Anatolyevich Klyuyev and Yevgeniya Alexandrovna Kolesnikova

Abstract

At the present time, available views show our limited knowledge of the peculiarities of the functional status of neutrophils and their metabolism in patients with community-acquired pneumonia (CAP). The studying of changes of metabolic status of neutrophils can broaden our views about pneumonia pathogenesis and define datum points of therapeutic effect.

Purpose of our research: to define oxidative stress activity and the level of oxidative modification of proteins of neutrophils in CAP patients.

Materials and methods: neutrophils obtained from 23 patients with community-acquired pneumonia. Control group consisting of 19 healthy volunteers. The reactive carbonyl derivatives of proteins and advanced oxidation protein products were defined so as to assess the oxidative damage of proteins. The malondialdehyde and nitrite ions were assessed as being indicators of the oxidative stress. The neutrophils of CAP patients with moderate severity were characterized by a tendency of evidencing decreasing content of advanced oxidation protein products, along with the statistically important enhanced levels of carbonyl derivatives and nitrite ions, while their malondialdehyde status practically leveled off with the control and had only an insignificant trend towards growth. We have demonstrated the accumulation of carbonyl derivatives and nitrite ions in the peripheral neutrophils of CAP patients. These results give evidence of an oxidative misbalance in the cells which contributes to the aggravation of the disease.

Open access

Larissa Yevgenyevna Muravlyova, Vilen Borisovich Molotov-Luchankiy, Ryszhan Yemelyevna Bakirova, Anar Akylbekovna Turmukhambetova, Dmitriy Anatolyevich Klyuyev, Ludmila Andreevna Demidchik and Yevgeniya Alexandrovna Kolesnikova

Abstract

Recent findings have demonstrated the impaired functions of neutrophils of patients with chronic renal failure. The purpose of our research was to study oxidative modified proteins, as well as the histone spectrum in neutrophils drawn from patients with chronic kidney disease, and to estimate the ability of neutrophils to form spontaneous neutrophil extracellular traps. In this work, we have assumed that metabolic alteration in neutrophils may develop at early stages of chronic kidney disease. Materials and methods: Neutrophils obtained from patients with various stages of chronic kidney disease and degrees of chronic renal failure were used. As control, blood samples obtained from 32 healthy individuals was employed. In the examined neutrophils, advanced oxidation protein products, protein reactive carbonyl derivatives, as well as nucleosomal histones were detected. The ability of neutrophils to form spontaneous neutrophil extracellular traps was estimated. Our results have demonstrated an increase of nucleosomal histones in neutrophils of all patients with chronic kidney disease. Moreover, our work fixes the rate of growth of intracellular advanced oxidation protein products and the decreasing of protein reactive carbonyl derivatives in neutrophils from patients with chronic kidney disease. Furthermore, we demonstrate the presence of the neutrophils with altered oxidative status and the decomposition of the histone spectrum in the circulation of patients with chronic kidney disease.