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  • Author: Yasar Ergun x
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Open access

Zafer Cantekin, Yasar Ergun, Hasan Solmaz, Gamze Özge Özmen, Melek Demir and Radhwane Saidi

Abstract

Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.

Open access

Pınar Peker Akalın, Veysel Soydal Ataseven, Doğan Fırat, Yaşar Ergün, Nuri Başpınar and Oğuzhan Özcan

Abstract

The aim of this study was to determine the ceruloplasmin (Cp) and vitamin C concentrations, the total antioxidant status (TAS), and selected biochemical parameters in dairy cows spontaneously infected with bovine leukaemia virus (BLV). Of the 27 cows included in the study, 18 animals were seropositive for enzootic bovine leukosis (EBL), whereas nine cows were seronegative and were used as controls. The serum aspartate aminotransferase (AST) (P = 0.003) and Cp concentrations (P = 0.03) decreased (65.17 ± 5.03 and 7.70 ± 0.72 respectively) in BLV-infected cows, as compared to healthy animals (100.67 ± 11.50 and 10.40 ± 0.70 respectively). A slight insignificant increase in alkaline phosphatase activity and unchanged levels of alanine aminotransferase, lactate dehydrogenase, calcium, magnesium, and TAS were demonstrated in EBL cows. As the TAS and vitamin C levels remained unchanged in EBL cows, it may be suggested that ruminants may compensate for the impaired oxidative/antioxidative balance. The results obtained also indicate that BLV may suppress AST and Cp synthesis or secretion in the liver through an unknown mechanism. The mechanism of action of BLV in hepatocytes, especially on AST and Cp, requires further investigation to elucidate the immune suppression caused by oncogenic retroviruses.