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Open access

Xue Meng, Yue Sun, Hong-yan Gu, Hong-shan Wei and Xing-wang Li


Objective To clone, express and purify C12orf49 recombinant protein. To prepare rabbit anti-C12orf49 protein polyclonal antibody in order to further elucidate its biological function.

Methods PCR was used to amplify the gene C12orf49 in vitro. pET-32a (+)-C12orf49, the recombinant protein prokaryotic expression vector, was transformed into E. coli. IPTG was used as the inductive agent to obtain C12orf49 recombinant protein, and the recombinant protein was analyzed with sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. Specific polyclonal antibody was derived from rabbits that immunized by recombinant protein. ELISA and Western blot were used to test its titer and specificity, respectively. MTT cell proliferation experiment was carried out to observe effect of the protein on proliferation of HepG2 cells.

Results The C12orf49 recombinant protein was expressed in a large quantity. Data of ELISA indicated that the titer of polyclonal antibody was higher than 1:1 280 000. And the antibody also had a good specificity, confirmed by Western blot. C12orf49 recombinant protein may had a advanced effect on the proliferation of HepG2 cells.

Conclusions Using C12orf49 recombinant protein, we can obtain the polyclonal antibody with great titer and good specificity. Human novel gene C12orf49 encoded protein could promote the proliferation of HepG2 cells.

Open access

Wei Song, Huai-yuan Liu, Bin Xiang, Hong Hu, Cheng-jiang Wang and Ling-yun Wan

Open access

Wang Hong, Qi-Sheng Liang, Lan-Ren Cheng, Xiao-Hong Li, Fu Wei, Wen-Tao Dai and Shi-Tong Li


Background: Rocuronium is an alternative to succinylcholine for rapid tracheal intubation after major thermal injury and other forms of critical illness that cause denervation changes in skeletal muscle. Rocuronium may decrease the potencies of non-depolarizing muscle relaxants.

Objectives: Examine whether potency of rocuronium changed during the first month after denervation, and investigate the effects of skeletal muscle denervation on potency of rocuronium.

Methods: The denervation mouse model was developed to create denervated individual cells from the flexor digitorum brevis of the hindfoot. The skeletal muscle cells were examined at day 0 in the innervated control and days 1, 4, 7, 14, 21, and 28 in the denervation group. Nicotinic acetylcholine receptors in the cells were activated with 30 M acetylcholine, alone or in combination with various concentrations of rocuronium. Currents were recorded with a whole-cell patch-clamp technique.

Results: Rocuronium reversibly inhibited acetylcholine-activated currents in a dose-dependent fashion at different times after denervation. The inhibition concentration for the half-maximal responses of rocuronium increased 1.2- (p >0.05), 1.8-, 2.8-, 2.3-, 2.1-, and 1.9-fold (p <0.01) at day 1, 4, 7, 14, 21, and 28 after denervation, respectively, compared to that at day 0 after denervation.

Conclusion: Rocuronium dose required to achieve satisfactory clinical effects changed at different durations after skeletal muscle denervation.

Open access

Cui-Zhen Fan, Yu-Ping Chu, Ping Wei, Hong Dai and Wenming Chen

Comparison of survival of patients receiving laparoscopic and open radical resection for stage II colon cancer

Background. The aim of the study was to compare the survival of patients receiving laparoscopic vs. open radical resection for stage II colon cancer.

Patients and methods. Two hundred and twenty patients with stage II colon cancer were enrolled from Beijing Chaoyang Hospital of Capital Medical University from January 2000 to December 2009, including 61 patients in the laparoscopic radical resection group and 159 patients in the open radical resection group. The survival data in both groups were compared using the log rank test based on Kaplan-Meier survival curves.

Results. There was no statistically significant difference in the 3-year survival (88.5% vs. 80.5%; X2=1.98, P=0.159) and the 5-year survival (81.9% vs. 69.2%; X2=1.98, P=0.159) between both groups. However, statistically significant difference was found in median overall survival (mOS), which was 102.6 (95% CI: 76.8-122.7) months in the laparoscopic group and 90.0 (95% CI: 70.4-109.6) months in the open radical resection group (X2=4.183, P=0.041). mOS was 96 (95% CI: 68.6-111.4) months and 92.6 (95% CI: 56.8-107.2) months in those with and without postoperative chemotherapy, respectively (X2=6.389, P=0.011). For patients older than 75 years the mOS was 90.0 (95% CI: 25.3-105.0) months and 83.4 (95% CI: 13.1-96.9) months in the laparoscopic and open group, respectively. The difference between the both groups was statistically significant (X2=6.191, P=0.013).

Conclusions. The mOS of patients receiving laparoscopic radical resection was better than open radical resection for stage II colon cancer, especially for patients over 75 years old.

Open access

Wei Dai, Rong Zhou, Hong Yu and Xiao-juan Li


Objective To develop an effective and specific medicine targeting hepatitis B virus (HBV) pregenome. Based on the identified accessible target sites for hammerhead ribozyme in our previous researches, a recombinant hepatitis delta virus (HDV) ribozyme was chosen and used to demonstrate the effective cleavage in vitro and in vivo.

Methods Three hammerhead ribozymes for potential target sites (S, X and C genes) and co-expression plasmid (pTr-dB, pTdδ-dB, pTrX-dB and pTrC-dB) as well as four HDV-ribozyme chimera constructs with HBV (pTdXX, pTdXC, pTdSX and pTdSC) were severally chosen to validate the inhibition of the replication and expression of HBV. The co-expression plasmids (pTdδ and pTr-Db) in physiological saline were hydrodynamically injected to mice by tail vein.

Results Compared with the group injected with pTr-dB in Huh-7 cell, hepatitis B surface antigen (HBsAg) was reduced by 31% in the group injected with pTdδ-dB, by 54%, 26%, 72% and 97% in the group injected with recombinant-ribozymes pTdSX, pTdSC, pTdXC and pTdXX, respectively. The inhibiting effects of endogenous ribozymes RzX and RzC on the HBsAg expression were 66% and 57%, respectively. Compared with the positive control, the amount of HBsAg was decreased in mice injected with pTdXX through tail vein by 88% and 96% on the second day and the third day, respectively. HBsAg was undetectable on the 6th day and could not primitively be detected on the 9th day in the sera from all mice. HBV DNA was not detected in the sera of BALB/c mice injected with pTdXX-dB, pTrX-dB or replicating-defective plasmid pHBV, while HBV DNA replication in control group could be detected on the 6th day. While HBcAg could not be detected in liver tissues of mice injected with plasmid pTdXX-dB on the 3rd day.

Conclusions Encoding regions of HBV S, C and X gene were the effective cleavage sites for hammerhead ribozyme in vitro and in vivo, which provides basis for further construction of therapeutic recombinant HDV and the development of targeting antiviral gene therapy.

Open access

Saeedeh Javar, Ahmad Said Sajap, Rozi Mohamed and Lau Wei Hong


When conducting entomological research, it is necessary to have a sufficient quantity of the desired pest. One of the pests commonly used in research is Spodoptera litura. Mass rearing of herbivorous caterpillars like S. litura requires large amounts of a suitable food source. Hence, the use of an easy-to-grow host plant would be most practical and reasonable. Centella asiatica known as ‘pegaga’ grows easily, produces plenty of leaves, and is available throughout the year in Malaysia. The suitability of C. asiatica as a food source for S. litura was evaluated by studying the biology of this pest on excised C. asiatica leaves, under laboratory conditions. S. litura completed its whole life cycle in 29 to 35 days. The mean pupal weight and the average number of eggs laid by a single female were 0.341 g and 1,930 eggs, respectively. Daily monitoring of the larval development stages as well as analysis on the width of head capsules, revealed the existence of six instars during the larval stages of S. litura. The cumulative survival rate for immature developmental stages was 80%, while the stage-specific survivorship was over 90%. This study demonstrated the use of ‘pegaga’ as a suitable new food source for when rearing S. litura larvae in the laboratory.

Open access

Qu Yuqiu, Zhang Liuyang, An Limin and Wei Hong


The effect of different organic charge transporting materials on the photoluminescence of CdSe/ZnS core/shell quantum dots has been studied by means of steady-state and time-resolved photoluminescence spectroscopy. With an increase in concentration of the organic charge transporting material in the quantum dots solutions, the photoluminescence intensity of CdSe/ZnS quantum dots was quenched greatly and the fluorescence lifetime was shortened gradually. The quenching efficiency of CdSe/ZnS core/shell quantum dots decreased with increasing the oxidation potential of organic charge transporting materials. Based on the analysis, two pathways in the photoluminescence quenching process have been defined: static quenching and dynamic quenching. The dynamic quenching is correlated with hole transporting from quantum dots to the charge transporting materials.

Open access

Wen-Zhi Zeng, Guo-Qing Lei, Hong-Ya Zhang, Ming-Hai Hong, Chi Xu, Jing-Wei Wu and Jie-Sheng Huang


For estimation of root-zone moisture content from EO-1/Hyperion imagery, surface soil moisture was first predicted by hyperspectral reflectance data using partial least square regression (PLSR) analysis. The textures of more than 300 soil samples extracted from a 900 m × 900 m field site located within the Hetao Irrigation District in China were used to parameterize the HYDRUS-1D numerical model. The study area was spatially discretized into 18,000 compartments (30 m × 30 m × 0.02 m), and Monte Carlo simulations were applied to generate 2000 different soil-particle size distributions for each compartment. Soil hydraulic properties for each realization were determined by application of artificial neural network analysis and used to parameterize HYDRUS-1D to simulate averaged soil-moisture contents within the root zone (0-40 cm) and surface (approximately 0-4 cm). Then the link between surface moisture and root zone was established by use of linear regression analysis, resulting in R and RMSE of 0.38 and 0.03, respectively. Kriging and co-kriging with observed surface moisture, and co-kriging with surface moisture obtained from Hyperion imagery were also used to estimate root-zone moisture. Results indicated that PLSR is a powerful tool for soil moisture estimation from hyperspectral data. Furthermore, co-kriging with observed surface moisture had the highest R (0.41) and linear regression model, and HYDRUS Monte Carlo simulations had a lowest RMSE (0.03) among the four methods. In regions that have similar climatic and soil conditions to our study area, a linear regression model with HYDRUS Monte Carlo simulations is a practical method for root-zone moisture estimation before sowing and it can be easily coupled with remote sensing technology.

Open access

Yu Cao, Jiang Zhang, Wei Yang, Cheng Xia, Hong-You Zhang, Yan-Hui Wang and Chuang Xu


Introduction: The predictive value of selected parameters in the risk of ketosis and fatty liver in dairy cows was determined.

Material and Methods: In total, 21 control and 17 ketotic Holstein Friesian cows with a β-hydroxybutyrate (BHBA) concentration of 1.20 mmol/L as a cut-off point were selected. The risk prediction thresholds for ketosis were determined by receiver operating characteristic (ROC) curve analysis.

Results: In the ketosis group, paraoxonase-1 (PON-1) activity and concentration of PON-1 and glucose (GLU) were decreased, and aminotransferase (AST) activity as well as BHBA and non-esterified fatty acid (NEFA) contents were increased. The plasma activity and concentration of PON-1 were significantly positively correlated with the level of plasma GLU. The plasma activity and concentration of PON-1 were significantly negatively correlated with the levels of AST and BHBA. According to ROC curve analysis, warning indexes of ketosis were: plasma PON-1 concentration of 46.79 nmol/L, GLU concentration of 3.04 mmol/L, AST concentration of 100 U/L, and NEFA concentration of 0.82 mmol/L.

Conclusion: This study showed that the levels of PON-1, GLU, AST, and NEFA could be used as indicators to predict the risk of ketosis in dairy cows.