Lack of formal tests for assessing Persian language proficiency is one of the main problems for speech and language pathologists in Iran. The purpose of this study was to generate an item pool for a syntax comprehension test based on the characteristics of the Persian language and Iranian culture, as well as the spoken variant for 4-6 years old native Persian speaking children. We first extracted 41 syntactic structures of Persian for the syntax comprehension test, of which 8 structures were excluded in the first phase. Then, 198 items were developed for the remaining 33 syntactic structures. To determine content validity, 14 experts assessed the structures and 12 experts expressed their views on the items. Content Validity Ratio (CVR) was calculated. 24 structures with CVR > 0.50 were selected. 107 items were selected based on their CVR value (CVR > 0.47). Eleven new items were regenerated to replace items with a CVR below 0.47. According to the results, the test turned out to have a good content validity.