Wen-zhi Xing and Zhu-ping Wang
Si-wen Guo, Yu Zuo, Tao Yan and Zuo-cai Wang
Chun-yu Wei, Shuai Liu, Wen-da Yu and Zhan Wang
Wenlong He, Chao Wen and Xiaoyu Wang
Purpose: This study aims to gain insights into occupational exposure of medical staff to human immunodeficiency virus (HIV) and to provide effective precautionary measures to protect them against risks arising from blood-borne pathogens.
Methodology: Data on 46 confirmed HIV-infected patients were analyzed statistically.
Results: Medical staff were exposed to blood-borne pathogens in 45 cases, and most were female and probationary nurses. Risks of occupational exposure of medical staff to HIV increased continuously as more HIV-infected patients were admitted by hospitals each year.
Conclusion: Medical staff should receive information about HIV blood-borne pathogen infection of patients, shorten the window period for HIV exposure, and practice specified precautionary measures and cut down risks of exposure to HIV.
Wen-Yang Li and Wei Wang
Liguo Jin, Hongjie Wang, Shuo Wang, Liping Wen, Jin Zhai and Tianxin Wei
A novel zinc porphyrin (5,10,15-tri-dodecoxyphenyl-20-(4-hydroxyphenyl-azo-benzenyl)-porphyrinatozinc (tdhab-ZnP)) with benzenyl-azo-phenolic group, able to adsorb on the nanocrystalline-TiO2 film, has been synthesized. We constructed a dye-sensitized solar cell based on the nanocrystalline-TiO2 hierarchical structure film, with a power conversion efficiency of 4.15 % and a high current density of 14 mA/cm2 under AM 1.5 irradiation. UV-Vis absorption spectra measurements indicated that the tdhab-ZnP molecules formed a charge transfer complex with TiO2 nanoparticles (NPs) through the phenolic group. Cyclic voltammetry measurement showed that the charge separation resulting from the tdhab-ZnP excited singlet state to the conduction band (CB) of TiO2 and charge shifting from the I−/I3− couple to the porphyrin radical cation were thermodynamically feasible.
Yongwei Liu, Wen Wang and Yiming Hu
This paper investigates the impact of surface soil moisture assimilation on the estimation of both parameters and states in the Soil and Water Assessment Tool (SWAT) model using the ensemble Kalman filter (EnKF) method in upper Huai River basin. The investigation is carried out through a series of synthetic experiments and real world tests using a merged soil moisture product (ESA CCI SM) developed by the European Space Agency, and considers both the joint state-parameter updating and only state updating schemes. The synthetic experiments show that with joint state-parameter update, the estimation of model parameter SOL_AWC (the available soil water capacity) and model states (the soil moisture in different depths) can be significantly improved by assimilating the surface soil moisture. Meanwhile, the runoff modeling for the whole catchment is also improved. With only state update, the improvement on runoff modeling shows less significance and robustness. Consistent with the synthetic experiments, the assimilation of the ESA CCI SM with joint state-parameter update shows considerable capability in the estimation of SOL_AWC. Both the joint state-parameter update and the only state update scheme could improve the streamflow modeling although the optimal model and observation error parameters for them are quite different. However, due to the high vegetation coverage of the study basin, and the strong spatial mismatch between the satellite and the model simulated soil moisture, it is still challenging to significantly benefit the runoff estimates by assimilating the ESA CCI SM.
Yan-Ming Wang, Wen-Zheng Wang, Zhen-Lu Shao, De-Ming Wang and Guo-Qing Shi
Coal spontaneous combustion is an extremely complicated physical and chemical changing process. In order to improve the indicator gases detection technology and coal spontaneous combustion monitoring, a novel forecast method for toxic gases emission from coal oxidation at low temperature is presented in this paper. The experiment system is setup combined with frequency-domain terahertz technology and coal temperature programming device. The concentration curves of carbon monoxide and sulphur dioxide gases from coal spontaneous combustion are estimated according to molecule terahertz spectra. The influences of coal rank and oxygen supply on coal spontaneous combustion characteristics are discussed. Both carbon monoxide and sulphur dioxide gases absorption spectra show the characteristic equi-spaced absorption peaks. Results demonstrate that under the condition of lean oxygen, there exists a critical oxygen concentration in the process of coal oxidation at low temperature. Comparing with Fourier infrared spectrum testing, the presented method is highly accurate and more sensitive, especially suitable for early-stage monitoring of the indicator gases produced by coal spontaneous combustion.
Qian Wang, Xiao-guang Ren, Li-yang Xu and Wen-jing Yang
Bin Zhang, Wen-hui Lun, Xing-wang Li, Qi Wang, Jun Cheng and Yu Mao
Objective To construct prokaryotic expression vector of CFP-10 gene, and obtain recombinant protein, and the recombinant CFP-10 protein was taken as stimulus to detect specific T cell responses, to set up a method to faciliate to detect potential TB infection in China.
Methods CFP-10 was cloned into inducible prokaryotic expression vector pET-32a (+) and transfected into E. coli BL21 (DE3). After IPTG induction, the product were verified with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot hybridization were carried out to verify the antigenicity; the recombinant CFP-10 protein was taken as stimulus to detect specific T cell responses in HIV (+) persons with or without clinical manifestation of TB diseases, and HIV (-) controls with or without TB diseases.
Results The CFP-10 recombinant protein exsited in the form of inclusion body and accounted for 94% in total bacterial protein of E. coli and the molecular weight is 31 kD; Western blot confirmed the recombinant proteins had high antigenicity; our in-house ELISpot-IFN-γ assay with recombinant antigen derived from CFP-10 proteins showed significant higher frequencies in TB patients with or without HIV infection than that in the healthy controls and only HIV (+) group.
Conclusions The recombinant CFP-10 genes can be expressed successfully in prokaryotic expression system of E. coli and recombinant proteins with high antigenicity were obtained, which will set foundation for further study on their immunogenicity and bioinformatics. Our results proved that it is indeed true that some HIV positive patient have high frequencies of TB specific T cell responses, which maybe a clue to find latent TB infection in this population.