X Chen, J Guo, J Huang, J Yang, T Jiang, D Sun and X Yu
Experiments were conducted from 1996 to 1998 at the Hefei Institute of Economics and Technology and at the Oriental Experimental Station of the Zhejiang Province (China). Seven F1 hybrids and three parental varieties of Oriental tobaccos were evaluated for the characteristics of photosynthetic and transpiration rates, esterase isozymes, resistance to black shank, quality and product potential from the 1996-1998 growing seasons. Tobacco leaves had higher photosynthetic rates and many differences among genotypes in the early stage of plant vigorous growth compared with more mature leaves. However, transpiration rates were lower in the younger leaves and greater in the more mature leaves. All the entries had four common bands (B1, B3, B4 and B6) of the esterase isoenzymes. Differences between entries resulted from in having or not having the B2 and B5 bands and color intensity differences of all the bands. These differences could be used to identify individual entries. The F1hybrids Samsun X Toy and Samsun X Argjiro, compared with the CK Samsun control, had obvious heterotic vigor in the characteristics of product, for yield, quality and resistance to black shank. The F1 hybrid Samsun X Toy maintained higher photosynthetic and transpiration rates in the two growth stages compared to other entries. However, the F1hybrid Samsun X Argjiro had higher photosynthetic rates and lower transpiration rates in the early growth stage and the two rates were lower in the later stage, but it maintained higher photosynthetic rates for the whole growth stage. Net photosynthetic rates had a significant positive correlation with yield product, quality and resistance to black shank of the Oriental tobacco F1hybrids.
3-Oxo-α-ionol ethyl carbonate, a precursor of megastigmatrienones was prepared by reduction of α-ionone to α-ionol, followed by esterification with ethyl chloroformate and then by oxidation with t-butyl chromate. The total yield was about 23%. Infrared (IR) and mass spectra of this compound were determined. Upon smoking, cigarettes to which 0.002% by weight of the titled compound was added had an improved and more harmonious flavor. The smoke was sweeter and had a cleaner after taste. Experimental results suggest that the title compound added to the tobacco pyrolyzes to form megastigmatrienones during smoking.
Chao Tan, Dongsheng Yang, Saibo Yu, Ke Li, Haifeng Tan, Hongmei Fan, Shitai Wang, Qian Chen, Qi Liu, Yu Zhao, Xuemin Guo, Xinxin Jia and Yong Jin
After a high-pressure processing (HPP) treatment sensory evaluation of flue-cured tobacco showed modifications. There was no significant difference (P > 0.05) between the routine chemical components (total sugar, reducing sugar, nicotine, and total nitrogen) of flue-cured tobacco after high-pressure processing treatment (HPP sample) and that of an untreated control group (CG). An overall judgement, which can be made from the observations of scanning electron microscopy (SEM), X-ray computed microtomography (micro-CT) and transmission electron microscopy (TEM), is that HPP could compress the inner tunnel and tissue gap in a flue-cured tobacco leaf. However, the ultrastructure, such as the cellular cytoskeleton, would not be changed. Compared with CG, the apparent density of the HPP sample rose by 19.3%, while the true density only rose by 1.4%. This also explained that the main effect of high-pressure processing on flue-cured tobacco was microstructure compression rather than compression on the ultrastructure level. The differences between the lamina (leaf-shaped) sample, which were caused by high-pressure processing, were reflected in terahertz time-domain spectroscopy (THz-TDS), simultaneous thermal analysis (STA), and pyrolysis gas chromatography/mass spectrometry (Py-GC/MS). When the same tests were carried out using a sample that was milled to a powder, however, these differences were nearly removed. The milling process destroyed most of the microstructure of the flue-cured tobacco lamina; therefore, the results of THz-TDS, STA, and Py-GC/MS confirmed the hypothesis: That 400 MPa high-pressure processing treatment minimally changes the ultrastructure of flue-cured tobacco and only changes its relatively larger microstructure.