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  • Author: Wan Omar Wan Maznah x
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Zoya Khan, Wan Maznah Wan Omar, Faradina Merican Mohd Sidik Merican, Asmimie Asmawarnie Azizan, Choon Pin Foong, Peter Convey, Nazalan Najimudin, Jerzy Smykla and Siti Aisyah Alias

Abstract

A filamentous benthic cyanobacteria, strain USMAC16, was isolated from the High Arctic Svalbard archipelago, Norway, and a combination of morphological, ultrastructural and molecular characterisation (16S rRNA gene sequence) used to identify to species level. Cell dimensions, thylakoid arrangement and apical cell shape are consistent with the Pseudanabaena genus description. The molecular characterisation of P. catenata gave 100% similarity with Pseudanabaena catenata SAG 1464-1, originally reported from Germany. Strain USMAC16 was cultured under a range of temperature and photoperiod conditions, in solid and liquid media, and harvested at exponential phase to examine its phenotypic plasticity. Under different culture conditions, we observed considerable variations in cell dimensions. The longest cell (5.91±0.13 μm) was observed at 15°C under 12:12 light:dark, and the widest cell (3.24±0.06 μm) at 4°C under 12:12 light: dark in liquid media. The study provides baseline data documenting the morphological variation of P. catenata in response to changing temperature regimes.

Open access

Zakeyuddin Mohd Shafiq, Md Sah Amir Shah Ruddin, Hazrin Hashim Zarul, Puteh Khaled, Mohammad Syaiful and Wan Omar Wan Maznah

Abstract

Seasonal changes of freshwater fish assemblages and environmental factors in Bukit Merah Reservoir were carried out from January-February 2013 (dry season) to March-April 2013 (wet season) by measuring several physico-chemical parameters such as dissolved oxygen (DO), water temperature, pH, water conductivity, total dissolved solids (TDS) and water clarity. 19 fish species comprising of 10 families were collected by using experimental gill nets with different mesh sizes at four different sampling stations. Mean CPUE for total catch and biomass were significantly different between dry and wet season (p < 0.05). Based on a T-test analysis, water temperature, pH and conductivity were significantly different (p < 0.05) between seasons whereas a one-way ANOVA displayed a significant difference in TDS and water clarity between sampling stations (p < 0.05), implicating that those factors did not give major influence towards other parameters in a man-made reservoir. From all fish species studied, only mean CPUE for individuals (CPUEn) of Osteochilus vittatus and Oxygaster anomalura had a significant difference between seasons (p < 0.05); probably an indicator of their migration season