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  • Author: Wang Zhen-fei x
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Open access

Wang Zhen-fei, Liu Li, Liang Lin and Hao Qin

Abstract

Objective

The aim of this study was to investigate the effect of Radix Glehniae on the migration and invasion abilities of lung cancer cells.

Methods

Normal bronchial cell line 16HBE and lung cancer cell line SK-MES-1 were treated with Radix Glehniae extract. Proliferation, migration, and invasion abilities were determined by Cell Counting Kit (CCK)-8, Transwell, and Matrigel assays, respectively. The expression and secretion levels of tissue inhibitor of metalloproteinases 2 were detected by quantitative PCR and enzyme-linked immunosorbent assay, respectively.

Results

Radix Glehniae extract inhibited the migration and invasion abilities of SK-MES-1 cells and enhanced TIMP2 expression and secretion by SK-MES-1 cells, without causing toxicity to 16HBE cells.

Conclusion

Radix Glehniae is useful in lung cancer treatment.

Open access

Wang Zhen-fei, Mu Yong-ping, Liang Jun-qing, Liu Yong-yan and Li Jing-quan

Abstract

Objective

This study aimed to investigate the influence of Xanthii fructus on the expression of small noncoding RNA (sncRNA) and the malignant behaviors of lung cancer cells.

Method

A549 cells were treated with Xanthii fructus extract. SncRNA expression was detected by real-time PCR. Proliferation, anchorage-independent growth, and invasion capacities were determined using Cell Counting Kit (CCK)-8, soft agar colony formation, and Matrigel assays, respectively.

Results

Xanthii fructus extract downregulated microRNA (miR)-21 expression and upregulated PIWI-interacting RNA (piRNA)55490 expression. The proliferation, anchorage-independent growth, and invasion capacities of A549 cells were strongly inhibited by the extract.

Conclusion

Xanthii fructus can inhibit the malignant behaviors of lung cancer cells.

Open access

Zhen-Hui Xin, Ya-Li Meng, Yan-Hua Wu, Jian Wang, Zhi-Ke Feng and Yan-Fei Kang

Abstract

A simple and coumarin-based fluorescence probe has been designed and synthesized with silyl group as recognition group of fluoride ions (F−) in this study. The results showed that the fluorescence intensity of the probe displayed prominent enhancement with addition of F− at 445 nm with incubation of 1 min. There was an excellent linear relationship between fluorescence intensity and fluoride concentration from 0 to 30 μM (0~0.57 ppm), which offered the important condition for the quantitative analysis. In addition, the highly selective response to fluorion, the low detection limit with 28 nM (0.532 ppb), low toxicity and bioimaging afforded an advantage for practical application and detecting fluoride in biological systerms.