A series of single-hole hollow polyester fiber (SHHPF) reinforced hydrogenated carboxyl nitrile rubber (HXNBR) composites were fabricated. In this study, the sound absorption property of the HXNBR/SHHPF composite was tested in an impedance tube, the composite morphology was characterized by scanning electron microscope (SEM), and the tensile mechanical property was measured by strength tester. The results demonstrated that a remarkable change in sound absorption can be observed by increasing the SHHPF content from 0% to 40%. In the composite with 40% SHHPF in 1 mm thickness, the sound absorption coefficient reached 0.671 at 2,500 Hz; the effective bandwidth was 1,800-2,500 Hz for sound absorption coefficient larger than 0.2. But the sound absorption property of the composite deteriorated when the SHHPF content increased to 50% in 1 mm thickness. While with 20% SHHPF proportion, the sound absorption property was improved by increasing the thickness of composites from 1 to 5 mm. Compared with the pure HXNBR of the same thickness, the tensile mechanical property of the composite improved significantly by increasing the SHHPF proportion. As a lightweight composite with excellent sound absorption property, the HXNBR/SHHPF composite has potential practical application value in the fields of engineering.
Xue Meng, Yue Sun, Hong-yan Gu, Hong-shan Wei and Xing-wang Li
Objective To clone, express and purify C12orf49 recombinant protein. To prepare rabbit anti-C12orf49 protein polyclonal antibody in order to further elucidate its biological function.
Methods PCR was used to amplify the gene C12orf49 in vitro. pET-32a (+)-C12orf49, the recombinant protein prokaryotic expression vector, was transformed into E. coli. IPTG was used as the inductive agent to obtain C12orf49 recombinant protein, and the recombinant protein was analyzed with sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. Specific polyclonal antibody was derived from rabbits that immunized by recombinant protein. ELISA and Western blot were used to test its titer and specificity, respectively. MTT cell proliferation experiment was carried out to observe effect of the protein on proliferation of HepG2 cells.
Results The C12orf49 recombinant protein was expressed in a large quantity. Data of ELISA indicated that the titer of polyclonal antibody was higher than 1:1 280 000. And the antibody also had a good specificity, confirmed by Western blot. C12orf49 recombinant protein may had a advanced effect on the proliferation of HepG2 cells.
Conclusions Using C12orf49 recombinant protein, we can obtain the polyclonal antibody with great titer and good specificity. Human novel gene C12orf49 encoded protein could promote the proliferation of HepG2 cells.
To explore the effects of “hospital–community integrated transitional care” model on quality of life in patients with chronic obstructive pulmonary disease (COPD).
A total of 117 inpatients with COPD from the Department of Respiratory Medicine in a tertiary general hospital in Nanjing were enrolled by convenience sampling from January to December in 2016 and then were divided into intervention group (n = 60) and control group (n = 57) by random number table. Patients in the intervention group accepted both routine care and hospital–community integrated transitional care for 3 months after discharge. Assessment of quality of life by telephone follow-up or interview within a week before discharge and 1, 3, and 6 months after discharge was evaluated using the Short Form-36 (SF-36) health survey questionnaire.
For a total score of quality of life, there was a significant difference between the two groups (P < 0.05): 1, 3, and 6 months after discharge. In addition, for each dimension score of quality of life, there were no significant differences (P > 0.05) except vitality dimension, 1 month after discharge, and there were significant differences in all dimensions, 3 and 6 months after discharge (P < 0.05)
Hospital–community integrated transitional care model can improve the quality of life of patients with COPD.
Yan Hong, Pascal Bruniaux, Xianyi Zeng, Kaixuan Liu, Antonela Curteza and Yan Chen
This research presented a novel method using 3D simulation methods to design customized garments for physically disabled people with scoliosis (PDPS). The proposed method is based on the virtual human model created from 3D scanning, permitting to simulate the consumer’s morphological shape with atypical physical deformations. Next, customized 2D and 3D virtual garment prototyping tools will be used to create products through interactions. The proposed 3D garment design method is based on the concept of knowledge-based design, using the design knowledge and process already applied to normal body shapes successfully. The characters of the PDPS and the relationship between human body and garment are considered in the prototyping process. As a visualized collaborative design process, the communication between designer and consumer is ensured, permitting to adapt the finished product to disabled people afflicted with severe scoliosis.
Yan-hui Chen, Heng-hui Zhang, Yan Wang, Xing-wang Xie, Ran Fei, Xue-yan Wang, Lai Wei and Hong-song Chen
Objective The forkhead transcription factor FOXP3 is a key molecular which can mediate regulatory T cells immune-related inhibitory functions. Increased levels of FOXP3-positive Tregs in peripheral blood have been proved to be associated with a less favorable prognosis in various inflammatory diseases. It is of great interest to investigate the correlation between single nucleotide polymorphism (SNP) of FOXP3 gene and the susceptibility of chronic hepatitis B (CHB).
Methods Two SNPs rs2280883 and rs3761549 of FOXP3 gene in 285 patients with CHB and 295 matched controls were analyzed by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF).
Results At rs2280883, there were no significant differences in the distribution of C and T alleles between CHB donors and healthy donors, but at rs3761549, C allele was associated with CHB (P = 0.001). Compared with healthy controls, patients with CHB had high frequencies of TT genotype (73.7%) at rs2280883 or CC genotype (73.6%) at rs3761549 of FOXP3 gene. Patients who carried rs2280883 CC genotype [OR 1.744 (95% CI 1.068 - 2.848), P = 0.011] or rs3761549 CC genotype [OR 1.633 (95% CI 1.146 - 2.327), P = 0.0001] had higher risk of suffering from CHB. Stratified analysis showed that rs3761549 CC genotype was significantly associated with high incidence of HBeAg (P = 0.019).
Conclusions These results suggested that FOXP3 gene polymorphism at rs2280883 and rs3761549 might be associated with the increased susceptibility to CHB.
The aim of this paper is to build and validate a class of energy-preserving schemes for simulating a complex modified Korteweg–de Vries equation. The method is based on a combination of a discrete variational derivative method in time and finite volume element approximation in space. The resulting scheme is accurate, robust and energy-preserving. In addition, for comparison, we also develop a momentum-preserving finite volume element scheme and an implicit midpoint finite volume element scheme. Finally, a complete numerical study is developed to investigate the accuracy, conservation properties and long time behaviors of the energy-preserving scheme, in comparison with the momentum-preserving scheme and the implicit midpoint scheme, for the complex modified Korteweg–de Vries equation.
The aim of this study was to assess the management of early mobilization (EM) in Chinese intensive care units (ICUs).
This survey used a cross-sectional, observational design. A total of 65 tertiary and secondary hospitals were enrolled by convenience sampling and investigated using self-designed questionnaires.
We identified 69 ICUs in Jiangsu, China (response rate: 94.2%). 74.2% (1,004/1,353) of the nurses and nursing managers from 65 ICUs reported mobility practice. For the mobility level, 98.1% (1,327) reported use of in-bed exercise, 5.7% (77) sitting on a side of bed, 21.7% (294) transfer to chair, and 2.4% (33) walking. The most frequently reported barriers to early mobility were unplanned extubation, nursing resource, and absence of physical therapist. Nurses’ educational backgrounds, nursing experience, the lack of nursing resources, absence of physician, and the weakness of patient were the factors that influenced ICU early rehabilitation (P<0.01).
Although implementation rates for EM in critically ill patients are high, the activity level is generally poor in most of the involved ICUs.
Mulat Alubel Abtew, Subhalakshmi Kropi, Yan Hong and Linzi Pu
The main aim of this research was to implement appropriate Statistical Process Control (SPC) techniques for quality characteristics on sewing floor of garment Industry. Among different SPC quality improvements tools, control charts have been selected. After analyzing and selecting different critical parameters based on company and customer requirements, the X-bar and R charts for variable and c-charts for attribute quality characteristics have been identified and implemented in the trouser sewing lines for quality improvement. The check points for selected control chart implementation have also been designed. Remedial action plans for the occurred special cause variations and process stability were developed. The project incorporated theoretical and on-job training schemes for different quality team members, to understand the SPC concept and its implementation procedure. After implementation, significant improvements in the sewing section were achieved. The four months analysis before and after implementation of the SPC tools showed that the rejection percentage was reduced from 9.141% to 6.4%. Successful implementation of the result of this project can significantly improve process performance of other similar manufacturing units with appropriate modification.