Three problems in the taxonomy of Pancratium in Egypt are the lack of publications, a lack of clarity about the relationships between recently distinguished species, and the lack of markers for examining the levels and patterns of variation in rare and endemic species; the latter hinders work in plant conservation genetics. In this study we reassessed the taxonomic status of the Pancratium species of Egypt, and examined morphological and genetic variation within and between species, using specimens from different populations collected throughout its distribution range in the country. Our assessment was based on 38 macromorphological characters mainly representing vegetative parts, flowers, fruits and seeds, in addition to RAPD data. The results revealed five morphologically distinguished Pancratium species in Egypt, of which P. trianthum Herb. is newly recorded. Species identification was confirmed by two phenetic dendrograms generated with 26 quantitative morphological characters and RAPD data, while species delimitation was verified by principal component analysis. The diagnostic floral characters are those of the perianth, corona teeth, pistil, stamens, aerial scape, spathe, and number of flowers. The retrieved RAPD polymorphic bands show better resolution of the morphologically and ecologically closely allied Pancratium species (P. arabicum and P. maritimum), and also confirm the morphological and ecological divergence of P. tortuosum from the other studied species. These results are supported by the constructed UPGMA dendrogram.
Azza El-Hadidy, Monier Abd El-Ghani, Wafaa Amer and Rania Hassan
Amal Saad-Hussein, Mohgah Sh. Abdalla, Wafaa Gh. Shousha, Gehan Moubarz and Aya H. Mohamed
Aim: The study aimed to estimate oxidative role of aflatoxin B1 (AFB1) on the liver in wheat milling workers.
Materials and Methods: Case-control study was conducted to compare between the levels of AFB1/albumin (AFB1/alb), liver enzymes (ALT, AST, GGT, and ALP), P53, MDA, GST, SOD, zinc and vitamin C in 35 wheat milling workers and 40 control subjects.
Results: Statistical analysis revealed that ALT, AST, GGT, ALP, P53, MDA, GST and SOD in workers were significantly elevated compared to their controls. In the milling workers, there were significant correlations between MDA levels and the levels of AST, GGT, and P53, while, P53 was inversely correlated with GST and SOD activities. There were significant correlations between Zn levels and GGT, GST and SOD activities, between vitamin C and GST activities, and vitamin C inversely correlated with MDA.
Conclusion: The present study concluded that the oxidative stress of AFB1 elevated the MDA and the liver enzymes in wheat milling workers. GST has a crucial role in the detoxification of aflatoxin and SOD as a scavenger antioxidant increased in the workers to overcome the oxidative toxic effects of AFB1 on the liver of the workers, and roles of Zn and vitamin C were significant in activation of these processes.