Search Results

You are looking at 1 - 10 of 15 items for

  • Author: Y. Song x
Clear All Modify Search
Open access

Y Sha, Q Liu, Y Wang, C Dong and L Song

Exploring Candidate Genes for Epilepsy by Computational Disease-Gene Identification Strategy

Epilepsy is a complex disease with a strong genetic component. So far, studies have focused on experimental validation or genome-wide linkage scans for epilepsy susceptibility genes in multiple populations. We have used four bioinformatic tools (SNPs3D, PROSPECTR and SUSPECTS, GenWanderer, PosMed) to analyze 16 susceptibility loci selected from a literature search. Pathways and regulatory network analyses were performed using the Ingenuity Pathways Analysis (IPA) software. We identified a subset of 48 candidate epilepsy susceptibility genes. Five significant canonical pathways, in four typical networks, were identified: GABA receptor signaling, interleukin-6 (IL-6) signaling, G-protein coupled receptor signaling, type 2 diabetes mellitus signaling and airway inflammation in asthma. We concluded that online analytical tools provide a powerful way to reveal candidate genes which can greatly reduce experimental time. Our study contributes to further experimental tests for epilepsy susceptibility genes.

Open access

K. Li, M. Song, Y. Du and X. Fang

Effect of Minor Cu Addition on the Precipitation Sequence of an As-Cast Al-Mg-Si 6005 Alloy

The whole precipitation sequences of two as-cast Al-Mg-Si 6005 alloys (containing no Cu or 0.1 wt.% Cu) at 150°C were investigated using X-ray diffraction, transmission electron microscopy and hardness examinations. The precipitation sequence of the Cu-free alloy can be expressed as: super-saturated solid solution (SSSS) → spherical G.P. zones → pre-β'β' → U2 + Si + βx → Si +βx, while that of the Cu-containing alloy can be expressed as: SSSS → spherical G.P. zones + platelet-like G.P. zones → pre-β' → β' → Q' + β + Si → Q + β + Si. A new type of β precipitate, namely βx here, has been discovered in the Cu-free alloy. The addition of minor Cu is found to accelerate the microstructural evolution by inducing the formation of a new type of platelet-like G.P. zone along {111} Al planes, and improve the hardening response at the over-aged stage by forming Q' and Q precipitates.

Open access

B. Zheng, L. Xiao, X. Wang, D. Li, Y. Lu, Y. Zhang, Q. Yan and M. Song

Abstract

To study the function of the 49 kDa excretory-secretory (ES) protein gene (P49) of Trichinella, the genes was amplified by RT-PCR from RNA of Trichinella spiralis and Trichinella nativa and several Chinese Trichinella isolates of domestic animals, and sequenced after being cloned. The amplified products of these parasites produced bands of about 950 bp. The 97.2 % to 100 % nucleotides identity and 94.3 % to 100 % identity of deduced amino acids among P49 gene of these Trichinella strains showed the close relationship of these parasites. The P49 gene of T. nativa was cloned into the BamHI site of the prokaryotic expression vector pET-30a, and the recombinant vector was expressed. The expressed product was 40.8 kDa in size. In Western blot analysis, the expressed product was reactive to sera of mice infected with T. nativa, T. spiralis and their Chinese geographical strains.

Open access

J.-H. Ha, Y.-H. Park and I.-H. Song

Abstract

Porous ceramic membranes prepared from natural materials such as diatomite, have lately attracted great interest in industrial applications due to their cost-effectiveness. In this study, we attempted to prepare an alumina coating to be deposited over a sintered diatomite-kaolin composite support layer in order to reduce the largest pore size to below 0.4 μm; such a coating could be potentially used in water treatment applications for bacterial removal.

Open access

Y. Song and X. Chai

Abstract

In this paper, a semi-analytical solution for free vibration differential equations of curved girders is proposed based on their mathematical properties and vibration characteristics. The solutions of in-plane vibration differential equations are classified into two cases: one only considers variable separation of non-longitudinal vibration, while the other is a synthesis method addressing both longitudinal and non-longitudinal vibration using Rayleigh’s modal assumption and variable separation method. A similar approach is employed for the out of- plane vibration, but further mathematical operations are conducted to incorporate the coupling effect of bending and twisting. In this case study, the natural frequencies of a curved girder under different boundary conditions are obtained using the two proposed methods, respectively. The results are compared with those from the finite element analysis (FEA) and results show good convergence.

Open access

S. Song, J. Tian, Y. Li, F. Shang, X. Kang and J. Wang

Abstract

Polyploid breeding has the potential to increase the economic secondary metabolites of Eucommia ulmoides. However, pollination with induced ploidy-mixed pollen has failed to produce polyploids (GAO, 2006). In this investigation, the morphological characterization and in vitro germination of heat-induced ploidy-mixed pollen of E. ulmoides were analysed to determine why there is no polyploid production. Heat-treated pollen grains were easily distinguished as large and small according to their length. The large pollen grains were significantly longer than both untreated and heat-treated small samples, suggesting that they were probably 2n pollen. Rather than the three germinal pores in small pollen, the large grains typically had four pores and, in some cases, shallow furrows, which might affect their germination. Although the maximal germination rates of the treated small and large pollen were not significantly different, the large pollen germinated tardily during the early stages of incubation. The small pollen maintained its growth during the incubation, but the tube growth of large pollen almost stopped after 24 h incubation. Both vegetative and generative nuclei in the large pollen moved into tubes later than in small pollen and the frequency of mitosis in generative nuclei of large pollen was low. Therefore, the tardy germination, poor tube growth, and weak activity of both vegetative and generative nuclei probably caused the poor competition of large pollen in certation. Finally, techniques to increase the competition of highploidy pollen and the prospect of 2n female gamete induction in the polyploid breeding program of E. ulmoides are discussed.

Open access

Y. J. Wang, C. F. Gao, H. P. Song and S. C. Xing

Abstract

The half elliptical hole with an edge crack in a thermopiezoelectric material is studied by using the complex variable method. First, the mapping function which maps the outside of the elliptical hole and the crack in the right half plane into the outside of a circular hole in a full plane is given by the method of conformal mapping. Then, the complex potential functions and the field intensity factors (FIF) are presented according to the boundary conditions, respectively. Some useful results can be found by numerical analysis: 1) The influence of the heat flux on FIF depends on the model of the crack; 2) The shape and the size of the hole possess a significant effect on the field distribution at the crack tip.

Open access

Y Li, K-W Choy, H-N Xie, M Chen, W-Y He, Y-F Gong, H-Y Liu, Y-Q Song, Y-X Xian, X-F Sun and Xin-Jie Chen

Abstract

This study was conducted to describe a prenatal case of congenital hydrocephalus and hemivertebrae with a 6q terminal deletion and to investigate the possible correlation between the genotype and phenotype of the proband. We performed an array-based comparative genomic hybridization (aCGH) analysis on a fetus diagnosed with congenital hydrocephalus and hemivertebrae. The deletion, spanning 10.06 Mb from 6q25.3 to 6qter, was detected in this fetus. The results of aCGH, karyotype and fluorescent in situ hybridization (FISH) analyses in the healthy parents were normal, which confirmed that the proband’s copy- number variant (CNV) was de novo. This deleted region encompassed 97 genes, including 28 OMIM genes. We discussed four genes (TBP, PSMB1, QKI and Pacrg) that may be responsible for hydrocephalus while the T gene may have a role in hemivertebra. We speculate that five genes in the 6q terminal deletion region were potentially associated with hemivertebrae and hydrocephalus in the proband.

Open access

X.P. Wang, L. Song, J. Hu, Y.P. Xia, Y. Xia, Y.X. Gao, L.C. Zhang, L.B. Magalas and Q.F. Fang

The diffusion mechanisms of lithium ions in tetragonal phase as well as in Al and Nb stabilized cubic Li7La3Zr2O12 compounds were investigated by low-frequency internal friction technique. In the cubic Li7La3Zr2O12 phase, a remarkable relaxation-type internal friction peak PC with a peak height up to 0.12 was observed in the temperature range from 15°C to 60°C. In the tetragonal phase however, the height of the PT peak dropped to 0.01. The obvious difference of the relaxation strength between the cubic and tetragonal phases is due to the different distribution of lithium ions in lattice, ordered in the tetragonal phase and disordered in the cubic phase. Based on the crystalline structure of the cubic garnet-type Li7La3Zr2O12 compound, it is suggested that the high internal friction peak in the cubic phase may be attributed to two diffusion processes of lithium ions: 96h↔96h and 96h↔24d.

Open access

J. Gao, S. Zhang, L. Qi, Y. Zhang, C. Wang, W. Song and S. Han

Abstract

The Inter-Simple Sequence Repeat (ISSR) was used in this study for genetic fingerprinting and identification of 28 important Populus L. (poplar) cultivars (varieties/ clones), and determination of the genetic relationships among these cultivars. These 28 cultivars belonged to sections Aigeiros, Tacahamaca, Leuce, Turanga, and hybrids between sections Aigeiros and Tacahamaca. Out of 27 ISSR primers tested, eight primers generated clear multiplex profiles. The best three primers produced 154 easily detectable fragments, 129 (84%) of which were polymorphic among the cultivars. Each of these 3 primers produced fingerprint profiles unique to each of the accessions studied, and thus could be solely used for their identification. Twenty-five markers, unique to 10 of the cultivars studied, were detected. These markers may be converted into cultivar-specific probes for identification purposes. Genetic relationships among the cultivars were evaluated by generating a similarity matrix based on the simple matching coefficient and the unweighted pair group method with arithmetic average (UPGMA) dendrogram. The results showed a clear-cut separation of cultivars among different sections of poplar, and were in agreement with the genealogy of the sampled cultivars. The present study shows that ISSR markers could generate abundant polymorphism, are reproducible, and are quick for characterization of poplar cultivars. In the future, the markers used in this study, in combination with other molecular techniques, could provide a useful panel of ISSR markers for largescale DNA fingerprinting of poplar cultivars and determination of the genetic relationships among these cultivars.